The Breeding of Phalaenopsis Hybrids

Yuan, S.X.; Bolaños-Villegas, Pablo; ChinYi, Tsao; Chen, Fure-Chyi

doi:10.1007/978-3-030-66826-6_2

Cited by 4 publications

(4 citation statements)

References 60 publications

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“…Remarkably, many of them (e.g., MER3 , MSH4 and MSH5 ) show rapid loss of duplicates following evolutionary events of WGD in angiosperms [ 47 ]. One might envision the use of CRISPR-Cas9 editing to trim the number of MSH4 duplicates in valuable orchid polyploids to stabilize meiosis [ 16 ]. In fact, in allopolyploids, the ZMM pathway could be targeted to reduce the number of crossovers to the bare minimum, one per chromosome pair to prevent inter-homoeologue crossover formation [ 47 ].…”

Section: Polyploidization and Recombinationmentioning

confidence: 99%

“…Traditionally, fertility can be restored in these orchid hybrids by doubling the number of chromosomes with antimitotic agents such as colchicine and oryzalin during tissue culture [ 13 ]; the result is an allotetraploid [ 15 ] (see Table 1 ). Ideally, the process induces disomic pairing of homologous chromosomes during meiosis I and the creation of balanced gametes [ 13 ], but unfortunately, chromosomes may undergo structural rearrangements under the influence of colchicine, which may reduce fertility [ 16 ].…”

Section: Introductionmentioning

confidence: 99%

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Advances and Perspectives for Polyploidy Breeding in Orchids

Bolaños-Villegas

Chen

2022

Plants

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The orchid market is a dynamic horticultural business in which novelty and beauty command high prices. The two main interests are the development of flowers, from the miniature to the large and showy, and their fragrance. Overall organ size might be modified by doubling the chromosome number, which can be accomplished by careful study of meiotic chromosome disjunction in hybrids or species. Meiosis is the process in which diploid (2n) pollen mother cells recombine their DNA sequences and then undergo two rounds of division to give rise to four haploid (n) cells. Thus, by interfering in chromosome segregation, one can induce the development of diploid recombinant cells, called unreduced gametes. These unreduced gametes may be used for breeding polyploid progenies with enhanced fertility and large flower size. This review provides an overview of developments in orchid polyploidy breeding placed in the large context of meiotic chromosome segregation in the model plants Arabidopsis thaliana and Brassica napus to facilitate molecular translational research and horticultural innovation.

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Section: Polyploidization and Recombinationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Advances and Perspectives for Polyploidy Breeding in Orchids

Bolaños-Villegas

Chen

2022

Plants

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“…Diversity 2024, 16, 218 2 of 16 While orchids can establish associations with various fungal partners, not all associations have equal impacts on orchid growth and development [10,11]. Since mycorrhizal fungi vary significantly among orchid species [12,13], thorough elucidation of the diversity and function of the OMF associated with each orchid species is vital.…”

Section: Introductionmentioning

confidence: 99%

“…This genus includes approximately 80 accepted species [14] that are native to tropical and subtropical regions from Asia to northeastern Australia [14,15]. This genus also has significant popularity in the floricultural industry, and numerous hybrid varieties are available on the market [16]. Despite their importance, several species have become endangered or rare in their natural habitats [17].…”

Section: Introductionmentioning

confidence: 99%

Mycorrhizal Fungi of Phalaenopsis japonica (Orchidaceae) and Their Role in Seed Germination and Seedling Development

Chamara,

Rammitsu,

Minobe

et al. 2024

Diversity

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Epiphytic orchids comprise 68% of vascular epiphytes globally; nevertheless, many are endangered. One such epiphytic orchid is Phalaenopsis japonica, which is widely used in the floricultural industry. This study aimed to identify the mycorrhizal fungi of adult P. japonica and their roles in seed germination and seedling development. Root samples were collected from 32 adults across 4 sites in southern Japan, and mycorrhizal fungi were identified using Sanger and high-throughput sequencing (HTS). The results show phylogenetically diverse mycobionts, mainly Ceratobasidiaceae (CE) and Tulasnellaceae (TU), with dominant OTUs designated CE6 and CE22. Sanger sequencing found 9 OTUs, 4 CE, and 5 TU; HTS detected 22 OTUs, 4 CE, 16 TU, and 2 Serendipitaceae. Seeds inoculated with CE6 improved germination and protocorm development compared with other strains. In addition, asymbiotic seedlings inoculated with CE6, CE22, and TU18 displayed varying effects in growth, with CE6 being the most notable. While TU18 did not promote seed germination, it effectively promoted leaf development in seedlings. Overall, Ceratobasidiaceae was predominantly associated with seed germination, seedling growth, and the adult stages, with CE6 and CE22 becoming the primary partners throughout the life history of P. japonica. Our findings illuminate mycorrhizal symbiosis in epiphytic habitats, offering conservation and commercial production insights.

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In vitro production of Phalaenopsis orchids

Yahya,

Killi Haworth,

Özden

et al. 2024

Turkish Journal of Biodiversity

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Phalaenopsis – known as moth orchids – are the most popular orchids cultivated indoors as decorative house plants. This makes propagation and cultivation of Phalaenopsis important for commercial growers. Enhancements to the micropropagation of Phalaenopsis would have pronounced economic benefits through reduced losses and wastage. We examined the effects of several nutrient media and specific plant growth regulators (PGRs) belonging to the gibberellic acid and cytokinin groups on the in vitro germination of Phalaenopsis seeds, utilizing a single group pretest-posttest model. The effects of several nutrient media such as: Knudson C (KCM), Lindemann (LM), Orchimax (-OM), Orchimax + activated charcoal (+OM), Murashige & Skoog (MS), as well as various PGRs such as 6-Benzylaminopurine (6BA), 6-Furfurylaminopurine (KIN), Adenin hemisulfate (AHS), Thidiazuron (TDZ), 2-Isopentenyl adenine (2iP), and Gibberellic acid (GA3), on the process of germination were also investigated. The explants obtained from the germinating seedlings were subjected to direct organogenesis, and the optimal PGR and tissue fragments were determined. The +OM medium facilitated the shortest germination period (in days). An inverse relationship between the concentration of TDZ and the percentage of germination in the context of the employed PGRs was observed. Apart from TDZ, the remaining PGRs exhibited a positive correlation with concentration. However, no significant difference in germination was observed in comparison to the control. The findings of direct organogenesis investigations revealed that the medium that exhibited the highest productivity was enriched with 5.0 ppm of 6BA. The media containing TDZ exhibited a reduced level of efficiency. Particularly, the group treated with 1.0 ppm of TDZ exhibited reduced efficacy compared to the control group. All concentrations of cytokinin in root elongation stage exhibited a favorable impact in comparison to the control. The variance between these PGRs was not statistically significant.

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The Breeding of Phalaenopsis Hybrids

Cited by 4 publications

References 60 publications

Advances and Perspectives for Polyploidy Breeding in Orchids

Advances and Perspectives for Polyploidy Breeding in Orchids

Mycorrhizal Fungi of Phalaenopsis japonica (Orchidaceae) and Their Role in Seed Germination and Seedling Development

In vitro production of Phalaenopsis orchids

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