The biocontrol agent Streptomyces pactum increases Pseudomonas koreensis populations in the rhizosphere by enhancing chemotaxis and biofilm formation

Qiao, Guo; Shi, Meihong; Chen, Lin; Zhou, Jinhua; Zhang, Lixin; Li, Yulong; Xue, Qiao; Lai, Hung-Yu

doi:10.1016/j.soilbio.2020.107755

Cited by 47 publications

(23 citation statements)

References 61 publications

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“…( Raaijmakers and Weller, 1998 ; Mendes et al, 2011 ; Hu et al, 2016 ), Bacillus spp. ( Wei et al, 2011 ; Guo et al, 2020 ), Burkholderia spp. ( Nion and Toyota, 2008 ), and Streptomyces spp.…”

Section: Introductionmentioning

confidence: 99%

Exploring Biocontrol Agents From Microbial Keystone Taxa Associated to Suppressive Soil: A New Attempt for a Biocontrol Strategy

et al. 2021

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Recent studies have observed differing microbiomes between disease-suppressive and disease-conducive soils. However, it remains unclear whether the microbial keystone taxa in suppressive soil are critical for the suppression of diseases. Bacterial wilt is a common soil-borne disease caused by Ralstonia solanacearum that affects tobacco plants. In this study, two contrasting tobacco fields with bacterial wilt disease incidences of 0% (disease suppressive) and 100% (disease conducive) were observed. Through amplicon sequencing, as expected, a high abundance of Ralstonia was found in the disease-conducive soil, while large amounts of potential beneficial bacteria were found in the disease-suppressive soil. In the fungal community, an abundance of the Fusarium genus, which contains species that cause Fusarium wilt, showed a positive correlation (p < 0.001) with the abundance of Ralstonia. Network analysis revealed that the healthy plants had more complex bacterial networks than the diseased plants. A total of 9 and 13 bacterial keystone taxa were identified from the disease-suppressive soil and healthy root, respectively. Accumulated abundance of these bacterial keystones showed a negative correlation (p < 0.001) with the abundance of Ralstonia. To complement network analysis, culturable strains were isolated, and three species belonging to Pseudomonas showed high 16S rRNA gene similarity (98.4–100%) with keystone taxa. These strains displayed strong inhibition on pathogens and reduced the incidence of bacterial wilt disease in greenhouse condition. This study highlighted the importance of keystone species in the protection of crops against pathogen infection and proposed an approach to obtain beneficial bacteria through identifying keystone species, avoiding large-scale bacterial isolation and cultivation.

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Section: Introductionmentioning

confidence: 99%

Exploring Biocontrol Agents From Microbial Keystone Taxa Associated to Suppressive Soil: A New Attempt for a Biocontrol Strategy

et al. 2021

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“…The ability to move in a controlled manner confers distinct advantages to rhizospheric bacteria, particularly during colonization. Flagellar development plays a vital role in the motility of bacteria especially during times of environmental stress, and it has been established that nonmotile bacteria are severely impaired in colonizing and surviving within the rhizosphere ( Lugtenberg et al, 2001 ; Capdevila et al, 2004 ; Guo et al, 2020 ). However, the bacteria flagellar motor is the most complex structure of the bacterial cell, requiring over 50 genes across multiple operons, of which the MBSA-MJ1 genome contains the entire suite of genes ( Chilcott and Hughes, 2000 ; Armitage and Berry, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…These findings corroborate the results from the in vitro motility experiment that confirmed MBSA-MJ1’s ability to move spatially in semi-solid agar media. Chemotaxis is another important trait in competitive colonization as it controls the direction of flagellar rotation and movement ( De Weert et al, 2002 ; Danhorn and Fuqua, 2007 ; Guo et al, 2020 ), and the three chemotaxis-related TCS identified in the genome of MBSA-MJ1 likely play a fundamental role in this complex function. PGPR that colonize the rhizosphere have also been known to colonize plant tissue as endophytes ( Santoyo et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Genomic Analysis of Serratia plymuthica MBSA-MJ1: A Plant Growth Promoting Rhizobacteria That Improves Water Stress Tolerance in Greenhouse Ornamentals

Nordstedt

Jones

2021

Front. Microbiol.

