Difference Analysis Between Canine Adenovirus Types 1 And 2

Zhu, Yanzhu; Xu, Jinfeng; Lian, Shizhen; Zhang, Rui; Hou, Jinyu; Wang, Minchun; Yan, Xijun

doi:10.3389/fcimb.2022.854876

Cited by 8 publications

(6 citation statements)

References 68 publications

(112 reference statements)

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“…JEV nucleic acid sequences were retrieved from the National Center for Biotechnology Information (NCBI, (accessed on 15 December 2022)) [ 14 ] and 31 JEV genome sequences with five genotypes [ 15 ] from GenBank. Genome sequences included 10 genotype I samples: JN381830, JF706268, JN381831, JF706274, HM366552, JF706286, JF706281, JF706270, HQ652538 and DQ404128; 3 genotype II samples: U70406, U70421 and AF217620; 8 genotype III samples: KP164498, KF297915, JN381867, JN381856, JN381857, JF706276, JF706273 and JN381859; 6 genotype IV samples: U70407, U70409, LC461961, JQ429309, AY184212 and U70408 and 4 genotype V samples: JF915894, MF526900, HM596272 and KM677246.…”

Section: Methodsmentioning

confidence: 99%

Molecular Detection and Genetic Characterization of Japanese Encephalitis Virus in Animals from 11 Provinces in China

Zhao

Gao

Shi

et al. 2023

Viruses

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Japanese encephalitis virus (JEV), which uses a mosquito primary vector and swine as a reservoir host, poses a significant risk to human and animal health. JEV can be detected in cattle, goats and dogs. A molecular epidemiological survey of JEV was conducted in 3105 mammals from five species, swine, fox, racoon dog, yak and goat, and 17,300 mosquitoes from 11 Chinese provinces. JEV was detected in pigs from Heilongjiang (12/328, 3.66%), Jilin (17/642, 2.65%), Shandong (14/832, 1.68%), Guangxi (8/278, 2.88%) and Inner Mongolia (9/952, 0.94%); in goats (1/51, 1.96%) from Tibet; and mosquitoes (6/131, 4.58%) from Yunnan. A total of 13 JEV envelope (E) gene sequences were amplified in pigs from Heilongjiang (5/13), Jilin (2/13) and Guangxi (6/13). Swine had the highest JEV infection rate of any animal species, and the highest infection rates were found in Heilongjiang. Phylogenetic analysis indicated that the predominant strain in Northern China was genotype I. Mutations were found at residues 76, 95, 123, 138, 244, 474 and 475 of E protein but all sequences had predicted glycosylation sites at ′N154. Three strains lacked the threonine 76 phosphorylation site from non-specific (unsp) and protein kinase G (PKG) site predictions; one lacked the threonine 186 phosphorylation site from protein kinase II (CKII) prediction; and one lacked the tyrosine 90 phosphorylation site from epidermal growth factor receptor (EGFR) prediction. The aim of the current study was to contribute to JEV prevention and control through the characterization of its molecular epidemiology and prediction of functional changes due to E-protein mutations.

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Section: Methodsmentioning

confidence: 99%

Molecular Detection and Genetic Characterization of Japanese Encephalitis Virus in Animals from 11 Provinces in China

Zhao

Gao

Shi

et al. 2023

Viruses

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“…There is also a core protein bound to virion DNA. The virion nucleic acid is represented by a double-helical linear DNA molecule of about 30,500 bp, which encodes orf 1-orf 30 proteins [5,6]. In the genome of canine infectious hepatitis virus of genotype CAV-1, various conserved regions are identified, in particular, the gene responsible for the synthesis of orf 16 protein (positions in the genome 16757...19474 bp).…”

Section: Introductionmentioning

confidence: 99%

Quantitative PCR to determine the titer of infectious activity of the canine hepatitis virus

Doronin,

Mikhalishin,

Shishkova

et al. 2024

E3S Web Conf.

