The biofilm-positive Staphylococcus epidermidis isolates in raw materials, foodstuffs and on contact surfaces in processing plants

Schlegelová, J.; Babák, Vladimír; Holasová, M.; Dendis, Miloš

doi:10.1007/s12223-008-0078-y

Cited by 14 publications

(9 citation statements)

References 33 publications

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“…One hundred microlitres of the analytical sample were then selectively cultivated on both Baird-Parker and Kranep agars (Merck, Germany) at 37 xC for 48 h. Samples from humans ( Confirmation of S. epidermidis and detection of the ica operon SE705-1 and SE705-2 primers [12] and icaAB-F and icaAB-R primers [13], respectively, were used to amplify a species-specific SE705 sequence and a part of the ica operon, an essential factor involved in biofilm formation. The sequence of 16S-rDNA amplified with UNB1 and UNB2 primers [14] was included as an internal positive control and the PCR reaction was performed as described previously [14].…”

Section: Sample Preparation and Isolation Of S Epidermidismentioning

confidence: 99%

Epidemiology and characterization ofStaphylococcus epidermidisisolates from humans, raw bovine milk and a dairy plant

et al. 2009

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Geographically related Staphylococcus epidermidis isolates from human patients (n=30), dairy farms (farmers and individual raw milk from cattle, n=36) and a dairy plant (n=55) were examined for epidemiological relatedness by pulsed-field gel electrophoresis and, using in vitro methods, for the ability to produce biofilm and antimicrobial resistance. Methicillin-resistant isolates (MRSE) were also identified and characterized. Isolates from farmers and dairy cattle were found to be genetically related, while isolates from human patients were highly diverse. Some dairy plant isolates (18.2%) were closely related to those from dairy farms. Biofilm production and resistance to antimicrobial agents were most typical for isolates from human patients, of which 76.7% were MRSE. Methicillin resistance was also widespread in farm-related isolates (61.1%). This study indicates the possible transmission of S. epidermidis between cattle and farmers. Dairy products were not proven to be an important source of either human infections or methicillin-resistant strains.

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Section: Sample Preparation and Isolation Of S Epidermidismentioning

confidence: 99%

Epidemiology and characterization ofStaphylococcus epidermidisisolates from humans, raw bovine milk and a dairy plant

et al. 2009

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“…The attachment of bacteria with subsequent biofilm development on the food product or the product contact surfaces may compromise food quality or cause health hazards . In this context, Staphylococcus aureus and S. epidermidis are able to form thick multilayered biofilms on different surfaces with concern for the food manufacturers .…”

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confidence: 99%

Enhanced activity of carvacrol against biofilm of Staphylococcus aureus and Staphylococcus epidermidis in an acidic environment

Nostro

Cellini

Zimbalatti

et al. 2012

APMIS

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Carvacrol is an antimicrobial monoterpenic phenol which occurs in many plant essential oils. The aim of this study was to investigate its activity at acidic pH on staphylococcal forming and yet established biofilms, with particular focus to improve its effectiveness on Staphylococcus epidermidis biofilm. The results showed that the subinhibitory doses (1/2, 1/4 and 1/8 MIC) of carvacrol determined a higher reduction of S. epidermidis biofilm formation than that observed at neutral pH. A potentiated inhibitory effect was also observed on established biofilm, carvacrol caused either a strong reduction of biomass (>50%) and bacteria attached to polystyrene (>7 log units). The images of scanning electron microscopy and the gas‐chromatographic analysis support these results. The development of acidic formulations containing carvacrol could be an important tool to control the staphylococcal biofilm in the medical and food environment.

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“…Several studies have reported a higher frequency of distribution of the ica genes and slime production in Staphylococcus isolates from milk (Melo et al, 2013;Rumi et al, 2013;Ciftci et al, 2009), meat, and contact surfaces in processing plants (Schlegelová et al, 2008), indicating the virulence potential of the strains evaluated.…”

Section: Discussionmentioning

confidence: 95%

Detection of exopolysaccharide production and biofilm-related genes in Staphylococcus spp. isolated from a poultry processing plant

Ferreira¹,

Tette²,

Mendonça³

et al. 2014

Food Sci. Technol (Campinas)

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Staphylococcus spp. can survive in biofilms for long periods of time, and they can be transferred from one point to another and cause environmental contamination in food processing. The aim of this study was to detect Staphylococcus strains isolated from a poultry processing plant by the presence of adhesion genes and the phenotypic production of exopolysaccharide. In the present study, the production of exopolysaccharide and the presence of adhesion genes in 65 strains of Staphylococcus spp. were evaluated. All strains of Staphylococcus spp. produced exopolysaccharide, as confirmed by formation of black and opaque colonies in Congo Red Agar. The variation of sucrose content was critical for the production of exopolysaccharide in Congo Red Agar since at low sucrose concentrations all strains presented a characteristic result, i.e., there was no exopolysaccharide production. The atl gene was found in all strains, and the icaA and icaD genes were found in 97% of them. The data obtained suggest that Staphylococcus spp. isolated from the poultry processing plant evaluated has a potential for biofilm formation. An efficient control of this microorganism in food processing environment is necessary as they may represent a potential risk to consumers.

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The biofilm-positive Staphylococcus epidermidis isolates in raw materials, foodstuffs and on contact surfaces in processing plants

Cited by 14 publications

References 33 publications

Epidemiology and characterization ofStaphylococcus epidermidisisolates from humans, raw bovine milk and a dairy plant

Epidemiology and characterization ofStaphylococcus epidermidisisolates from humans, raw bovine milk and a dairy plant

Enhanced activity of carvacrol against biofilm of Staphylococcus aureus and Staphylococcus epidermidis in an acidic environment

Detection of exopolysaccharide production and biofilm-related genes in Staphylococcus spp. isolated from a poultry processing plant

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