The calcium content and <sup>45</sup>calcium uptake of the smooth muscle of the guinea‐pig taenia coli

Bauer, Horst; Goodford, P. J.; Hüter, J

doi:10.1113/jphysiol.1965.sp007542

Cited by 64 publications

(39 citation statements)

References 23 publications

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“…The rate of loss of Ca from taenia coli has been shown to be largely controlled by a physical process although the possibility of a slight participation of metabolism-de pendent Ca-exclusion mechanism could not be excluded (3,8), and the present data seem to support the suggestion. On the other hand, Pfaffman and Holland (7) reported that the increase in tissue Ca in hypertonically added 40 mm K solution did not change in the presence of DNP, but was decreased by ouabain, and suggested the existence of DNP sensitive Ca-extrusion pump in taenia coli.…”

Section: Discussionsupporting

confidence: 80%

Effects of Ouabain and 2,4-Dinitrophenol on Calcium Distribution and Exchange in Guinea Pig Taenia Coli in High-Potassium Solution

Karaki

Ikeda

Urakawa

1970

Japanese Journal of Pharmacology

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Previous studies have shown that Ca influx increased but Ca efflux did not change during the contracture of taenia coli induced in hypertonically added 40 mm potassium solution (1) and the results were supported by the others (2-6). Further, the movements of Ca in the muscle in high-K solution might be differently affected by ouabain and 2, 4-dinitrophenol during the abolition of the tonic tension by them (6, 7).The experiments reported here were carried out in purpose to find the nature of Ca distribution and exchange in guinea pig taenia coli in high-K solution using ouabain and 2, 4-dinitrophenol. METHODSStrips of taenia coli isolated from white male guinea pigs were suspended in an organ bath containing Tyrode solution of the following composition (mm); NaCI 136.8, KCl 2.7, CaC1, 2.5, MgCl2 1.0, NaH2PO4 0.4, NaHCO, 11.9 and glucose 5.5, saturated with 95% 02 and 5% CO, mixture at 37'C. Isotonically added 40 mm K solution (iso-40 K-S.) was made by replacing 40 mm NaCI in Tyrode solution with equimolar KC1. Tension changes were isometrically recorded with a mechanoelectro-transducer. 45Ca uptake: After pretreatment , muscle strips were soaked in iso-40 K-S. for 30 minutes, then 2.5 x 10-6 M ouabain or 1 x 10-4 M 2, 4-dinitrophenol (DNP) was added. Thirty minutes after the addition of ouabain or DNP, corresponding to zero time of Fig. 1, 45Ca (1 x 106 counts/min-ml) was added to the medium for incubation. After 5, 15 or 30 minutes incubation period, the strips were removed from the bath and radioactivities in the muscle strips were counted (1, 4). Tissue Ca: Strips were treated in the same manner as that in the preceding para graph except that 45Ca was not used and Ca content of the tissue was determined using atomic absorption spectrophotometer (1, 4). 45Ca efflux: Muscle strips were incubated in iso -40 K-S. with 45Ca (200 pCi/ml) for 180 to 200 minutes and then washed by dipping into successive tubes containing iso

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Section: Discussionsupporting

confidence: 80%

Effects of Ouabain and 2,4-Dinitrophenol on Calcium Distribution and Exchange in Guinea Pig Taenia Coli in High-Potassium Solution

Karaki

Ikeda

Urakawa

1970

Japanese Journal of Pharmacology

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“…Further, an extracellular "calcium space" of taenia coli exchanges very rapidly (T%<30 sec) (8) and it is plausible to assume that the changes in 45Ca contained in the extracellular space does not alter the above conclusion as a 4 minute wash out period was adopted in the present experiment.…”

Section: Results Shown Inmentioning

confidence: 96%

INCREASE IN 45Ca UPTAKE DURING BARIUM-INDUCED CONTRACTURE IN GUINEA PIG TAENIA COLI

1967

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Barium (Ba) is known to be a non-specific stimulant of smooth muscle and its stimulating effect has been generally explained as the direct action on the contractile mechanism.However, we reported (1, 2) that Ba-induced tension development in isolated guinea pig taenia coli was dependent on the existence of external calcium (Ca) and Ca might play an essential role in it.In the present data, changes in 45Ca uptake and total Ca content during tension development induced by 0.5 mm Ba, which was about the same magnitude as that induced by 40 mat KCI, in guinea pig taenia coli were examined.For the determination of 45Ca uptake of the muscle, 45CaCl, was added to the medium simultaneously with 40 mar K or 0.5 mm Ba and after the incubation periods, the muscle was washed for 4 minutes with a non-radioactive medium of the same composition as the incubation medium.Samples of the muscle were ashed for the following analysis. 45Ca was counted by gas flow counter (Aloka window type Model PS-13) and total Ca content of the muscle was determined using Perkin Elmer Model 303 atomic absorption spectrophotometer.High-K medium was made by adding KCI crystals to the normal Tyrode solution (2.5 mm Ca) to make final concentration of 42.7 mm KC1.

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“…It has been shown that the tissue calcium content of taenia coli rises with cooling and anoxia (7), and the rate of calcium loss from taenia coli was largely controlled by a physical process (8). This suggests a possible Ca-extrusion mechanism which is dependent on metabolic energy.…”

mentioning

confidence: 97%

Role of glycolysis in the tension development under anoxia in guinea pig taenia coli.

Nasu¹,

Yui²,

Nakagawa³

et al. 1982

Jpn.J.Pharmacol

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Abstract-The role of glycolysis in the tension development under anoxic conditions in a high-K medium was studied in the intestinal smooth muscle of guinea pig taenia coll. After exposure to the high-K medium (isotonic , 60 mM) under normal oxygen for 30 min , the muscles were exposed to a high-K medium bubbled with N2 gas . The tonic contraction decreased gradually to about 10% of the original level. Glucose was then cumulatively added to the high-K medium under anoxia.

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The calcium content and ⁴⁵calcium uptake of the smooth muscle of the guinea‐pig taenia coli

Cited by 64 publications

References 23 publications

Effects of Ouabain and 2,4-Dinitrophenol on Calcium Distribution and Exchange in Guinea Pig Taenia Coli in High-Potassium Solution

Effects of Ouabain and 2,4-Dinitrophenol on Calcium Distribution and Exchange in Guinea Pig Taenia Coli in High-Potassium Solution

INCREASE IN 45Ca UPTAKE DURING BARIUM-INDUCED CONTRACTURE IN GUINEA PIG TAENIA COLI

Role of glycolysis in the tension development under anoxia in guinea pig taenia coli.

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The calcium content and 45calcium uptake of the smooth muscle of the guinea‐pig taenia coli

Cited by 64 publications

References 23 publications

Effects of Ouabain and 2,4-Dinitrophenol on Calcium Distribution and Exchange in Guinea Pig Taenia Coli in High-Potassium Solution

Effects of Ouabain and 2,4-Dinitrophenol on Calcium Distribution and Exchange in Guinea Pig Taenia Coli in High-Potassium Solution

INCREASE IN 45Ca UPTAKE DURING BARIUM-INDUCED CONTRACTURE IN GUINEA PIG TAENIA COLI

Role of glycolysis in the tension development under anoxia in guinea pig taenia coli.

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The calcium content and ⁴⁵calcium uptake of the smooth muscle of the guinea‐pig taenia coli