Therapeutically relevant aspects in bone repair and regeneration

Pérez, Román A.; Seo, Seog Jin; Won, Jong‐Eun; Lee, Eun Jung; Jang, Jingon; Knowles, Jonathan C.; Kim, Hae Won

doi:10.1016/j.mattod.2015.06.011

Cited by 109 publications

(84 citation statements)

References 200 publications

(158 reference statements)

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“…The three-dimensional (3D) porous scaffold creates and maintains a 3D space within the defect in vivo and provide a framework for growth of new tissue that eventually replace the scaffold. The scaffold not only provides a supporting matrix for the recruitment of bone cells, but also provides an essential environment for cells to spread, migrate, multiply and differentiate into specific lineages [39, 40]. …”

Section: Resultsmentioning

confidence: 99%

Regeneration of bone using nanoplex delivery of FGF-2 and BMP-2 genes in diaphyseal long bone radial defects in a diabetic rabbit model

Khorsand

Nicholson

et al. 2017

Journal of Controlled Release

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Bone fracture healing impairment related to systemic diseases such as diabetes can be addressed by growth factor augmentation. We previously reported that growth factors such as fibroblast growth factor-2 (FGF-2) and bone morphogenetic protein-2 (BMP-2) work synergistically to encourage osteogenesis in vitro. In this report, we investigated if BMP-2 and FGF-2 together can synergistically promote bone repair in a leporine model of diabetes mellitus, a condition that is known to be detrimental to union. We utilized two kinds of plasmid DNA encoding either BMP-2 or FGF-2 formulated into polyethylenimine (PEI) complexes. The fabricated nanoplexes were assessed for their size, charge, in vitro cytotoxicity, and capacity to transfect human bone marrow stromal cells (BMSCs). Using diaphyseal long bone radial defects in a diabetic rabbit model it was demonstrated that co-delivery of PEI-(pBMP-2+pFGF-2) embedded in collagen scaffolds resulted in a significant improvement in bone regeneration compared to PEI-pBMP-2 embedded in collagen scaffolds alone. This study demonstrated that scaffolds loaded with PEI-(pBMP-2+pFGF-2) could be an effective way of promoting bone regeneration in patients with diabetes.

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Section: Resultsmentioning

confidence: 99%

Regeneration of bone using nanoplex delivery of FGF-2 and BMP-2 genes in diaphyseal long bone radial defects in a diabetic rabbit model

Khorsand

Nicholson

et al. 2017

Journal of Controlled Release

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“…Furthermore, the hybrid deposits facilitate the attachment of the hMSCs stem cells, again an added favorable property to the development of an advanced barrier membrane [30]. Considering earlier contributions that polyP microparticles promote cell growth/differentiation also under physiological normoxic conditions [31] it was relevant to confirm that this property was also retained under the spun fiber mat conditions. The rubber-like elasticity of the collagen/polyP matrices, developed here, can be attributed to the helical tertiary structure and conformation of collagen [32].…”

Section: Discussionmentioning

confidence: 99%

Morphogenetically-Active Barrier Membrane for Guided Bone Regeneration, Based on Amorphous Polyphosphate

et al. 2017

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We describe a novel regeneratively-active barrier membrane which consists of a durable electrospun poly(ε-caprolactone) (PCL) net covered with a morphogenetically-active biohybrid material composed of collagen and inorganic polyphosphate (polyP). The patch-like fibrous collagen structures are decorated with small amorphous polyP nanoparticles (50 nm) formed by precipitation of this energy-rich and enzyme-degradable (alkaline phosphatase) polymer in the presence of calcium ions. The fabricated PCL-polyP/collagen hybrid mats are characterized by advantageous biomechanical properties, such as enhanced flexibility and stretchability with almost unaltered tensile strength of the PCL net. The polyP/collagen material promotes the attachment and increases the viability/metabolic activity of human mesenchymal stem cells compared to cells grown on non-coated mats. The gene expression studies revealed that cells, growing onto polyP/collagen coated mats show a significantly (two-fold) higher upregulation of the steady-state-expression of the angiopoietin-2 gene used as an early marker for wound healing than cells cultivated onto non-coated mats. Based on our results we propose that amorphous polyP, stabilized onto a collagen matrix, might be a promising component of functionally-active barrier membranes for guided tissue regeneration in medicine and dentistry.

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“…Normally it involves the use of porous threedimensional (3D) scaffolds that along with cells and bioactive factors provide structural support for cells to spread, migrate, multiply and differentiate and new tissue being formed [12,73]. In this way, scaffolds act as a temporary ECM inducing the natural processes of tissue regeneration and development [74].…”

Section: Bone Tissue Engineeringmentioning

confidence: 99%

Synthetic and Marine-Derived Porous Bone Graft Substitutes

Neto¹,

Ferreira²

2018

Preprint

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Bone is a dynamic tissue with the capacity of repair and regeneration in specific conditions. Nevertheless, due to the increased incidence of bone disorders, the need of bone grafts has been growing over the past decades and the development of a bone graft with optimal properties remains a clinical challenge. This review addresses the bone properties (morphology, composition and their repair and regeneration capacity) and then is focused on the potential strategies for bone repair and regeneration. It describes the requirements for designing a suitable scaffold material, types of materials (polymers, ceramics and composites) and techniques to obtain the porous structures (additive manufacturing techniques/robocasting or derived from marine skeletons) for bone tissue engineering applications. The main objective of this review is to gather the knowledge on the materials and methods for the production of scaffolds for bone tissue engineering and highlight that natural materials, namely the marine skeletons represent a promising alternative.

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Therapeutically relevant aspects in bone repair and regeneration

Cited by 109 publications

References 200 publications

Regeneration of bone using nanoplex delivery of FGF-2 and BMP-2 genes in diaphyseal long bone radial defects in a diabetic rabbit model

Regeneration of bone using nanoplex delivery of FGF-2 and BMP-2 genes in diaphyseal long bone radial defects in a diabetic rabbit model

Morphogenetically-Active Barrier Membrane for Guided Bone Regeneration, Based on Amorphous Polyphosphate

Synthetic and Marine-Derived Porous Bone Graft Substitutes

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