The Botrytis cinerea Xylanase BcXyl1 Modulates Plant Immunity

Yang, Yuankun; Yang, Xiufen; Dong, Yuchen; Qiu, Dewen

doi:10.3389/fmicb.2018.02535

Cited by 55 publications

(67 citation statements)

References 56 publications

(83 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For instance, ppxyn1 and ppxyn2 have been shown to play a crucial role in the infection process of P. parasitica [25]. In B. cinerea, BcXyl1, acting as an effector, is able to induce plant defense responses and contributes to pathogenicity [44]. In the current research, RcXYN1-RcXYN4, four xylanases in R. cerealis, were predicted to be secreted proteins, implying their potential as a necrotrophic effector.…”

Section: Discussionmentioning

confidence: 62%

Global Characterization of GH10 Family Xylanase Genes in Rhizoctonia cerealis and Functional Analysis of Xylanase RcXYN1 During Fungus Infection in Wheat

Lü

Liu

Zhang

2020

IJMS

View full text Add to dashboard Cite

Wheat (Triticum aestivum L.) is an important staple crop. Rhizoctonia cerealis is the causal agent of diseases that are devastating to cereal crops, including wheat. Xylanases play an important role in pathogenic infection, but little is known about xylanases in R. cerealis. Herein, we identified nine xylanase-encoding genes from the R. cerealis genome, named RcXYN1–RcXYN9, examined their expression patterns, and investigated the pathogenicity role of RcXYN1. RcXYN1–RcXYN9 proteins contain two conserved glutamate residues within the active motif in the glycoside hydrolase 10 (GH10) domain. Of them, RcXYN1–RcXYN4 are predicted to be secreted proteins. RcXYN1–RcXYN9 displayed different expression patterns during the infection process of wheat, and RcXYN1, RcXYN2, RcXYN5, and RcXYN9 were expressed highly across all the tested inoculation points. Functional dissection indicated that the RcXYN1 protein was able to induce necrosis/cell-death and H2O2 generation when infiltrated into wheat and Nicotiana benthamiana leaves. Furthermore, application of RcXYN1 protein followed by R. cerealis led to significantly higher levels of the disease in wheat leaves than application of the fungus alone. These results demonstrate that RcXYN1 acts as a pathogenicity factor during R. cerealis infection in wheat. This is the first investigation of xylanase genes in R. cerealis, providing novel insights into the pathogenesis mechanisms of R. cerealis.

show abstract

Section: Discussionmentioning

confidence: 62%

Global Characterization of GH10 Family Xylanase Genes in Rhizoctonia cerealis and Functional Analysis of Xylanase RcXYN1 During Fungus Infection in Wheat

Lü

Liu

Zhang

2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Besides, a SGNH hydrolase subfamily protein BcXyl1 was identified to induce cell death in N. benthamiana, tomato, soybean and cotton (Yang et al, 2018c). BcXyl1 exhibited xylanase activity, but its cell death inducing activity was independent of the enzymatic activity.…”

Section: Cell Wall-degrading Enzyme Family Cdipsmentioning

confidence: 99%

Apoplastic Cell Death-Inducing Proteins of Filamentous Plant Pathogens: Roles in Plant-Pathogen Interactions

Han

et al. 2020

Front. Genet.

View full text Add to dashboard Cite

Filamentous pathogens, such as phytopathogenic oomycetes and fungi, secrete a remarkable diversity of apoplastic effector proteins to facilitate infection, many of which are able to induce cell death in plants. Over the past decades, over 177 apoplastic cell death-inducing proteins (CDIPs) have been identified in filamentous oomycetes and fungi. An emerging number of studies have demonstrated the role of many apoplastic CDIPs as essential virulence factors. At the same time, apoplastic CDIPs have been documented to be recognized by plant cells as pathogen-associated molecular patterns (PAMPs). The recent findings of extracellular recognition of apoplastic CDIPs by plant leucine-rich repeat-receptor-like proteins (LRR-RLPs) have greatly advanced our understanding of how plants detect them and mount a defense response. This review summarizes the latest advances in identifying apoplastic CDIPs of plant pathogenic oomycetes and fungi, and our current understanding of the dual roles of apoplastic CDIPs in plant-filamentous pathogen interactions.

show abstract

“…As a natural barrier, the plant cell wall provides mechanical strength and rigidity in order to prevent invasion by pathogens. To destroy the plant cell wall and successfully colonize host plants, B. cinerea secretes a diverse array of metabolites and proteins [24,26,27]. These proteins belong to cell wall-degrading enzyme (CWDE) families and include pectinases, xylanases, and endo-PGs; they are usually considered to be important virulence factors and act via host tissue impregnation and macromolecule degradation [28].…”

Section: Transcriptional Analysis Of Genes Encoding Putative Secretedmentioning

confidence: 99%

Genome-wide Transcriptomic Analysis of the Response ofBotrytis cinereato Wuyiencin

Shi

Liu

Wei

et al. 2019

Preprint

View full text Add to dashboard Cite

AbstractGrey mould is caused by the ascomycetes Botrytis cinerea in a range of crop hosts. As a biological control agent, the nucleoside antibiotic wuyiencin has been industrially produced and widely used as an effective fungicide. To elucidate the effects of wuyiencin on the transcriptional regulation in B. cinerea, we, for the first time, report a genome-wide transcriptomic analysis of B. cinerea treated with wuyiencin. We could identify 2067 differentially expressed genes (DEGs); of them, 886 and 1181 genes were significantly upregulated and downregulated, respectively. Functional categorization indicated that genes involved in amino acid metabolism and those encoding putative secreted proteins were remarkably influenced in response to wuyiencin treatment. Moreover, the expression of genes involved in protein synthesis and energy metabolism (oxidative phosphorylation) and of those encoding ATP-binding cassette transporters was markedly upregulated, whereas that of genes participating in DNA replication, cell cycle, and stress response was downregulated. Furthermore, wuyiencin resulted in mycelial malformation and negatively influenced cell growth rate and conidial yield in B. cinerea. Our results suggest that this nucleoside antibiotic regulates all aspects of cell growth and differentiation in B. cinerea. To summarize, we identified candidate pathways and target genes that may offer insights into the protective and antagonistic mechanisms underlying the action of biological control agents.

show abstract

The Botrytis cinerea Xylanase BcXyl1 Modulates Plant Immunity

Cited by 55 publications

References 56 publications

Global Characterization of GH10 Family Xylanase Genes in Rhizoctonia cerealis and Functional Analysis of Xylanase RcXYN1 During Fungus Infection in Wheat

Global Characterization of GH10 Family Xylanase Genes in Rhizoctonia cerealis and Functional Analysis of Xylanase RcXYN1 During Fungus Infection in Wheat

Apoplastic Cell Death-Inducing Proteins of Filamentous Plant Pathogens: Roles in Plant-Pathogen Interactions

Genome-wide Transcriptomic Analysis of the Response ofBotrytis cinereato Wuyiencin

Contact Info

Product

Resources

About