The biocompatibility of titanium in a buffer solution: compared effects of a thin film of TiO2 deposited by MOCVD and of collagen deposited from a gel

Abstract

This study aims at evaluating the biocompatibility of titanium surfaces modified according two different ways: (i) deposition of a bio-inert, thin film of rutile TiO(2) by chemical vapour deposition (MOCVD), and (ii) biochemical treatment with collagen gel, in order to obtain a bio-interactive coating. Behind the comparison is the idea that either the bio-inert or the bio-active coating has specific advantages when applied to implant treatment, such as the low price of the collagen treatment for instance. The stability in buffer solution was evaluated by open circuit potential (OCP) for medium time and cyclic voltametry. The OCP stabilized after 5.10(4) min for all the specimens except the collagen treated sample which presented a stable OCP from the first minutes. MOCVD treated samples stabilized to more electropositive values. Numeric results were statistically analysed to obtain the regression equations for long time predictable evolution. The corrosion parameters determined from cyclic curves revealed that the MOCVD treatment is an efficient way to improve corrosion resistance. Human dermal fibroblasts were selected for cell culture tests, taking into account that these cells are present in all bio-interfaces, being the main cellular type of connective tissue. The cells grew on either type of surface without phenotype modification. From the reduction of yellow, water-soluble 3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT cytotoxicity test), MOCVD treated samples offer better viability than mechanically polished Ti and collagen treated samples as well. Cell spreading, as evaluated from microscope images processed by the program Sigma Scan, showed also enhancement upon surface modification. Depending on the experimental conditions, MOCVD deposited TiO(2) exhibits different nanostructures that may influence biological behaviour. The results demonstrate the capacity of integration in simulated physiologic liquids for an implant pretreated by either method.

Influence of Coating Parameter and Sintering Atmosphere on the Corrosion Resistance Behavior of Electrophoretically Deposited Composite Coatings

“…The obtained results are in good resemblance with previous corrosion study on titanium substarte (Ti-6Al-4V) [26]. For improved resistance to corrosion and biocompatibility, the individual titania coating itself was not sufficient to inhibit the substrate.…”

Effect of nano-topographical features of Ti/TiO2 electrode surface on cell response and electrochemical stability in artificial saliva

“…In the last decades, many approaches have been made to improve the surface activity of Ti-and Ti alloys, by modifying TiO 2 properties using chemical and physical procedures, while taking into account that being a bioinert material, a Ti implant becomes encapsulated into the living body by a fibrous tissue. The changing crystallinity and roughness of TiO 2 as example using MOCVD (physical chemical deposition from organo metallic precursor) [16,17] or various coatings as hydroxyapatite [18][19][20] or collagen [21] is a way to get a better integration into the bone and represented conventional methods in surface modification techniques. [22][23][24][25][26][27].…”

MOCVD-Fabricated TiO2Thin Films: Influence of Growth Conditions on Fibroblast Cells Culture

“…We have been using the LP-MOCVD technique to grow TiO 2 thin films on titanium with varied morphologies and micro-(nano-) structures with the view of studying the influence of film characteristics on cells behaviour in culture systems and on the protection of Ti substrates against corrosion by biological fluids [20,21]. The aim of the present study was to grow various thin films of TiO 2 on titanium substrates using the MOCVD technique and to assess in vitro compatibility of the films by using a human gingival fibroblast cell line (HGF-1; ATCC).…”