The biosynthesis and functionality of the cell-wall of lactic acid bacteria

Delcour, Jean; Ferain, Thierry; Deghorain, Marie; Palumbo, Emmanuelle; Hols, Pascal

doi:10.1007/978-94-017-2027-4_7

Cited by 123 publications

(211 citation statements)

References 156 publications

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“…cell wall protein, S-layer, teichoic acid, polysaccharides) or the inner plasma membrane (i.e. Lipotechoic acids) [42]. Unlike Gram positives, the cell wall of Gram negative bacteria consists of a thinner peptidoglycan layer (≈10nm), which is topped by an outer membrane consisting of proteins (i.e.…”

Section: Bacterial Characteristicsmentioning

confidence: 99%

The role of cell-surface interactions in bacterial initial adhesion and consequent biofilm formation on nanofiltration/reverse osmosis membranes

Habimana

Semiao

Casey

2014

Journal of Membrane Science

231

113

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Section: Bacterial Characteristicsmentioning

confidence: 99%

The role of cell-surface interactions in bacterial initial adhesion and consequent biofilm formation on nanofiltration/reverse osmosis membranes

Habimana

Semiao

Casey

2014

Journal of Membrane Science

231

113

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“…One milliliter of dH 2 O was added to the pellet. After vortexing, the sample was boiled at 100°C for 15 minutes by placing in water bath.…”

Section: P1 Protocolmentioning

confidence: 99%

“…The murein layer (a thick peptidoglycan layer) is the ubiquitous component of the Gram-positive cell wall which provides shape, stability and viability. This layer contains almost equal amounts of polysaccharides and peptides (1,2) and is composed of a polymer of disaccharide (glycan) chains of repeating N-acetylglucosamine and N-acetylmuramic acid residues (linked β1 → 4) and is cross-linked by short chains of amino acids (peptide) (1).…”

Section: Introductionmentioning

confidence: 99%

An Efficient DNA Extraction Method for Lactobacillus casei, a Difficult-to-Lyse Bacterium

Alimolaei¹,

Golchin²

2016

Int J Enteric Pathog

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Background: There are several protocols to extract DNA from Lactobacillus spp. In the case of L. casei it is harder because of its especial and thick cell wall. Objectives: In this study, nine DNA extraction protocols (by lysozyme treatment) were evaluated and compared in two categories (traditional and kit-based protocols) and an improved method was presented. Materials and Methods: DNA quantity and quality was determined by spectrophotometry, agarose gel electrophoresis and polymerase chain reaction (PCR). Results:The results revealed that the yield of extracted DNA differed by each protocol (5.8 -17.1 μg/100 μL), but provided appropriate DNA for PCR amplification. The modified protocol offered the best total DNA extraction method when both quality (DNA purity; 1.54 μg) and quantity (DNA yield; 17.1 μg) were considered. Conclusions: We suggest this protocol for effective and inexpensive DNA isolation from L. casei for downstream biological processes such as PCR.

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“…Their cell wall is made up of peptidoglycan, teichoic, and lipoteichoic acid structures, an S-layer, in some species, and neutral polysaccharides. The teichoic acid structures, the cell wall polysaccharides, and the exopolysaccharides allow adhesion to macromolecules [7]. The aim of this study was to isolate and identify heavy metals resistant probiotics, mainly lactic acid bacteria, from three different metal polluted soils in Mauritius and to determine the absorbing or binding potential of the isolates to four different heavy metals, namely, Cd, Cr, Pb, and Hg.…”

Section: Introductionmentioning

confidence: 99%

Bioremediation of heavy metals from aquatic environment through microbial processes: A potential role for probiotics?

2017

J App Biol Biotech

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Heavy metals can cause disastrous effects on any living organisms. Probiotics have the ability to reduce metal toxicity. Bioremediation of polluted waters using these bacteria could be an alternative to conventional remediation methods. The aim of this study was to isolate and characterize heavy metal resistant probiotics. Soil samples were collected for the isolation of probiotics. Morphological, biochemical, and molecular characterization was performed for the isolates. In addition, a preliminary heavy metal minimum inhibitory concentration test was done followed by atomic absorption spectrometry (AAS) analysis analysis. Four Enterococci (BT1, BT2, MC1, and MC2) and Bacillus acidiproducens (SM1 and SM2) were isolated. Moreover, all the isolates demonstrated probiotic characteristics. BT1 and BT2 were able to tolerate mercury (Hg), cadmium (Cd), lead (Pb), and chromium (Cr) but they demonstrated poor Hg removal abilities (0.75-1.42%). MC1 and MC2 isolates could grow in medium supplemented with Cd, Pb, and Cr, respectively. MC1 showed the highest level of Pb removal (43.00% ± 0.776%) and Cd removal (46.19% ± 7.651%) from broth media. Yet, SM1 and SM2 isolates tolerated only Pb and Cr. SM2 had the ability to remove the highest amount of Cr (43.06% ± 7.991%). These reasonable heavy metal removal abilities could be further studied for efficient use in bioremediation.

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The biosynthesis and functionality of the cell-wall of lactic acid bacteria

Cited by 123 publications

References 156 publications

The role of cell-surface interactions in bacterial initial adhesion and consequent biofilm formation on nanofiltration/reverse osmosis membranes

The role of cell-surface interactions in bacterial initial adhesion and consequent biofilm formation on nanofiltration/reverse osmosis membranes

An Efficient DNA Extraction Method for Lactobacillus casei, a Difficult-to-Lyse Bacterium

Bioremediation of heavy metals from aquatic environment through microbial processes: A potential role for probiotics?

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