The Breakdown of Embryonic (Chick) Cartilage and Bone Cultivated in the Presence of Complement-Sufficient Antiserum

The Role of Soft Connective Tissue in the Breakdown of Pig Articular Cartilage Cultivated in the Presence of Complement-Sufficient Antiserum to Pig Erythrocytes

“…Recently, other workers have also observed an increase in the permeability of degraded cartilage to IgG. Lachmann et al [1969] found that in embryonic chick limb bones grown in the pres ence of complement-sufficient antiserum to chicken erythrocytes, immu noglobulins could not be demonstrated in the interior of the cartilage until the breakdown of the matrix had reached an advanced stage. In 'ex perimental antigen-induced arthritis' in the rabbit, Cooke et al [1972b], using immunohistochemical methods, demonstrated immunoglobulin and C , as well as the inducing antigen (bovine serum albumin or ovalbumin) in the cartilage and in other collagenous tissues of the inflamed joint but not in normal cartilage.…”

Immunopathologic Studies in Relapsing Polychondritis

“…The 14C-protein synthesized de novo was chromatographically purified with DEAE and G-200 chromatography into specific immunoglobulin classes and this radio-labeled material utilized in three types of experiments: (a) Radio-immunodiffusion was performed in which labelled immunoglobulin and "cold" rabbit anti-human PG antibody were diffused against hyaluronidase treated PG and PP-L. Radiographic patterns were developed following 4 quently, in conjunction with a low grade temperature and malaise, episcleritis occurred followed shortly thereafter by pain and swelling of both ears with compromise of the canals due to collapse (Fig. 1).…”

“…There is little insight into etiologic or pathophysiologic factors operative in this disease. A pathologic similarity has been shown to exist between polychondritis and the experimental papain (1) and hypervitaminosis-A (2) induced depletion of cartilaginous matrix as well as the model of complement-dependent cartilage lysis in vitro, mediated by anti-cell membrane antibody (3,4). However, the inflammation with predominant round cell infiltration and degeneration of chondrocytes observed in polychondritis are conspicuous differences.…”

Cartilage antigen dependent lymphotoxin release. Immunopathological significance in articular destructive disorders.

“…There is ample evidence of the capacity of acid and neutral proteases derived from a variety of cellular sources to degrade cartilage proteoglycan, and thus perhaps phagocytosis of proteoglycan constituents themselves or in immune complex form may facilitate such release. It has been shown that antibody directed to cell membrane antigens is capable in the presence of fresh complement of inducing cartilage matrix dissolution in chick limb bud rudiments (Fell, Coombs, and Dingle, 1966;Lachmann, Coombs, Fell, and Dingle, 1969). Presumably the immune response is directed against chondrocyte surface antigenic determinants facilitating a selective release of cathepsins sufficient to induce the reversible response.…”