The Biocompatibility of Dental Graded Nano-Glass-Zirconia Material After Aging

Abstract

ᅟA graded nano-glass/zirconia (G/Z) system has been developed via the infiltration of nano-glass into a nano-zirconia surface, which is advantageous for robust core-veneer bonds. The aging issue is a key for yttrium-stabilized tetragonal zirconia polycrystals (Y-TZPs), and therefore, it is necessary to evaluate the influence of aging degradation on the biocompatibility of G/Z systems before their possible clinical application. Herein, such biocompatibility testing was performed with human gingival fibroblasts (HGFs) seeded onto unaged/aged G/Z and Y-TZP for 2–72 h. Assessments included an oral mucous membrane irritation test in conjunction with analyses of cell viability, cell adhesion, and oxidative stress responses. Significant metabolic decreases in aged G/Z- and Y-TZP-treated cells were observed at 72 h. G/Z did not elicit any significant differences in cell viability compared with Y-TZP over 72 h both before and after aging. The oxidative stress data for the aged G/Z- and Y-TZP-treated cells showed a significant increase at 72 h. The G/Z specimens did not elicit any significant differences in ROS production compared with Y-TZP over 72 h both before and after aging. The cell adhesion rates of both G/Z and Y-TZP increased significantly after aging. The cell adhesion rates of G/Z and Y-TZP were not significantly different before and after aging. According to the oral mucous membrane irritation test, scores for macroscopic and microscopic observations for both the aged G/Z and unaged G/Z sides were 0, demonstrating no consequent irritation.ConclusionsThe excellent biocompatibility of G/Z indicates that it has potential for future clinical applications.

Biocompatibility of Polymer and Ceramic CAD/CAM Materials with Human Gingival Fibroblasts (HGFs)

“…In accordance with the obtained results, it cannot be said that these materials are totally inert; nevertheless, the cytotoxic values after 72 h are within the biocompatibility range for clinical use, as other authors have claimed [17,72,73].…”

<p>Toxicity, biodistribution and oxidative damage caused by zirconia nanoparticles after intravenous injection</p>

“…ZrO 2 nanoparticles have been applied in dentistry, metal joint, drug carrier and other fields due to their good biocompatibility and strong stability 4–7. Especially in dentistry, dental materials based on ZrO 2 have been the mainstream of high-end products because of the following advantages of these materials including strong corrosion resistance, pure white color and good compatibility 8–11. As metal joint, ZrO 2 has significant advantages as good biocompatibility, strong stability and flexural strength 12.…”

Dual response of fibroblasts viability and Porphyromonas gingivalis adhesion on nanostructured zirconia abutment surfaces

“…confirming that zirconia was not a cytotoxic biomaterial. 38 Despite absence of cytotoxicity effects, CZr and PZr group highly expressed both ECM-related genes and proteins (COL-I, VCL, and FN), which were confirmed by the qRT-PCR assay as well as western blotting analysis. Consistently, COL-I secretion on different surfaces presented the following trend of PZr > CZr > Zr, which further confirmed an increase in roughness could decrease deposition of collagen fibers.…”

“…Interestingly, our CCK‐8 assay results demonstrated the absence of cytotoxic effects on HGFs in direct contact with Zr, CZr and PZr surfaces since the proliferation rate of HGFs was higher as time went by. confirming that zirconia was not a cytotoxic biomaterial 38 …”