The Brazilian scorpion Tityus costatus Karsch: genes, peptides and function

Diego‐García, Elia; Batista, Cesar Vicente Ferreira; García‐Gómez, Blanca I.; Lucas, Sylvia; Cândido, Denise M.; Gómez-Lagunas, Froylán; Possani, Lourival D.

doi:10.1016/j.toxicon.2004.10.014

Cited by 95 publications

(55 citation statements)

References 35 publications

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“…Cabe señalar que la única banda proteica correspondiente al pico IV, no necesariamente nos indica que se trata de una sola proteína; lo más probable es que contenga varios tipos de péptidos con diversas actividades pero muy parecidos en el peso molecular. Algo muy ilustrativo al respecto es lo encontrado en otros venenos de escorpiones del género Tityus, como por ejemplo en el veneno de Tityus costatus donde se han encontrado por lo menos 84 componentes de bajo peso molecular (Diego-García et al 2005). …”

Section: Resultados Y Discusiónunclassified

“…Otros venenos que se han estudiado son los de Tityus bahiensis (Becerril et al 1996, Pimenta et al 2001, T. stigmurus (Torell, 1876), T. cambridgei Pocock, 1897, T. zulianus GonzalezSponga, 1981, T. fasciolatus Pessôa, 1935, T. discrepans (Karsch, 1879, T. trivitattus Kraepelin, 1898 y T. costatus (Karsch, 1879) (Becerril et al 1996, Batista et al 2000, 2002Murguia et al 2004, Diego-García et al 2005, Borges et al 2004, Wagner et al 2003, D´Suze et al 2004, Coronas et al 2003. En estos estudios se han purificado toxinas específicas para artrópodos (D´Suze et al 2004), insectos (Pimenta et al 2001) y mamíferos (Borges et al 1990); en algunos casos también se han encontrado toxinas con acción tanto sobre insectos como mamíferos (Pimenta et al 2001).…”

Section: Introductionunclassified

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Estudio bioquímico del veneno de Tityus kaderkai(Scorpiones: Buthidae) con notas sobre su distribución y hábitat en el Perú

2013

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Estudio bioquímico del veneno de picos proteicos (I -VII).Los ensayos de toxicidad han permitido identificar tres toxinas que afectan a Mus musculus y que se encuentran asociadas a los picos IV, V y VII; asimismo, se ha detectado toxicidad sobre Gryllus sp. en los picos IV, V, VI y VII. Entre las actividades enzimáticas ensayadas, se ha encontrado actividad proteolítica sobre caseína en el pico I y actividad de hialuronidasa en el pico IV con una actividad específica de 205.6 μg/min/mg. Tanto en el veneno soluble como en las fracciones colectadas no se encontró actividad de fosfolipasa, anticoagulante ni hemolítica. El trabajo incluye notas sobre la distribución y el hábitat de la especie.Palabras claves: veneno; escorpión; toxina; enzima; Tityus kaderkai. AbstractThe biochemistry of the venom of Tityus kaderkai Kovařik, 2005 from Madre de Dios department, has been studied. The soluble venom contains 47.6% of protein. The venom proteins were separated from 12.9 mg of venom using cationic exchange chromatography in CM Sephadex C-25 with a 0.05 M ammonium acetate buffer pH 7.0. The chromatography profiles show seven peaks of proteins (I -VII) and five protein bands were distinguished in the crude venom, by PAGE-SDS. The toxicity assays allowed the identification of three toxins affecting Mus musculus which were associated to peaks IV, V and VII. Toxic proteins to Gryllus sp. were also found associated to peaks IV, V, VI and VII. Through the enzymatic activity, the presence of proteolytic activity over casein was found related to the first peak. Hyaluronidase activity has also been found in the peak IV with a specific activity 205.6 μg/min/mg. However, the crude venom and collected fractions did not show any phospholipase, anticoagulant, nor hemolytic activity. Notes on the distribution pattern and habitat are also included.

