The biomechanical and histological effects of posterior cruciate ligament rupture on the medial tibial plateau

Deng, Zhenhan; Li, Yusheng; Lin, Zhenghua; Zhu, Yong; Zhao, Ruibo

doi:10.1186/s13018-017-0551-x

Cited by 9 publications

(6 citation statements)

References 21 publications

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“…Rabbits were sacrificed 8 weeks after operation. Biopsies of the cartilage and bone were acquired from the tibia plateau, including the loading zone and the margin zone as far as possible ( Deng et al, 2017 ). Specimens were examined by Micro-CT. Articular cartilage was removed from the articular surface by sterile scalpel and was transferred to 4°C sterile DMEM (Hyclone, United States).…”

Section: Methodsmentioning

confidence: 99%

Protective Effect of SIRT1 Activator on the Knee With Osteoarthritis

et al. 2021

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Osteoarthritis (OA), one of the most common chronic musculoskeletal disorders, is deemed to be correlated with aging. The SIRT1 activator, resveratrol, acts as a crucial regulator of aging and may have a potential therapeutic effect on OA. Rabbit OA models were established through destabilized medial meniscus surgery. A total of 40 healthy male New Zealand rabbits were divided into five groups: control group (sham operation), OA group, as well as low dose (LD), middle dose (MD), and high dose (HD) resveratrol-treated OA groups. 6 weeks after operation, 0.8 ml of normal saline was injected into the knee joints every other day in the control and OA groups, and 0.8 ml of 5, 10, and 15 μmol/L resveratrol was injected into the knee joints every other day in the LD, MD, and HD group, respectively. The rabbits were sacrificed 2 weeks after medication, and the articular cartilage of the knee joint was collected for Micro-CT, histology and Western blot analysis. Obvious articular cartilage lesion and joint space narrowing were detected in the OA group. Compared with the OA group, less osteoarthritic changes were observed in the MD and HD groups. The MD and HD groups had significantly lower bone volume fraction, trabecular number and Mankin scores than the LD and OA groups (p < 0.05). No significant difference was found between the OA and LD groups (p > 0.05). The expressions of SIRT1 and p53 detected by western blot were consistent with the aforementioned findings. Therefore, resveratrol can activate the SIRT1 gene to play a protective role in the OA process by inhibiting chondrocyte apoptosis, trabecular bone number increasing of the subchondral bone, as well as elevation of bone density. It demonstrated the importance of SIRT1 in maintaining articular cartilage health and provided a promising therapeutic intervention in the treatment of OA.

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Section: Methodsmentioning

confidence: 99%

Protective Effect of SIRT1 Activator on the Knee With Osteoarthritis

et al. 2021

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“…The animals had free access to a normal diet and fresh tap water. Surgical transection of the PCL was performed randomly to one knee and PCL of the contralateral side was exposed but not transacted [16,17]. Specifically, the rabbits were anesthetized via the intraperitoneal administration of 3% sodium pentobarbital (0.03 mg/kg) and fixed on the operating table in a supine position.…”

Section: Methodsmentioning

confidence: 99%

The morphology and histology study on rabbit degenerated medial meniscus after posterior cruciate ligament rupture

et al. 2019

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The morphology and histology changes in the medial meniscus after posterior cruciate ligament (PCL) rupture are poorly understood. Forty-eight rabbits were divided into matched mode pairs; each rabbit had an experimental side, in which the PCL was transacted, and a control side. At the 4, 8, 16 and 24 weeks after the PCL transection, each of the 12 rabbits was killed. Histology was performed to detect the expression of the tissue inhibitors of metalloproteinases-1 (TIMP-1), matrix metalloproteinase (MMP)-1 and MMP-13 in the medial meniscus. We found that medial meniscus displayed significant degenerative characteristics in morphology. The histological evaluation of the degeneration found that the expression levels of TIMP-1, MMP-1 and MMP-13 in the medial meniscus were higher in the experiment side than those in the control side (P<0.05). The expression of both TIMP-1 and MMP-13 was initially elevated and then decreased. The MMP-1 expression reached its peak swiftly and then maintained a relatively high level. There were clear time-dependent degenerative changes in the histology of the medial meniscus after PCL rupture. The high expression of TIMP-1, MMP-1 and MMP-13 in the cartilage may be responsible for the degeneration, and PCL rupture may trigger meniscus degradation and ultimately osteoarthritis.

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“…The method used was the same as that in our previous study [15,16]. In short, rabbits were injected with 3% pentobarbital solution (0.03 mg/kg) through the ear vein.…”

Section: Model Preparationmentioning

confidence: 99%

“…Petrigliano et al [12] reported that 96.5% of PCL injuries were associated with other ligament injuries, including anterior cruciate ligament, lateral collateral ligament, or lateral stable structure injury. The biomechanical or histological effects of PCL rupture on the medial femoral condyle [13], the lateral femoral condyle [14], the medical tibial plateau [15], and the medical meniscus [16] have been studied by scholars. However, the effect of PCL rupture on the MCL is still lacking, and most of the existing studies focus on the ACL.…”

Section: Introductionmentioning

confidence: 99%

The effects of posterior cruciate ligament rupture on the biomechanical and histological characteristics of the medial collateral ligament: an animal study

Xie

Dong-sheng

et al. 2021

J Orthop Surg Res

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Background To investigate the effect of complete rupture of the posterior cruciate ligament (PCL) on the biomechanics and histology of the medial collateral ligament (MCL). Materials and methods Seventy-two male rabbits were randomly divided into two groups: the ruptured group was treated with complete PCL amputation, while the intact group was only subjected to PCL exposure without amputation. Eighteen rabbits were randomly sacrificed at 8, 16, 24, and 40 weeks after the operation, and their specimens were processed for mechanical tensile testing, nano-indentation experiments, hematoxylin-eosin (HE) staining, and picrosirius-polarization staining. Results There was no significant difference in the length and maximum displacement of the MCL between the ruptured group and the intact group at each time point. The maximum load of the ruptured group was significantly smaller than that of the intact group at 40 W. The elastic modulus and micro-hardness of the ruptured group increased significantly at 24 W and decreased significantly at 40 W. At 16 W and 24 W after PCL rupture, the number of type I collagen fibers and type III collagen fibers in the MCL of the ruptured group was significantly increased compared with that of the intact group. While the type I collagen fibers of the ruptured group were significantly decreased compared with the intact group at 40 W, there was no significant difference in type III collagen fibers between the ruptured group and the intact group. Conclusion PCL rupture has no significant effect on the mechanical and histological properties of MCL in a short period of time under physiological loading, but the histological and mechanical properties of MCL decrease with time.

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The biomechanical and histological effects of posterior cruciate ligament rupture on the medial tibial plateau

Cited by 9 publications

References 21 publications

Protective Effect of SIRT1 Activator on the Knee With Osteoarthritis

Protective Effect of SIRT1 Activator on the Knee With Osteoarthritis

The morphology and histology study on rabbit degenerated medial meniscus after posterior cruciate ligament rupture

The effects of posterior cruciate ligament rupture on the biomechanical and histological characteristics of the medial collateral ligament: an animal study

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