The biology of hernia formation

Jansen, Petra Lynen; Mertens, Peter R.; Klinge, U.; Schumpelick, Volker

doi:10.1016/j.surg.2004.01.004

Cited by 124 publications

(79 citation statements)

References 14 publications

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“…When appropriate, the ProteoExtract subcellular proteome extraction kit (Calbiochem) was used to obtain cytosolic, membrane/organelle, nuclear, and cytoskeletal protein fractions. 6 mmol/L Tris-HCl (pH 6.8 at 25°C), 6% w/v SDS, 30% glycerol, 150 mmol/L DTT, 0.03% w/v bromophenol blue] were added, and beads were denatured (10 minutes, 100°C). Aliquots of the supernatant were run on SDS-PAGE gels and immunoblotted, as described.…”

Section: Adhesion Assaymentioning

confidence: 99%

Apoptosis-Like Cell Death Induction and Aberrant Fibroblast Properties in Human Incisional Hernia Fascia

Díaz

Quiles

Guillem-Marti

et al. 2011

The American Journal of Pathology

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Incisional hernia often occurs following laparotomy and can be a source of serious problems. Although there is evidence that a biological cause may underlie its development, the mechanistic link between the local tissue microenvironment and tissue rupture is lacking. In this study, we used matched tissue-based and in vitro primary cell culture systems to examine the possible involvement of fascia fibroblasts in incisional hernia pathogenesis. Fascia biopsies were collected at surgery from incisional hernia patients and non-incisional hernia controls. Tissue samples were analyzed by histology and immunoblotting methods. Fascia primary fibroblast cultures were assessed at morphological, ultrastructural, and functional levels. We document tissue and fibroblast loss coupled to caspase-3 activation and induction of apoptosis-like cell-death mechanisms in incisional hernia fascia. Alterations in cytoskeleton organization and solubility were also observed. Incisional hernia fibroblasts showed a consistent phenotype throughout early passages in vitro, which was characterized by significantly enhanced cell proliferation and migration, reduced adhesion, and altered cytoskeleton properties, as compared to non-incisional hernia fibroblasts. Moreover, incisional hernia fibroblasts displayed morphological and ultrastructural alterations compatible with autophagic processes or lysosomal dysfunction, together with enhanced sensitivity to proapoptotic challenges. Overall, these data suggest an ongoing complex interplay of cell death induction, aberrant fibroblast function, and tissue loss in incisional hernia fascia, which may significantly contribute to altered matrix maintenance and tissue rupture in vivo.

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Section: Adhesion Assaymentioning

confidence: 99%

Apoptosis-Like Cell Death Induction and Aberrant Fibroblast Properties in Human Incisional Hernia Fascia

Díaz

Quiles

Guillem-Marti

et al. 2011

The American Journal of Pathology

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“…The aetiology and pathogenesis of abdominal wall hernia formation is complex and multifactorial 1 . Evidence suggests that hernia formation and recurrence are linked to abnormal metabolism of connective tissue 2 -4 , but the pathophysiological mechanisms have yet to be described fully.…”

Section: Introductionmentioning

confidence: 99%

Connective tissue alteration in abdominal wall hernia

Henriksen

Yadete

Sørensen

et al. 2010

British Journal of Surgery

131

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“…In accordance with this, connective tissue alterations have been reported to be more pronounced in patients with a medial inguinal hernia than those with a lateral inguinal hernia (72). Hernia formation has been related to increased elasticity of the transversalis fascia (73) and weak collagen structure and/or defects in collagen (74)(75)(76) and elastic fibre (77) metabolism. However, we are not aware of any studies that have related mechanical exposures to pathologic connective tissue alterations in the inguinal region.…”

Section: Contributory Evidence With Respect To Risk Of Hernia Formationmentioning

confidence: 70%

Risk and prognosis of inguinal hernia in relation to occupational mechanical exposures - a systematic review of the epidemiologic evidence

Svendsen¹,

Frost²,

Vad³

et al. 2012

Scand J Work Environ Health

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Risk and prognosis of inguinal hernia in relation to occupational mechanical exposures -a systematic review of the epidemiologic evidence by Svendsen SW, Frost P, Vad MV, Andersen JH -One in four men undergoes inguinal hernia repair that carries a risk of recurrence and persistent pain. -Occupational mechanical exposures may increase the risk of occurrence and of an unfavourable prognosis. -The epidemiologic evidence is too limited to draw conclusions for or against any causal or prognostic associations. -There is a need for new knowledge with an emphasis on prevention. Scand J Work Environ Health. 2013;39(1):5-26. doi:10.5271/sjweh.3305 Risk and prognosis of inguinal hernia in relation to occupational mechanical exposures -a systematic review of the epidemiologic evidence Objectives The aim of this review was to evaluate the epidemiologic evidence for (i) a causal effect of occupational mechanical exposures on incidence of inguinal hernia, and (ii) a prognostic effect of such exposures on hernia recurrence and persistent pain after inguinal hernia repair. Affiliation MethodsWe performed a literature search in Medline, Embase, and Web of Science up to 3 November 2011.Central information was extracted from included studies, and strengths and limitations were discussed. ResultsAll 23 included studies focused on effects of (work) activities that hardly reflected specific occupational risk factors. Eight studies provided information on risk by occupation or occupational activities. Increased risk was reported in six studies, but inflationary bias was likely. The negative findings in two studies might well be explained by bias towards the null due to crude exposure and/or outcome assessment. Three studies on single strenuous events primarily reflected patients' beliefs regarding risk factors. Information on prognosis with respect to recurrence was found in seven studies. The studies used crude exposure assessment, and two were also underpowered. Four suggested an increased risk. Six studies on prognosis with respect to persistent pain (one of which also concerned recurrence) were practically non-informative for the purpose of this review. ConclusionsThere is insufficient epidemiologic evidence to draw meaningful conclusions about (i) the existence of causal associations between specific occupational mechanical exposures and the development of inguinal hernia, and (ii) the influence of these exposures on prognosis after inguinal hernia repair with respect to hernia recurrence and persistent pain.

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The biology of hernia formation

Cited by 124 publications

References 14 publications

Apoptosis-Like Cell Death Induction and Aberrant Fibroblast Properties in Human Incisional Hernia Fascia

Apoptosis-Like Cell Death Induction and Aberrant Fibroblast Properties in Human Incisional Hernia Fascia

Connective tissue alteration in abdominal wall hernia

Risk and prognosis of inguinal hernia in relation to occupational mechanical exposures - a systematic review of the epidemiologic evidence

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