The Bladder Acellular Matrix Graft in a Rat Chemical Cystitis Model: Functional and Histologic Evaluation

Çayan, Selahíttín; Chermansky, Christopher; Schlote, Norbert; Sekido, Noritoshi; Nunes, Lora; Dahiya, Rajvir; Tanagho, Emil A.

doi:10.1016/s0022-5347(05)64746-5

Cited by 46 publications

(31 citation statements)

References 20 publications

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“…ac.jp valve, 9,10 skin, 11,12 bladder, 13 and urethra. 14 Decellularization techniques have been developed to reduce the immunogenicity or calcification of biomaterials after implantation.…”

Section: Heartmentioning

confidence: 99%

“…Each protocol has its advantages and disadvantages, and an optimal protocol depends on the type of tissue. 9,13,18 In the present study, based on the aforementioned evidence and experiments, we investigated the effect of decellularization protocols on the DNA content and the histology of tissue as well as the effect of decellularized esophagus on esophageal epithelial cell differentiation.…”

Section: Heartmentioning

confidence: 99%

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Evaluation of decellularized esophagus as a scaffold for cultured esophageal epithelial cells

Ozeki

Narita

Kagami

et al. 2006

J Biomedical Materials Res

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Recently, decellularized tissue has been reported to have the potential to regenerate a variety of tissues. However, the optimal protocol for a decellularized esophagus has not been studied. Here, we investigated the effect of different decellularization protocols on the histology and biocompatibility of decellularized esophagi in view of future applications to tissue engineering. The esophageal mucosal epithelium (EP) from 4-week-old Wistar rats was enzymatically dissociated and cultured with growth-arrested feeder cells. Two methods for decellularization using deoxycholic acid (DEOX) or Triton X-100 (TRITON) were compared on esophagi from adult Wistar rats. Those treated with DEOX showed superior mechanical properties, maintenance of extracellular matrix, and lower DNA content than those treated with TRITON. To evaluate the biocompatibility of the scaffold, cultured (passage 3) esophageal epithelial cells were seeded inside the decellularized esophagus and cultured for 7 days. The cells seeded onto the decellularized esophagus were examined histologically and immunocytochemically. Esophageal epithelial cells were stratified into three to four cellular layers in vitro inside the decellularized esophagus, to show polarity. The results from immunocytochemistry indicated that the seeded epithelial cells expressed characteristic marker proteins for native esophageal EP. Decellularized esophagus showed suitable compatibility as a scaffold material for esophageal tissue engineering.

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Section: Heartmentioning

confidence: 99%

Section: Heartmentioning

confidence: 99%

Evaluation of decellularized esophagus as a scaffold for cultured esophageal epithelial cells

Ozeki

Narita

Kagami

et al. 2006

J Biomedical Materials Res

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“…Urodynamic evaluations were performed at the end of treatment for each group of rats as described previously (Cayan et al 2002(Cayan et al , 2003(Cayan et al , 2006. All rats were anesthetized by intraperitoneal injection with 30 mg/kg mebumalnatrium.…”

Section: Functional Evaluationmentioning

confidence: 99%

“…To determine the functional changes following by bladder fibrosis in orchiectomized rats, urodynamic evaluations were performed at the end of treatment for each group of rats as described previously (Cayan et al 2002(Cayan et al , 2003(Cayan et al , 2006. The maximal bladder volume and bladder compliance were determined.…”

Section: Androgen Deprivation Induced Bladder Fibrosis Causes Bladdermentioning

confidence: 99%

Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-.BETA. in Orchiectomized Mature Rats

Zhang

Ji-min

et al. 2012

Tohoku J. Exp. Med.

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Symptomatic late-onset hypogonadism, one of the most common elder diseases, is defined as a syndrome associated with a deficiency in serum testosterone. Recent studies have indicated that androgen deficiency in men is also associated with lower urinary tract symptoms and bladder dysfunction. To determine the pathologic consequences of androgen deprivation in bladder histology and function, we addressed the underlying mechanism. Male rats were divided into 4 groups: emasculated rats (EMR), emasculated rats treated with testosterone, emasculated rats treated with anti-transforming growth factor-β (TGF-β) neutralizing antibody, and sham surgery rats. TGF-β is a common profibrotic factor that mediates the pathologic process of fibrosis in multiple organs. Two months later, urodynamic evaluations were employed to determine the bladder function in vivo. And then rats were sacrificed, and the bladder tissues were collected. Histological studies were employed to determine the degree of bladder fibrosis. Real time PCR was used to evaluate the mRNA level of pro-collagen I, a fibrotic marker. We demonstrate here that androgen deficiency induces bladder fibrosis and decreases the bladder maximal volume and compliance. Androgen replacement treatment completely prevented the histological and functional abnormalities induced by androgen deficiency. Subsequently, we identified that androgen deprivation induced the induction of TGF-β mRNA level. Importantly, treatment with anti-TGF-β antibody abolished androgen deprivation-induced bladder fibrosis and dysfunction. Our study reveals an essential role of TGF-β in the pathogenesis of androgen deprivation-induced bladder fibrosis and dysfunction and offers a potential target for prevention and treatment of bladder dysfunction associated with androgen deficiency.

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“…To render the canine ureteral matrices decellular, they were excised from the beagles and treated with deoxycholic acid, as reported previously [9]. Briefly, the ureters were shaken in 10 ml of 9.1 mM PBS containing 0.1% sodium azide for 12 hours in a humidified 5% CO 2 atmosphere at 37°C.…”

Section: Generation Of Udmmentioning

confidence: 99%

Ureter

Ueda

2011

Applied Tissue Engineering

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The Bladder Acellular Matrix Graft in a Rat Chemical Cystitis Model: Functional and Histologic Evaluation

Abstract: In this rat chemical cystitis model bladder augmentation with a bladder acellular matrix graft led to functional and histological improvement over diseased host bladder.

Cited by 46 publications

References 20 publications

Evaluation of decellularized esophagus as a scaffold for cultured esophageal epithelial cells

Evaluation of decellularized esophagus as a scaffold for cultured esophageal epithelial cells

Androgen Deprivation Induces Bladder Histological Abnormalities and Dysfunction via TGF-.BETA. in Orchiectomized Mature Rats

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