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This article presents data on the development and validation of a method for the indirect determination of the titer of infectivity of canine infectious hepatitis virus of genotype CAV-1 in raw materials for culture vaccines by real-time polymerase chain reaction using the Cq quantification cycle, including the following steps: eluting DNA of canine infectious hepatitis virus genotype CAV-1; performing amplification of a specific fragment orf 16 of canine infectious hepatitis virus genotype CAV-1 DNA using the original specific forward and reverse primers, as well as a molecular probe labeled with fluorescent dye FAM and luminescence quencher RTQ-1: CAV-1-T-F-primer with 5′-CGTAATGGGGAAACCTAGGGG-3′ design, CAV-1-T-R-primer with 5′-TCTGTGTTGTTTCTGTCTTGG-3′ design, and CAV-1-T-Pb-probe with 5′-FAM- CCAATCATCATCTCAACTCAACTAAATGCCGTG-RTQ1-3′ design; calculation of Cq quantification cycle from real-time PCR data; determination of the titer of infectivity of canine infectious hepatitis virus of genotype CAV-1 using a logarithmic function expressed as the equation lg TCAV-1 = -0.2979 × Ct + 9.2595 with an approximation reliability of 0.9941 and amplification efficiency of 99.38%. The analysis time is reduced to 3 h, and the analytical sensitivity is at least 1.0 lg TCD50/cm3.

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“…The MLV inoculum may contain adventitious infectious agents that allow PRRSV to circulate throughout the herd and spread to other herds ( Mengeling, 2005a ; Mengeling, 2005b ). Moreover, a risk of viral shedding after immunization with live attenuated vaccines has been found, leading to latent infection in healthy animals ( Zhu et al., 2022 ). High safety is the characteristic of inactivated vaccine.…”

Section: Introductionmentioning

confidence: 99%

Immunological evaluation of recombination PRRSV GP3 and GP5 DNA vaccines in vivo

Zhao

Zhang²,

Sun³

et al. 2022

Front. Cell. Infect. Microbiol.

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The porcine reproductive and respiratory syndrome virus (PRRSV) is a threat to the health of pigs worldwide, but commercially available vaccines offer limited protection against PRRSV infection. It is necessary to develop a more effective DNA vaccine. The immunological effects of DNA vaccines with three adjuvants were examined in pigs (Susscrofa domestica) challenged with PRRSV. These DNA vaccines, which encoded PRRSV GP3 and GP5, were formulated with A1, A2, and A3. Serum specific and neutralizing antibodies, IL-4, IFN-γ, IL-2, IL-10, CD4+ and CD8+T-lymphocytes, health status, histopathology, and viral loads were determined. The results showed that the use of adjuvant A3 led to higher levels of neutralizing antibodies and a lower viral load in pigs compared to the other adjuvants. The neutralizing antibody titers of the pVAX-GP35+A1 and pVAX-GP35+A3 groups reached a peak of 1:19 at 35 dpi. The maximum concentration of IL-4 was 136.77 pg/mL in the pVAX-GP35+A3 group. At 35 dpi, the IFN-γ concentration in the pVAX-GP35+A1 group was 227.4 pg/mL. pVAX-GP35+A3 group shows the highest IL-2 and IL-10 expression to the peak of 597.6 pg/mL and 189.1 pg/mL, respectively. We found a formulation demonstrated beneficial immune outcomes. This study provides an alternative vaccine to protect pigs from PRRSV.

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Difference Analysis Between Canine Adenovirus Types 1 And 2

Cited by 8 publications

References 68 publications

Molecular Detection and Genetic Characterization of Japanese Encephalitis Virus in Animals from 11 Provinces in China

Molecular Detection and Genetic Characterization of Japanese Encephalitis Virus in Animals from 11 Provinces in China

Quantitative PCR to determine the titer of infectious activity of the canine hepatitis virus

Immunological evaluation of recombination PRRSV GP3 and GP5 DNA vaccines in vivo

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