The C5 protein encoded by Ageratum leaf curl Sichuan virus is a virulence factor and contributes to the virus infection

Li, Pengbai; Meng, Qiyuan; Yu, Huabin; Wu, Gentu; Li, Mingjun; Qing, Ling

doi:10.1111/mpp.13103

Cited by 16 publications

(28 citation statements)

References 35 publications

(58 reference statements)

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“…Recombination among the sequence of all datasets might provide a high rate of evolution, rapid multiplication, and expansion of the host range. Various studies have revealed the high frequencies of recombination in begomovirus populations [ 36 ] and for ssDNA viruses which use a rolling circle replication mechanism, non-random sites of recombination events are a conserved trait [ 6 , 7 , 37 ]. In this study we observed at least one recombination breakpoint in analyzed datasets of DNA-A component and associated satellite molecules which strengthen the previous studies.…”

Section: Discussionmentioning

confidence: 99%

“…Another crucial factor that significantly contributes to diversity of plant viruses is recombination. Recombination contributes to the evolution of a number of virus species and has been extensively identified in the genomes of many geminiviruses [ 1 , 6 , 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

“…DNA-A encodes six virus proteins. Four proteins are encoded by the complementary sense strand: replication associated protein (Rep/AC1), transcription activator protein (TrAP/AC2), replication enhancer protein (REn/AC3) and AC4 while virions sense strand encodes for coat protein (CP/AV1) and pre-coat protein (pre-CP/AV2), in some begomoviruses species AC5/C5 protein were also observed [ 6 , 14 ]. DNA-B is involved in the systemic (cell to cell) and local (nucleus to cytoplasm) movements of begomovirus and involved proteins aremovement protein (MP) and nuclear shuttle protein (NSP) respectively [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

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Role of Diversity and Recombination in the Emergence of Chilli Leaf Curl Virus

et al. 2022

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Chilli leaf curl virus (ChiLCV), (Genus Begomovirus, family Geminiviridae) and associated satellites pose a serious threat to chilli production, worldwide. This study highlights the factors accountable for genetic diversity, recombination, and evolution of ChiLCV, and associated chilli leaf curl alphasatellite (ChiLCA) and chilli leaf curl betasatellite (ChiLCB). Phylogenetic analysis of complete genome (DNA-A) sequences of 132 ChiLCV isolates from five countries downloaded from NCBI database clustered into three major clades and showed high population diversity. The dN/dS ratio and Tajima D value of all viral DNA-A and associated betasatellite showed selective control on evolutionary relationships. Negative values of neutrality tests indicated purified selection and an excess of low-frequency polymorphism. Nucleotide diversity (π) for C4 and Rep genes was higher than other genes of ChiLCV with an average value of π = 18.37 × 10−2 and π = 17.52 × 10−2 respectively. A high number of mutations were detected in TrAP and Rep genes, while ChiLCB has a greater number of mutations than ChiLCA. In addition, significant recombination breakpoints were detected in all regions of ChiLCV genome, ChiLCB and, ChiLCA. Our findings indicate that ChiLCV has the potential for rapid evolution and adaptation to a range of geographic conditions and could be adopted to infect a wide range of crops, including diverse chilli cultivars.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Role of Diversity and Recombination in the Emergence of Chilli Leaf Curl Virus

et al. 2022

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“…Geminiviruses are a class of DNA viruses with circular single-stranded genomes that encode limited numbers of proteins [ 1 – 4 ]. Geminiviruses recruit host factors to ensure their genome replication, virion assembly, movement, insect transmission, and other processes [ 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…Geminiviruses recruit host factors to ensure their genome replication, virion assembly, movement, insect transmission, and other processes [ 2 ]. Plants infected with geminiviruses often develop disease symptoms such as yellow veins, leaf curling, enation, and curly shoots [ 4 – 7 ]. During virus-plant arms races, host plants have evolved specific proteins targeting various viral infection steps.…”

