The biological effects of 632.8-nm low energy He–Ne laser on peripheral blood mononuclear cells in vitro

Gülsoy, Murat; Ozer, Gokmen Hursit; Bozkulak, Ozguncem; Tabakoglu, Ozgur; Aktaş, Esin; Deniz, Günnur; Ertan, Cemile

doi:10.1016/j.jphotobiol.2005.11.004

Cited by 48 publications

(24 citation statements)

References 13 publications

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“…In human peripheral blood mononuclear cells (PBMC), another group reported in two papers that interleukin-1 alpha (IL-1 alpha), tumor necrosis factor-alpha (TNF-alpha), interleukin-2 (IL-2), and interferon-gamma (IFN-gamma) at a protein and gene level in PBMC was increased after He-Ne irradiation at 18.9 J/cm 2 and decreased with 37.8 J/cm 2 [59,60]. Stimulation of human PBMC proliferation and murine splenic lymphocytes was also reported with He-Ne LLL [61,62]. In terms of innate immune cells, enhanced phagocytic activity of murine macrophages have been reported with energy densities ranging from 100 to 600 J/cm 2 , with an optimal dose of 200 J/cm 2 [63].…”

Section: In Vitro Studies Of Lllmentioning

confidence: 99%

Lasers, stem cells, and COPD

et al. 2010

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The medical use of low level laser (LLL) irradiation has been occurring for decades, primarily in the area of tissue healing and inflammatory conditions. Despite little mechanistic knowledge, the concept of a non-invasive, non-thermal intervention that has the potential to modulate regenerative processes is worthy of attention when searching for novel methods of augmenting stem cell-based therapies. Here we discuss the use of LLL irradiation as a "photoceutical" for enhancing production of stem cell growth/chemoattractant factors, stimulation of angiogenesis, and directly augmenting proliferation of stem cells. The combination of LLL together with allogeneic and autologous stem cells, as well as post-mobilization directing of stem cells will be discussed.

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Section: In Vitro Studies Of Lllmentioning

confidence: 99%

Lasers, stem cells, and COPD

et al. 2010

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“…A possible explanation is that the effects of laser therapy occur by transforming light energy into chemistry and propagating their effects to the tissues and surrounding areas. The absorbed energy can act in two ways: (i) stimulating the release of autacoid substances (histamine, serotonin and bradykinin) and (ii) modifying the normal enzymatic reactions, both in the sense of excitement as inhibition [41]. …”

Section: Discussionmentioning

confidence: 99%

“…There are some indications that the phototherapy can reduce reactive oxygen species (ROS), while antioxidant levels increase [46]. Moreover, as mentioned earlier, laser therapy can stimulate the respiratory chain and the synthesis of ATP [41]. These effects, in turn, also contribute to a decrease in CK activity and the reduction of blood lactate accumulation leading to recovery from muscle fatigue [32,46-50].…”

Section: Discussionmentioning

confidence: 99%

Effects of light-emitting diodes on muscle fatigue and exercise tolerance in patients with COPD: study protocol for a randomized controlled trial

Miranda¹,

Leal-Junior²,

Marchetti³

et al. 2013

Trials

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BackgroundLight-emitting diodes (LED) have been used to minimize muscle fatigue in athletes and healthy subjects. Patients with chronic obstructive pulmonary disease (COPD) are susceptible to early muscle fatigue.ObjectiveThe objective of this study is to investigate the acute effects of LED on muscle function, exercise capacity and cardiorespiratory responses during isometric and dynamic exercise in patients with COPD.MethodsThis study will assess 30 patients with moderate to severe obstruction (forced expiratory volume-one second,FEV1 ≤70% predicted). Isometric and dynamic protocols will be conducted in two visits each, for a total of four visits a week apart. First, venous blood will be taken from the patients. The isometric protocol will start with the determination of the maximum voluntary isometric contraction (MIVC) to determine the workload (60% of MIVC) for the isometric endurance test (IET). Patients will be randomized to receive either the placebo or LED application (each point will be irradiated for 30 s and the energy received at each point will be 41.7 J). Immediately after finishing this procedure, the patients will carry out the IET until the limit of tolerance or until a 20% fall of strength is observed. After the test, another blood draw will be taken. In another visit (one week later), the same order of procedures will be performed, except with the opposite (LED or placebo). For the dynamic endurance test (DET), the same procedures described above will be followed, except with 75% of the maximal workload obtained from the incremental cycle ergometer test used instead of the IET. The electromyography will be recorded during the isometric and dynamic protocols. Differences in muscle function, exercise capacity and cardiorespiratory responses between the LED and placebo applications will be analyzed. The therapeutic effects of LED could minimize muscle fatigue in patients with COPD by increasing exercise tolerance.Trial registrationTrial registration number: NCT01448564

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“…Morphological changes in the mitochondria of lymphocytes were also observed after radiation with red laser, as well as the proliferation of mononuclear cells, responses that might be beneficial in the process of tissue repair (Gulsoy et al, 2006;Karu, 1992).…”

Section: Therapeutic Modalitiesmentioning

confidence: 99%