The bioleaching potential of a bacterial consortium

Latorre, Mauricio; Cortés, María Paz; Travisany, Dante; Génova, Alex Di; Budinich, Marko; Reyes-Jara, Angélica; Hödar, Christian; González, Maurício; Parada, Pilar; Bobadilla-Fazzini, Roberto A.; Cambiazo, Verónica; Maass, Alejandro

doi:10.1016/j.biortech.2016.07.012

Cited by 65 publications

(40 citation statements)

References 41 publications

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“…ferrooxidans (Hödar et al, 2012). Finally, the third class corresponded to local regulators, highlighting the proteins Fur and CueR (also a MerR family member), controllers of metal homeostasis and oxidative stress damage, two main cellular processes considering the mining environment where Licanantay was isolated (Latorre et al, 2016), during oxidative dissolution, autotrophic organisms are able to use ferrous iron and reduced sulfur compounds as electron donors. In addition, the model contained a total of 10 regulators not controlled by other transcription factors (isolated).…”

Section: A Thiooxidans Licanantay Affinity Transcriptional Regulatormentioning

confidence: 99%

“…In previous work, a number of metabolic processes were linked to the bioleaching capacity of a bacterial consortium that has A. thiooxidans Licanantay as one of its members (Latorre et al, 2016). These processes included known key bioleaching steps, such as iron and RISC oxidation as well as related metabolic features such as sulfur assimilation, biosynthesis of essential components and precursors, electron transfer and energy generation, and biofilm formation.…”

Section: A Thiooxidans Licanantay Metabolic Networkmentioning

confidence: 99%

“…In addition, a set of environmental resistance elements and metabolic pathways presumed relevant to its performance in bioleaching processes have been assigned to this bacterium, most of them related to the oxidation of RISC, metal resistance, biofilm formation, and energy production (Latorre et al, 2016). These results position A. thiooxidans Licanantay as an excellent model to study genomic and metabolic features in terms of gene regulation and metabolic pathways related to the adaptation of this bacterium to the environment of a copper mine.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, A. thiooxidans Licanantay was presented as one of the most relevant participants of a consortium of five natural copper-bioleaching acidophilic bacteria (Latorre et al, 2016). This bacterium was isolated directly from a copper mine in the north of Chile.…”

Section: Introductionmentioning

confidence: 99%

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Integration of Biological Networks for Acidithiobacillus thiooxidans Describes a Modular Gene Regulatory Organization of Bioleaching Pathways

Cortés

Acuña

Travisany

et al. 2020

Front. Mol. Biosci.

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Acidithiobacillus thiooxidans is one of the most studied biomining species, highlighting its ability to oxidize reduced inorganic sulfur compounds, coupled with its elevated capacity to live under an elevated concentration of heavy metals. In this work, using an in silico semi-automatic genome scale approach, two biological networks for A. thiooxidans Licanantay were generated: (i) An affinity transcriptional regulatory network composed of 42 regulatory family genes and 1,501 operons (57% genome coverage) linked through 2,646 putative DNA binding sites (arcs), (ii) A metabolic network reconstruction made of 523 genes and 1,203 reactions (22 pathways related to biomining processes). Through the identification of confident connections between both networks (V-shapes), it was possible to identify a sub-network of transcriptional factor (34 regulators) regulating genes (61 operons) encoding for proteins involved in biomining-related pathways. Network analysis suggested that transcriptional regulation of biomining genes is organized into different modules. The topological parameters showed a high hierarchical organization by levels inside this network (14 layers), highlighting transcription factors CysB, LysR, and IHF as complex modules with high degree and number of controlled pathways. In addition, it was possible to identify transcription factor modules named primary regulators (not controlled by other regulators in the sub-network). Inside this group, CysB was the main module involved in gene regulation of several bioleaching processes. In particular, metabolic processes related to energy metabolism (such as sulfur metabolism) showed a complex integrated regulation, where different primary regulators controlled several genes. In contrast, pathways involved in iron homeostasis and oxidative stress damage are mainly regulated by unique primary regulators, conferring Licanantay an efficient, and specific metal resistance response. This work shows new evidence in terms of transcriptional regulation at a systems level and broadens the study of bioleaching in A. thiooxidans species.

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Section: A Thiooxidans Licanantay Affinity Transcriptional Regulatormentioning

confidence: 99%

Section: A Thiooxidans Licanantay Metabolic Networkmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Integration of Biological Networks for Acidithiobacillus thiooxidans Describes a Modular Gene Regulatory Organization of Bioleaching Pathways

Cortés

Acuña

Travisany

et al. 2020

Front. Mol. Biosci.

Self Cite

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“…Also noteworthy is the effect of microorganisms to mineral bioleaching. The microbiology of leaching environments-including physiology of the most common community members, microbial successions [7,58], the relationship of the population dynamics with environmental factors [59,60], and the influence of community composition on ecosystem functioning [61,62]-have always been the target for research on bioleaching processes and mechanisms. Next generation sequencing technology enables the comprehensive analysis of genomes, which can recover information about their general characteristics, especially their metabolic potential [3,63].…”

Section: Ngs For Genome Analysismentioning

confidence: 99%

Assessment of Bioleaching Microbial Community Structure and Function Based on Next-Generation Sequencing Technologies

et al. 2018

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It is widely known that bioleaching microorganisms have to cope with the complex extreme environment in which microbial ecology relating to community structure and function varies across environmental types. However, analyses of microbial ecology of bioleaching bacteria is still a challenge. To address this challenge, numerous technologies have been developed. In recent years, high-throughput sequencing technologies enabling comprehensive sequencing analysis of cellular RNA and DNA within the reach of most laboratories have been added to the toolbox of microbial ecology. The next-generation sequencing technology allowing processing DNA sequences can produce available draft genomic sequences of more bioleaching bacteria, which provides the opportunity to predict models of genetic and metabolic potential of bioleaching bacteria and ultimately deepens our understanding of bioleaching microorganism. High-throughput sequencing that focuses on targeted phylogenetic marker 16S rRNA has been effectively applied to characterize the community diversity in an ore leaching environment. RNA-seq, another application of high-throughput sequencing to profile RNA, can be for both mapping and quantifying transcriptome and has demonstrated a high efficiency in quantifying the changing expression level of each transcript under different conditions. It has been demonstrated as a powerful tool for dissecting the relationship between genotype and phenotype, leading to interpreting functional elements of the genome and revealing molecular mechanisms of adaption. This review aims to describe the high-throughput sequencing approach for bioleaching environmental microorganisms, particularly focusing on its application associated with challenges.

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Microbial Consortium: A Promising Strategy for Bioleaching of Metals from Industrial Wastes

Chakankar

Hocheng

2022

Advances in Environmental Microbiology

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The bioleaching potential of a bacterial consortium

Cited by 65 publications

References 41 publications

Integration of Biological Networks for Acidithiobacillus thiooxidans Describes a Modular Gene Regulatory Organization of Bioleaching Pathways

Integration of Biological Networks for Acidithiobacillus thiooxidans Describes a Modular Gene Regulatory Organization of Bioleaching Pathways

Assessment of Bioleaching Microbial Community Structure and Function Based on Next-Generation Sequencing Technologies

Microbial Consortium: A Promising Strategy for Bioleaching of Metals from Industrial Wastes

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