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Section: Resultados Y Discusiónunclassified

Section: Introductionunclassified

Estudio bioquímico del veneno de Tityus kaderkai(Scorpiones: Buthidae) con notas sobre su distribución y hábitat en el Perú

2013

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“…Sequences were aligned using the program Seaview v.4.2.11 (38). Of all aligned toxins, Tcl1 and Tcl2 show remarkable sequence resemblance (87% identity) to T. serrulatus Ts1 (also known as gamma toxin) and also to gamma-like toxins Tb1 (from T. bahiensis), Tst1 (from T. stigmurus) (35) and the peptide encoded by T. costatus clone 1 (Tcogamma) (32). Identity to β-toxin Ts2 from T. serrulatus is lower (68 and 70% identity for Tcl1 and Tcl2 respectively).…”

mentioning

confidence: 99%

“…Homologs of T. serrulatus Ts1 had only been found so far in other Brazilian Tityus such as T. bahiensis, T. stigmurus and T. costatus, species belonging to the subgenus Tityus (32,35). Significantly, T. discrepans and T. zulianus, two species sympatric with T. clathratus in Venezuela, do not produce such homologs but Na v toxins with divergent N-and C-termini with respect to Ts1 (10,33 Thus far T. clathratus is the only species from the north of the Amazon Basin producing putative toxin homologs of southern Brazilian Tityus, which provides further support for the evolutionary relationship between subgenera Archaeotityus (which has a trans-Amazonian distribution) and Tityus and its separation from the subgenus Atreus as suggested previously by Borges et al (4).…”

mentioning

confidence: 99%

Scorpions from the primeval subgenus Archaeotityus produce putative homologs of Tityus serrulatus toxins active on voltage-gated sodium channels

Borges¹,

Jowers²,

Bónoli³

et al. 2012

J. Venom. Anim. Toxins incl. Trop. Dis

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It has been proposed that the subgenus Archaeotityus comprises the most ancient species group within the medically important scorpion genus Tityus. cDNA encoding sodium-channel active toxins from the type species of this subgenus, Tityus clathratus (central Venezuela), have been isolated and sequenced. Two cDNAs were retrieved that encoded 61 amino acid-long putative neurotoxins named Tcl1 and Tcl2. Sequence identity was highest (87%) when both were compared with β-toxin Ts1 from the Brazilian scorpion Tityus serrulatus and its homologs from T. bahiensis, T. stigmurus, and T. costatus. A Bayesian analysis indicated statistical support for the grouping of T. clathratus Tcl1 and Tcl2 with Brazilian gammalike β-toxins, reinforcing previous phylogenetic studies which suggested an evolutionary relationship between the subgenus Archaeotityus and scorpion species inhabiting southeast South America belonging to the subgenus Tityus.

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“…Referencias: 1 (Papp et al, 2009), 2 (Batista et al, 2002), 3 (D'Suze et al, 1999), 4 (Possani et al, 2011), 5 (Diego- Garcia et al, 2005), 6 (Liu et al, 2009), 7 (Kozminsky-Atias, Somech e Zilberberg, 2007). ⃰ Resultados obtidos nessa dissertação.…”

Section: Toxina Ts7unclassified

Novo método de fracionamento da peçonha do escorpião Tityus serrulatus e caracterização eletrofisiológica das toxinas Ts6 e Ts7

Cerni¹

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The Brazilian scorpion Tityus costatus Karsch: genes, peptides and function

Cited by 95 publications

References 35 publications

Estudio bioquímico del veneno de Tityus kaderkai(Scorpiones: Buthidae) con notas sobre su distribución y hábitat en el Perú

Estudio bioquímico del veneno de Tityus kaderkai(Scorpiones: Buthidae) con notas sobre su distribución y hábitat en el Perú

Scorpions from the primeval subgenus Archaeotityus produce putative homologs of Tityus serrulatus toxins active on voltage-gated sodium channels

Novo método de fracionamento da peçonha do escorpião Tityus serrulatus e caracterização eletrofisiológica das toxinas Ts6 e Ts7

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