The bioconversion of waste products from rapeseed processing into keto acids by Yarrowia lipolytica

Cybulski, Krzysztof; Tomaszewska-Hetman, Ludwika; Rakicka, Magdalena; Łaba, Wojciech; Rymowicz, Waldemar; Rywińska, Anita

doi:10.1016/j.indcrop.2018.04.014

Cited by 15 publications

(11 citation statements)

References 33 publications

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“…With the aid of Y. lipolytica strains, biodiesel waste can be converted into microbial protein [26], lipids [27][28][29][30], polyols [31,32], ketoacids [33,34], as well as citric acid (CA) and its salts [35][36][37][38]. In addition to CA, the wild strains Y. lipolytica cultivated on biodiesel waste accumulates ICA in sufficient amounts [39,40].…”

Section: Introductionmentioning

confidence: 99%

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

et al. 2020

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Within this work, the microbial synthesis of (2R,3S)-isocitric acid (ICA), a metabolite of the nonconventional yeast Yarrowia lipolytica, from biodiesel waste, has been studied. The selected strain Y. lipolytica VKM Y-2373 synthesized ICA with citric acid (CA) as a byproduct. This process can be regulated by changing cultivation conditions. The maximal production of ICA with the minimal formation of the byproduct was provided by the use of a concentration of (NH4)2SO4 (6 g/L); the addition of biodiesel waste to cultivation medium in 20–60 g/L portions; maintaining the pH of the cultivation medium at 6, and degree of aeration between 25% and 60% of saturation. Itaconic acid at a concentration of 15 mM favorably influenced the production of ICA by the selected strain. The optimization of cultivation conditions allowed us to increase the concentration of ICA in the culture liquid from 58.32 to 90.2 g/L, the product yield (Y) by 40%, and the ICA/CA ratio from 1.1:1 to 3:1. Research on laboratory animals indicated that ICA counteracted the negative effect of ammonium molybdate (10−5 М) and lead diacetate (10−7 М) on the learning and spatial memory of rats, including those exposed to emotional stress.

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Section: Introductionmentioning

confidence: 99%

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

et al. 2020

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“…All the bioreactor cultures were performed in three replicates and standard deviations were calculated. In the samples, the analysis of the biomass, concentrations of glycerol, pyruvic acid (PA), and α-ketoglutaric acid (KGA) were determined as described by Cybulski et al 2018.…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, after crude substrate application, the changes in the produced ratio of PA:KGA was observed. It should be noted that the modification of the ratio of PA:KGA production might change in the presence of different factors, biotin included, contained as the impurities in crude glycerol (Cybulski et al 2018). It was reported that, in fed-batch culture of Y. lipolytica 374/4 with pure glycerol media after 78 h of cultivation, 61.3 g L −1 of PA was detected, which corresponded to a specific production rate of 0.076 g g −1 h −1 (Morgunov et al 2004).…”

Section: Production Of Pa Under Fed-batch Cultivationsmentioning

confidence: 99%

Yarrowia lipolytica application as a prospective approach for biosynthesis of pyruvic acid from glycerol

et al. 2018

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With the problems related to chemical methods of pyruvic acid (PA) synthesis, a fast-growing interest has been observed in research aiming at reducing the production cost of PA by applying biotechnological methods. This study aimed to investigate the potential applicability of Yarrowia lipolytica Wratislavia 1.31 yeast strain for valorisation of pure and crude glycerol through the production of industrially desired PA. Conditions required for the effective PA biosynthesis, i.e., pH value, thiamine concentration, agitation, and substrate concentration, were examined in batch and fed-batch cultivation modes. The efficient production of PA occurred under the limitation of thiamine (1 µg L −1) and was stimulated by moderate pH (4.5) and agitation (800 rev min −1) of the culture. Under optimal conditions, Y. lipolytica Wratislavia 1.31 was able to produce 85.2 g L −1 of PA with volumetric productivity of 0.90 g L −1 h −1. The yield of PA biosynthesis reached a high level of 1.03 g g −1. Obtained results confirmed the aptitude of Y. lipolytica yeast to produce high amounts of PA from simple glycerol-containing media. Presented process was very promising and might be considered as an attractive alternative for currently used chemical methods of PA synthesis. Keywords Pyruvic acid production • α-ketoglutaric acid • Glycerol • Yarrowia lipolytica • Culture conditions Abbreviations X Biomass (g L −1) PA Pyruvic acid (g L −1) KGA α-ketoglutaric acid (g L −1) Y Pyruvic acid production yield (g g −1) Q Volumetric productivity of pyruvate (g L −1 h −1) q Specific production rate of pyruvic acid (g g −1 h −1) η PA Yield coefficient of PA production