Section: Introductionmentioning

confidence: 99%

Geminivirus C4 proteins inhibit GA signaling via prevention of NbGAI degradation, to promote viral infection and symptom development in N. benthamiana

Guo

Lang

et al. 2022

PLoS Pathog

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The phytohormone gibberellin (GA) is a vital plant signaling molecule that regulates plant growth and defense against abiotic and biotic stresses. To date, the molecular mechanism of the plant responses to viral infection mediated by GA is still undetermined. DELLA is a repressor of GA signaling and is recognized by the F-box protein, a component of the SCFSLY1/GID2 complex. The recognized DELLA is degraded by the ubiquitin-26S proteasome, leading to the activation of GA signaling. Here, we report that ageratum leaf curl Sichuan virus (ALCScV)-infected N. benthamiana plants showed dwarfing symptoms and abnormal flower development. The infection by ALCScV significantly altered the expression of GA pathway-related genes and decreased the content of endogenous GA in N. benthamiana. Furthermore, ALCScV-encoded C4 protein interacts with the DELLA protein NbGAI and interferes with the interaction between NbGAI and NbGID2 to prevent the degradation of NbGAI, leading to inhibition of the GA signaling pathway. Silencing of NbGAI or exogenous GA3 treatment significantly reduces viral accumulation and disease symptoms in N. benthamiana plants. The same results were obtained from experiments with the C4 protein encoded by tobacco curly shoot virus (TbCSV). Therefore, we propose a novel mechanism by which geminivirus C4 proteins control viral infection and disease symptom development by interfering with the GA signaling pathway.

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The novel C5 protein from tomato yellow leaf curl virus is a virulence factor and suppressor of gene silencing

et al. 2022

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Tomato yellow leaf curl virus (TYLCV) is known to encode 6 canonical viral proteins. Our recent study revealed that TYLCV also encodes some additional small proteins with potential virulence functions. The fifth ORF of TYLCV in the complementary sense, which we name C5, is evolutionarily conserved, but little is known about its expression and function during viral infection. Here, we confirmed the expression of the TYLCV C5 by analyzing the promoter activity of its upstream sequences and by detecting the C5 protein in infected cells by using a specific custom-made antibody. Ectopic expression of C5 using a potato virus X (PVX) vector resulted in severe mosaic symptoms and higher virus accumulation levels followed by a burst of reactive oxygen species (ROS) in Nicotiana benthamiana plants. C5 was able to effectively suppress local and systemic post-transcriptional gene silencing (PTGS) induced by single-stranded GFP but not double-stranded GFP, and reversed the transcriptional gene silencing (TGS) of GFP. Furthermore, the mutation of C5 in TYLCV inhibited viral replication and the development of disease symptoms in infected plants. Transgenic overexpression of C5 could complement the virulence of a TYLCV infectious clone encoding a dysfunctional C5. Collectively, this study reveals that TYLCV C5 is a pathogenicity determinant and RNA silencing suppressor, hence expanding our knowledge of the functional repertoire of the TYLCV proteome.

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The C5 protein encoded by Ageratum leaf curl Sichuan virus is a virulence factor and contributes to the virus infection

Abstract: Geminiviruses are single-stranded circular DNA viruses that infect many plant species and cause severe economic losses worldwide (Fauquet et al., 2003;Harrison, 1985;Navas-Castillo et al., 2011). Based on the current taxonomic criteria, geminiviruses are

Cited by 16 publications

References 35 publications

Role of Diversity and Recombination in the Emergence of Chilli Leaf Curl Virus

Role of Diversity and Recombination in the Emergence of Chilli Leaf Curl Virus

Geminivirus C4 proteins inhibit GA signaling via prevention of NbGAI degradation, to promote viral infection and symptom development in N. benthamiana

The novel C5 protein from tomato yellow leaf curl virus is a virulence factor and suppressor of gene silencing

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