The bio-functional role of calcium in mesoporous silica xerogels on the responses of osteoblasts in vitro

Zhou, Hao; Wei, Jie; Wu, Xiaohui; Shi, Jianlin; Liu, Changsheng; Jia, Junfeng; Dai, Chenglong; Gan, Qi

doi:10.1007/s10856-010-4083-8

Cited by 31 publications

(34 citation statements)

References 42 publications

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“…While many in vitro studies have demonstrated the proper ionic doses favorable for cell proliferation and osteogenesis, a limited number of studies have examined the functions in 3D scaffolds. Different Ca concentrations incorporated into a collagen gel showed that low concentrations (2-4 mM) promoted osteoblast proliferation, medium concentrations (6-8 mM) induced cellular differentiation and mineralization, whereas concentrations greater than 8 mM induced cytotoxicity [154]. On the other hand, high Ca content incorporated within silica based microspheres showed enhanced cell proliferation [155], while the increased Ca level was shown to be related with lower levels of ALP activity [151].…”

Section: Control Of Therapeutic Ionsmentioning

confidence: 99%

Therapeutically relevant aspects in bone repair and regeneration

et al. 2015

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Over the past few years, attention has been focused on the therapeutic roles in designing bone scaffolds for successful repair and regeneration. Indeed, biologically dynamic events in the bone healing process involve many of the molecules and cells adherent to the scaffold. Recent bone scaffolds have been designed considering intrinsic chemical and physical factors and exogenous/extrinsic cues that induce bone regeneration. Here, we attempt to topically review the current trends and to suggest featured strategies for the design of therapeutically relevant bone scaffolds taking into account recent studies and applications.

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Section: Control Of Therapeutic Ionsmentioning

confidence: 99%

Therapeutically relevant aspects in bone repair and regeneration

et al. 2015

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“…There have been many reports of enhanced cellular bioactivity using biosilicates (P 2 O 5 -Na 2 O-CaOSiO 2 ), 1 calcium-magnesium-silicate ceramics, 2 calcium-silica ceramics, 3 and bioactive glass (Ca-Sr-Na-Zn-Si). 4 Mesoporous silica particles are also being used for controlled release scaffolds, 5 and many bioceramics include a silica component.…”

Section: Introductionmentioning

confidence: 99%

“…1,2 A mixture of silicon and calcium, 3,11 or mixtures with various other materials, including zinc and magnesium, 12 improve both gene expression and biological performance.…”

Section: Introductionmentioning

confidence: 99%

Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation

Kim¹,

Joe²,

Kim³

et al. 2015

IJN

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Background Silicon dioxide composites have been found to enhance the mechanical properties of scaffolds and to support growth of human adipose tissue-derived stem cells (hADSCs) both in vitro and in vivo. Silica (silicon dioxide alone) exists as differently sized particles when suspended in culture medium, but it is not clear whether particle size influences the beneficial effect of silicon dioxide on hADSCs. In this study, we examined the effect of different sized particles on growth and mitogen-activated protein kinase signaling in hADSCs. Methods Silica gel was prepared by a chemical reaction using hydrochloric acid and sodium silicate, washed, sterilized, and suspended in serum-free culture medium for 48 hours, and then sequentially filtered through a 0.22 μm filter (filtrate containing nanoparticles smaller than 220 nm; silica NPs). hADSCs were incubated with silica NPs or 3 μm silica microparticles (MPs), examined by transmission electron microscopy, and assayed for cell proliferation, apoptosis, and mitogen-activated protein kinase signaling. Results Eighty-nine percent of the silica NPs were around 50–120 nm in size. When hADSCs were treated with the study particles, silica NPs were observed in endocytosed vacuoles in the cytosol of hADSCs, but silica MPs showed no cell entry. Silica NPs increased the proliferation of hADSCs, but silica MPs had no significant effect in this regard. Instead, silica MPs induced slight apoptosis. Silica NPs increased phosphorylation of extracellular signal-related kinase (ERK)1/2, while silica MPs increased phosphorylation of p38. Silica NPs had no effect on phosphorylation of Janus kinase or p38. Pretreatment with PD98059, a MEK inhibitor, prevented the ERK1/2 phosphorylation and proliferation induced by silica NPs. Conclusion Scaffolds containing silicon dioxide for tissue engineering may enhance cell growth through ERK1/2 activation only when NPs around 50–120 nm in size are included, and single component silica-derived NPs could be useful for bioscaffolds in stem cell therapy.

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“…Previous studies have shown that ionic dissolution products containing Ca and Si from materials can stimulate osteoblast proliferation and gene expression [39]. Previously Hench et al showed that approximately 17-21 ppm of soluble Si and 60-88 ppm of soluble Ca ions were required for osteoblasts to grow, differentiate and form a mineralized extracellular matrix and create bone nodules [40].…”

Section: Figurementioning

confidence: 99%

Long term effects of bioactive glass particulates on dental pulp stem cells in vitro

Gholami¹,

Labbaf²,

Houreh³

et al. 2017

Biomedical Glasses

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Bioactive glasses (BG) are known for their ability to induce bone formation by the action of their dissolution products. Glasses can deliver active ions at a sustained rate, determined by their composition and surface area. Nanoporous sol-gel derived BGs can biodegrade rapidly, which can lead to a detrimental burst release of ions and a pH rise. The addition of phosphate into the glass can buffer the pH during dissolution. Here, dissolution of BG with composition 60 mol% SiO 2 , 28 mol% CaO and 12 mol% P 2 O 5 at 600 µg/ml were investigated. Initially, the dissolution and apatite formation of the BG particulates were examined in simulated body fluid using FTIR and XRD. BG particulates were indirectly exposed to dental pulp stem cells, and the effect of 14 days continuous ion release on human dental pulp stem cells (hDPSC) viability and differentiation was evaluated. Alamar blue assay showed that cell proliferation was not inhibited by the continuous release of Ca, P and soluble silica. In fact, hDPSC in the presence of BG particulate displayed a higher density of mineralized nodules than untreated cells, as assessed by Alizarin red. The results will have a great contribution to the in vivo application of this particular BG.

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The bio-functional role of calcium in mesoporous silica xerogels on the responses of osteoblasts in vitro

Cited by 31 publications

References 42 publications

Therapeutically relevant aspects in bone repair and regeneration

Therapeutically relevant aspects in bone repair and regeneration

Silica nanoparticles increase human adipose tissue-derived stem cell proliferation through ERK1/2 activation

Long term effects of bioactive glass particulates on dental pulp stem cells in vitro

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