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Water stress decreases the health and quality of horticulture crops by inhibiting photosynthesis, transpiration, and nutrient uptake. Application of plant growth promoting rhizobacteria (PGPR) can increase the growth, stress tolerance, and overall quality of field and greenhouse grown crops subjected to water stress. Here, we evaluated Serratia plymuthica MBSA-MJ1 for its ability to increase plant growth and quality of Petunia × hybrida (petunia), Impatiens walleriana (impatiens), and Viola × wittrockiana (pansy) plants recovering from severe water stress. Plants were treated weekly with inoculum of MBSA-MJ1, and plant growth and quality were evaluated 2 weeks after recovery from water stress. Application of S. plymuthica MBSA-MJ1 increased the visual quality and shoot biomass of petunia and impatiens and increased the flower number of petunia after recovery from water stress. In addition, in vitro characterizations showed that MBSA-MJ1 is a motile bacterium with moderate levels of antibiotic resistance that can withstand osmotic stress. Further, comprehensive genomic analyses identified genes putatively involved in bacterial osmotic and oxidative stress responses and the synthesis of osmoprotectants and vitamins that could potentially be involved in increasing plant water stress tolerance. This work provides a better understanding of potential mechanisms involved in beneficial plant-microbe interactions under abiotic stress using a novel S. plymuthica strain as a model.

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“…Moreover, using RNA sequencing, Guo et al (2020) proved that Streptomyces pactum Act12 inoculation enhanced tomato rhizosphere colonization and competition by indigenous P . koreensis GS via the promotion of swimming motility, biofilm formation, and environmental adaptation [ 44 ].…”

Section: Mechanisms Of Pgpr In Disease Suppressionmentioning

confidence: 99%

“…Second, individual PGPR strains/species exert complementary disease-suppressive mechanisms (traits) [ 132 , 135 ], such that when one mechanism is ineffective under a particular set of conditions, the others can compensate for the former absence. Third, the effects of similar or different mechanisms of action employed by different strains may augment quantitatively in a beneficial way [ 44 , 112 ] ( Figure 1 b).…”

Section: Critical Approaches Towards Developing Successful Pgpr Mixturesmentioning

confidence: 99%

Pathogen Biocontrol Using Plant Growth-Promoting Bacteria (PGPR): Role of Bacterial Diversity

et al. 2021

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A vast microbial community inhabits in the rhizosphere, among which, specialized bacteria known as Plant Growth-Promoting Rhizobacteria (PGPR) confer benefits to host plants including growth promotion and disease suppression. PGPR taxa vary in the ways whereby they curtail the negative effects of invading plant pathogens. However, a cumulative or synergistic effect does not always ensue when a bacterial consortium is used. In this review, we reassess the disease-suppressive mechanisms of PGPR and present explanations and illustrations for functional diversity and/or stability among PGPR taxa regarding these mechanisms. We also provide evidence of benefits when PGPR mixtures, rather than individuals, are used for protecting crops from various diseases, and underscore the critical determinant factors for successful use of PGPR mixtures. Then, we evaluate the challenges of and limitations to achieving the desired outcomes from strain/species-rich bacterial assemblages, particularly in relation to their role for plant disease management. In addition, towards locating additive or synergistic outcomes, we highlight why and how the benefits conferred need to be categorized and quantified when different strains/species of PGPR are used in combinations. Finally, we highlight the critical approaches needed for developing PGPR mixtures with improved efficacy and stability as biocontrols for utilization in agricultural fields.

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The biocontrol agent Streptomyces pactum increases Pseudomonas koreensis populations in the rhizosphere by enhancing chemotaxis and biofilm formation

Cited by 47 publications

References 61 publications

Exploring Biocontrol Agents From Microbial Keystone Taxa Associated to Suppressive Soil: A New Attempt for a Biocontrol Strategy

Exploring Biocontrol Agents From Microbial Keystone Taxa Associated to Suppressive Soil: A New Attempt for a Biocontrol Strategy

Genomic Analysis of Serratia plymuthica MBSA-MJ1: A Plant Growth Promoting Rhizobacteria That Improves Water Stress Tolerance in Greenhouse Ornamentals

Pathogen Biocontrol Using Plant Growth-Promoting Bacteria (PGPR): Role of Bacterial Diversity

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