Epidemiology and characterization of<i>Staphylococcus epidermidis</i>isolates from humans, raw bovine milk and a dairy plant

Jaglič, Z.; Michu, Elleni; Holasová, M.; Vlkova, H.; Babák, Vladimír; Kolář, Milan; Bardoň, Jan; Schlegelová, J.

doi:10.1017/s0950268809991002

Cited by 26 publications

(32 citation statements)

References 41 publications

(68 reference statements)

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“…In addition, methicillin resistance does not seem to be widely spread among S. aureus isolates from bovine milk, which is in accordance with our results as well (Lim et al 2013;Unnerstad et al 2013). This is in contrast to S. epidermidis, in which methicillin resistance was commonly found (Jaglic et al 2010). However, similarly to the study of Overesch et al (2013), we observed a high prevalence (61.5%) of β-lactamase-producing (penicillin-resistant) isolates.…”

Section: Discussionsupporting

confidence: 91%

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Prevalence of mastitis pathogens in milk from clinically healthy cows

Cervinkova¹,

Vlkova²,

Borodacova³

et al. 2013

Vet. Med.

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A total of 669 individual cow milk samples originating from asymptomatic cows from 16 dairy farms were examined for the presence of microorganisms with the potential to cause mastitis. Coagulase-negative staphylococci clearly predominated (53.5% positive samples) followed by streptococci and enterococci (both occurring in 16.1% samples). Among streptococci, so-called mastitis streptococci (S. uberis, S. dysgalactiae and S. agalactiae) prevailed (11.7% positive samples). Enterobacteriaceae were found in 10.0% samples, most of which (6.6% samples) were positive for Escherichia coli. Yeasts (mainly Candida spp.) were found in 8.2% samples. One of the major mastitis pathogens, Staphylococcus aureus subsp. aureus, was isolated from 9.0% of samples. S. aureus isolates were further characterised in terms of their capability to form biofilm, antimicrobial susceptibility and clonality (PFGE). All S. aureus isolates were capable of biofilm formation and were generally susceptible to the majority of tested antibiotics. The exception was ampicillin, resistance to which was observed in 27.7% isolates. Therefore, the relatively frequent occurrence of S. aureus could be attributed to persistent intramammary infections due to biofilm formation rather than low efficacy of particular antibiotics. PFGE analysis revealed clonal spread of certain S. aureus isolates within and between farms indicating that certain lineages of S. aureus mastitis strains are particularly successful.

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Section: Discussionsupporting

confidence: 91%

“…S. aureus isolates were subjected to PFGE analysis as previously described (Jaglic et al 2010). The isolates were limited to one per sample except for five pairs of morphologically distinct isolates originating from five different samples.…”

Section: Methodsmentioning

confidence: 99%

Prevalence of mastitis pathogens in milk from clinically healthy cows

Cervinkova¹,

Vlkova²,

Borodacova³

et al. 2013

Vet. Med.

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“…The fact that raw milk is pasteurised prior to its processing indicates that other factors (e.g. personnel) may also play an important role in the contamination of the dairy plant environment (Jaglic et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Microbial contamination after sanitation of food contact surfaces in dairy and meat processing plants

Schlegelová¹,

Babák²,

Holasová³

et al. 2010

Czech J. Food Sci.

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Schlegelová J., Babák V., Holasová M., Konstantinová L., Necidová L., Šišák F., Vlková H., Roubal P., Jaglic Z. (2010): Microbial contamination after sanitation of food contact surfaces in dairy and meat processing plants. Czech J. Food Sci., 28: 450-461.The occurrence of listeria monocytogenes, Salmonella spp., Bacillus cereus, Staphylococcus spp., enterococcus spp., and escherichia coli in raw food materials, food products, and on food contact surfaces after sanitation was investigated during the period of 2005-2006 in three dairy cattle farms (120 samples), one dairy (124 samples), and two meat processing plants (160 samples). A total of 1409 isolates were identified. The epidemiological characterisation and determination of the virulence factors and antimicrobial resistance were performed on selected isolates. The level of bacterial contamination generally decreased during the production process (the contamination of food products was lower than that of raw material). However, the contamination of food contact surfaces was relatively high even after sanitation. Moreover, specific microbiological profiles were found on the inside equipment surfaces in dairy facilities, where genetically closely related multi-resistant strains persisting in biofilm communities may occur as demonstrated for staphylococci. Although the occurrence of potentially significant pathogens was not high, the microorganisms such as l. monocytogenes, Salmonella spp., and shiga-toxin positive e. coli principally contaminated the meat processing plants. B. cereus isolates, among which 76% were positive for diarrhogenic enterotoxin, typically occurred on the inside equipment surfaces and in the heat-treated products.

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“…Using PCR, the isolates were also examined for the presence of biofilm genes (icaAB and bap) as described previously (Cervinkova et al 2013). To confirm the persistence and clonality of S. aureus in the herd during the one year period, the isolates were subjected to pulsed field gel electrophoresis (PFGE) analysis as previously described (Jaglic et al 2010).…”

Section: Antimicrobial Susceptibility Biofilm Formation and Moleculamentioning

confidence: 99%

Epidemiology of intramammary infections with Staphylococcus aureus and mastitis streptococci in a dairy cattle herd with a history of recurrent clinical mastitis

Vlkova¹,

Babák²,

Vrtková³

et al. 2017

Polish Journal of Veterinary Sciences

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The aim of the present work was to examine a dairy herd with an anamnesis of recurrent clinical mastitis and decreased milk production. A total of 239 individual cow milk samples originating from asymptomatic cows were collected at four-month intervals and examined mainly for the presence of Staphylococcus aureus and mastitis streptococci using standard cultivation methods. In total, 29.7% and 9.2% samples were positive for S. aureus and mastitis streptococci, respectively. Unlike for mastitis streptococci, the prevalence of animals positive for S. aureus had an increasing trend (p<0.05; Chi-squared test for trend) with rising parity. Despite in vitro susceptibility of S. aureus to potentiated penicillins and cephalosporins, the persistence of S. aureus was observed in cows undergoing intramammary treatment with amoxicillin/clavulanic acid (a potentiated penicillin antibiotic). All isolates of S. aureus were biofilm-positive and had the same macrorestriction pattern. Furthermore, no dependence was observed between the occurrence of S. aureus in milk and previous cases of clinical mastitis, reproductive and periparturient disorders and administration of antibiotics. In contrast to S. aureus, the occurrence of mastitis streptococci in milk was linked with previous cases of clinical mastitis and intramammary administration of antibiotics.

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Epidemiology and characterization ofStaphylococcus epidermidisisolates from humans, raw bovine milk and a dairy plant

Cited by 26 publications

References 41 publications

Prevalence of mastitis pathogens in milk from clinically healthy cows

Prevalence of mastitis pathogens in milk from clinically healthy cows

Microbial contamination after sanitation of food contact surfaces in dairy and meat processing plants

Epidemiology of intramammary infections with Staphylococcus aureus and mastitis streptococci in a dairy cattle herd with a history of recurrent clinical mastitis

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