The Broad Aglycon Specificity of α-L-Fucosidases from Marine Gastropods

Demonstration and partial characterization of endo‐N‐acetyl‐β‐d‐glucosaminidase in human tissues

“…The human endo#acetyl-/3-D-glucosaminidase activity found had a similar pH optimum to those described in rat tissues [2,4,5]. Furthermore, the ratios of the two activities (towards GP-IIIA and ASTF) differed in the various tissues of the same individual, indicating the presence of two separate enzyme proteins, as was suggested for the rat [ 5 1.…”

“…Endoglucosaminidase activity towards oligomannosidic glycans was reported in rat and pig tissues [2]. This finding was confirmed [4], localizing this type of enzymatic activity in the cytosolic fraction of rat liver and kidney, with an oligomannosidic substrate derived from ovalbumin.…”

“…It has been postulated that the biological degradation of the oligosaccharide part of the serum type glycoproteins would be initiated by the splitting of the di-N-acetyl chitobiose moiety by an endo-Nacetyl-/3-D-glucosaminidase (EC 3.2.1.96 endoglucosaminidase) [ 1,2]. This hypothesis is sustained by the finding of oligosaccharide chains of various lengths and compositions in urine and tissues of patients, suffering from lysosomal storage disorders as in sialidosis 131.…”

“…In the same tissue activity was also shown towards a glycopeptide isolated from asialotransferrin, a substrate of the N-acetyl-lactosaminic type [5]. To obtain support for the hypothesis [ 1,2], it was of interest to see whether endoglucosaminidase activity could be detected in human tis- type is demonstrated in human tissues. Furthermore, some properties of the enzyme (pH optimum, influence of thiol reagents and stability) in human kidney are described.…”

Purification and Characterization of a Novel α-l-Fucosidase fromFusarium oxysporumGrown on Sludge

“…also showed this type of specificity. On the other hand, the rt-L-fucosidases from mammalian tissues,8 '" 10) abalone 11 ) and a marine gastropod 12 ) readily hydrolyzed p-nitrophenyl rt-L-fucoside and also liberated fucose slowly from mucin, but seemed not to hydrolyze blood group substances.…”