The biological characteristics of the canine adenovirus type 1 from fox and the transcriptome analysis of the infected MDCK cell

Zhu, Yanzhu; Sun, Jie; Yan, Minghao; Lian, Shizhen; Hu, Bo; Liu, Shuang; Li, Yali; Zhang, Yufei; Yan, Xijun

doi:10.1002/cbin.11537

Cited by 6 publications

(5 citation statements)

References 34 publications

(39 reference statements)

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“…All sequences are given in Table 1 . Nucleotide and predicted amino acid sequences were compiled and edited using the BioEdit (version 7.2) program and aligned using the MegAlign software (version 17.1) from ClustalW [ 16 ]. Phylogenetic analysis of nucleotide sequences (nt) and protein gene dataset was performed using MEGA version 6.0 maximum compound likelihood nucleotide substitution model.…”

Section: Methodsmentioning

confidence: 99%

Molecular Detection and Genetic Characterization of Japanese Encephalitis Virus in Animals from 11 Provinces in China

Zhao

Gao

Shi

et al. 2023

Viruses

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Japanese encephalitis virus (JEV), which uses a mosquito primary vector and swine as a reservoir host, poses a significant risk to human and animal health. JEV can be detected in cattle, goats and dogs. A molecular epidemiological survey of JEV was conducted in 3105 mammals from five species, swine, fox, racoon dog, yak and goat, and 17,300 mosquitoes from 11 Chinese provinces. JEV was detected in pigs from Heilongjiang (12/328, 3.66%), Jilin (17/642, 2.65%), Shandong (14/832, 1.68%), Guangxi (8/278, 2.88%) and Inner Mongolia (9/952, 0.94%); in goats (1/51, 1.96%) from Tibet; and mosquitoes (6/131, 4.58%) from Yunnan. A total of 13 JEV envelope (E) gene sequences were amplified in pigs from Heilongjiang (5/13), Jilin (2/13) and Guangxi (6/13). Swine had the highest JEV infection rate of any animal species, and the highest infection rates were found in Heilongjiang. Phylogenetic analysis indicated that the predominant strain in Northern China was genotype I. Mutations were found at residues 76, 95, 123, 138, 244, 474 and 475 of E protein but all sequences had predicted glycosylation sites at ′N154. Three strains lacked the threonine 76 phosphorylation site from non-specific (unsp) and protein kinase G (PKG) site predictions; one lacked the threonine 186 phosphorylation site from protein kinase II (CKII) prediction; and one lacked the tyrosine 90 phosphorylation site from epidermal growth factor receptor (EGFR) prediction. The aim of the current study was to contribute to JEV prevention and control through the characterization of its molecular epidemiology and prediction of functional changes due to E-protein mutations.

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Section: Methodsmentioning

confidence: 99%

Molecular Detection and Genetic Characterization of Japanese Encephalitis Virus in Animals from 11 Provinces in China

Zhao

Gao

Shi

et al. 2023

Viruses

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“…The whole genome of CAdV-1 and CAdV-2 have been uploaded to the GeneBank ( Shibata et al., 1989 ; Morrison et al., 1997 ; Zhu et al., 2021 ). The genetic evolution tree of the virus is shown in Figure 1 .…”

Section: Virus Genomementioning

confidence: 99%

Difference Analysis Between Canine Adenovirus Types 1 And 2

Zhu

Lian

et al. 2022

Front. Cell. Infect. Microbiol.

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Canine adenoviruses (CAdVs) include type 1 (CAdV-1, virulent strain) and type 2 (CAdV-2, attenuated strain). In recent years, the incidences of CAdV infections are increasing. However, they are difficult to distinguish when the symptoms are untypical. It is pivotal to find the differences between the two virus types for scientific, epidemiological, and specific treatment. CAdV-1 (virulent strain) and CAdV-2 (attenuated strain) induced canine hepatitis (ICH) and tracheobronchitis (ITB), respectively, but the clinical symptom is not obvious. CAdV-1 and CAdV-2 have the same genome structure, diameter, morphological features, and cytopathic features, but the same character hinder the diagnose time of the serotypes. CAdV-1 and CAdV-2 have a difference in the genome sequence, coding proteins, viral activity, hemagglutination patterns. After infection, pathogenicity and transmission route are different between the two serotypes. Sequence alignment, PCR, Real time-PCR assay are useful methods to distinguish the two serotypes. The attenuated live CAdV-2 vaccine is currently used to protect against CAdV-1, but it also has a risk. The further research should focus on the pathogenicity mechanism and the useful vaccine for the two serotypes of canine adenovirus.

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“…Various vaccines against infectious hepatitis of the CAV-1 genotype in dogs are used for immunization of puppies [15]. In the manufacture of these vaccines, the virus-containing suspension is assayed for infectivity titer to evaluate its activity in cells.…”

Section: Introductionmentioning

confidence: 99%

Quantitative PCR to determine the titer of infectious activity of the canine hepatitis virus

Doronin,

Mikhalishin,

Shishkova

et al. 2024

E3S Web Conf.

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This article presents data on the development and validation of a method for the indirect determination of the titer of infectivity of canine infectious hepatitis virus of genotype CAV-1 in raw materials for culture vaccines by real-time polymerase chain reaction using the Cq quantification cycle, including the following steps: eluting DNA of canine infectious hepatitis virus genotype CAV-1; performing amplification of a specific fragment orf 16 of canine infectious hepatitis virus genotype CAV-1 DNA using the original specific forward and reverse primers, as well as a molecular probe labeled with fluorescent dye FAM and luminescence quencher RTQ-1: CAV-1-T-F-primer with 5′-CGTAATGGGGAAACCTAGGGG-3′ design, CAV-1-T-R-primer with 5′-TCTGTGTTGTTTCTGTCTTGG-3′ design, and CAV-1-T-Pb-probe with 5′-FAM- CCAATCATCATCTCAACTCAACTAAATGCCGTG-RTQ1-3′ design; calculation of Cq quantification cycle from real-time PCR data; determination of the titer of infectivity of canine infectious hepatitis virus of genotype CAV-1 using a logarithmic function expressed as the equation lg TCAV-1 = -0.2979 × Ct + 9.2595 with an approximation reliability of 0.9941 and amplification efficiency of 99.38%. The analysis time is reduced to 3 h, and the analytical sensitivity is at least 1.0 lg TCD50/cm3.

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The biological characteristics of the canine adenovirus type 1 from fox and the transcriptome analysis of the infected MDCK cell

Cited by 6 publications

References 34 publications

Molecular Detection and Genetic Characterization of Japanese Encephalitis Virus in Animals from 11 Provinces in China

Molecular Detection and Genetic Characterization of Japanese Encephalitis Virus in Animals from 11 Provinces in China

Difference Analysis Between Canine Adenovirus Types 1 And 2

Quantitative PCR to determine the titer of infectious activity of the canine hepatitis virus

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