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“…Most of these studies (e.g., seven) use hydrophobic/oily waste streams [47][48][49][50][51][52][53][54], six refer to crude glycerol [48,51,53,[55][56][57], four to agro-industrial and food waste hydrolysates [58][59][60][61], and three to various agro-industrial waste streams including: mango wastes [62], sugarcane molasses [63], and papaya [54]. Various wild and engineered yeast strains were tested for the production of many different products including: SCP [47,50,53,56], biolipids [47][48][49][50][51][52]59,61], organic acids (mainly citric and succinic) [47,55,56,58,60], and enzymes (mainly lipases) [48,53,54,62]. Although most of the studies are focused on other high-value products apart from SCP, the yeast biomass, after recovery of the main product of interest (e.g., biolipids), would still contain high protein content, rendering it useful as supplement in animal feed.…”

Section: Yarrowia Lipolytica Cultivation On Low-cost Awcbsmentioning

confidence: 99%

“…Therefore, Yarrowia lipolytica SCP was considered suitable for use as animal feed protein supplement. Krzysztof et al [56] cultivated Yarrowia lipolytica under various carbon substrates including AWCB like glycerol and fatty acids from biodiesel production. The cultures were grown in a continuous stirred bioreactor, of 2 L operating volume, under aerobic conditions and acidic pH (3.5-4.5).…”

Section: Yarrowia Lipolytica Cultivation On Low-cost Awcbsmentioning

confidence: 99%

Sustainable Animal Feed Protein through the Cultivation of YARROWIA Lipolytica on Agro-Industrial Wastes and by-Products

Patsios

Dedousi²,

Sossidou³

et al. 2020

Sustainability

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Proteins are essential constituents of animal feeds, which comprise mainly vegetable protein (e.g., soybean meal), which is produced and transported globally. The decoupling of protein-production and livestock-growth areas results in protein deficiencies in certain parts of the world, and in significant environmental stress. Alternative, more sustainable protein feeds are necessary to meet the increasing needs, and to decrease the environmental footprint of animal products. Yeast Single Cell Proteins (SCP), produced locally using various agro-industrial by-product streams, have significant potential as alternative animal feed protein. Particularly, Yarrowia lipolytica, an oleaginous, non-pathogenic microorganism has been characterized as a “workhorse” in biotechnological studies, drawing the attention of many researchers. The present review summarizes available resources on critical issues concerning the applicability and commercialization of Yarrowia lipolytica as an environment-friendly protein source for animal feed. It discusses the sustainability of the yeast SCP production process, it presents the recent advances concerning Yarrowia lipolytica cultivation on low-cost agro-industrial by-products, and it stresses the effects on the health and welfare of productive animals due to the inclusion of Yarrowia lipolytica in their diet. The data presented in this study should facilitate relative research advancement and the commercialization of Yarrowia lipolytica’s use as an alternative protein source/supplement for animal feeds.

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The bioconversion of waste products from rapeseed processing into keto acids by Yarrowia lipolytica

Cited by 15 publications

References 33 publications

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

Microbiological Production of Isocitric Acid from Biodiesel Waste and Its Effect on Spatial Memory

Yarrowia lipolytica application as a prospective approach for biosynthesis of pyruvic acid from glycerol

Sustainable Animal Feed Protein through the Cultivation of YARROWIA Lipolytica on Agro-Industrial Wastes and by-Products

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