The Binding Energy of Two Nitrilotriacetate Groups Sharing a Nickel Ion

Tareste, David; Pincet, Frédéric; Brellier, Marie; Mioskowski, Charles; Perez, Éric

doi:10.1021/ja043525q

Cited by 19 publications

(14 citation statements)

References 41 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Normalizing the adhesion energy by ⌫, which is the cadherin surface density determined by plasmon resonance and radiolabeling, allows comparisons between measurements with different protein preparations. Assuming an equilibrium distribution between bound and free states, the estimated bond energy is E A ϭ ⌫(E b ͞(1 ϩ exp(ϪE b ͞ k B T))) (58). Because stresses may be unevenly distributed in the contact region, the above estimate is a lower bound.…”

Section: Methodsmentioning

confidence: 99%

Similarities between heterophilic and homophilic cadherin adhesion

Prakasam

Maruthamuthu

Leckband³

2006

Proc. Natl. Acad. Sci. U.S.A.

102

107

View full text Add to dashboard Cite

The mechanism that drives the segregation of cells into tissuespecific subpopulations during development is largely attributed to differences in intercellular adhesion. This process requires the cadherin family of calcium-dependent glycoproteins. A widely held view is that protein-level discrimination between different cadherins on cell surfaces drives this sorting process. Despite this postulated molecular selectivity, adhesion selectivity has not been quantitatively verified at the protein level. In this work, molecular force measurements and bead aggregation assays tested whether differences in cadherin bond strengths could account for cell sorting in vivo and in vitro. Studies were conducted with chicken N-cadherin, canine E-cadherin, and Xenopus C-cadherin. Both qualitative bead aggregation and quantitative force measurements show that the cadherins cross-react. Furthermore, heterophilic adhesion is not substantially weaker than homophilic adhesion, and the measured differences in adhesion do not correlate with cell sorting behavior. These results suggest that the basis for cell segregation during morphogenesis does not map exclusively to protein-level differences in cadherin adhesion.cell adhesion ͉ force probe ͉ selectivity ͉ surface forces apparatus B oth the formation of distinct tissues during morphogenesis and the maintenance of adult tissue structure are regulated by adhesive contacts between cells. One family of adhesion proteins, cadherins, is critical to several processes associated with the formation and maintenance of tissues. The genetic regulation of the spatiotemporal expression patterns of cadherins in vivo is believed to play a central role in embryogenesis, cell differentiation, and the maintenance of the multicellular structure of an organism (1-5). One prevalent hypothesis is that differences in adhesion between cells direct cell segregation during morphogenesis, analogous to liquid-liquid phase separation (6). A fundamental unresolved question is whether this sorting out is encoded by protein-specific or cell-specific differences in adhesion. A common hypothesis is that preferential binding between identical cadherins (homophilic) relative to heterophilic binding between dissimilar cadherins induces the cell segregation.Both the adhesive and the selectivity functions of classic cadherins map to the extracellular (EC) region (7), which comprises five tandemly arranged EC domains, EC1-5, numbered from the outermost domain (Fig. 1) (8). Classical cadherins also contain a transmembrane segment and a cytoplasmic domain. Cadherins form multiple bonds that require different EC domains (9-12). A common feature of the classical cadherins is that the N-terminal domains bind by inserting the tryptophan 2 (W2) residue from one protein into a hydrophobic pocket on an opposed N-terminal domain (13-16). However, given the weak adhesion between the outer domains (11, 12), it is questionable whether the tryptophan binding accounts for both cadherin selectivity and robust adhesion.The most extensively ci...

show abstract

Section: Methodsmentioning

confidence: 99%

Similarities between heterophilic and homophilic cadherin adhesion

Prakasam

Maruthamuthu

Leckband³

2006

Proc. Natl. Acad. Sci. U.S.A.

102

107

View full text Add to dashboard Cite

show abstract

“…Both the normal and reverse titration experiments were analyzed by using, respectively, the "two-site" and "deconvoluted two-site" regression models proposed from using the Origin software. [30] The results are shown in Table 1.…”

Section: Resultsmentioning

confidence: 99%

“…Theoretical calculation of association enthalpies related to species 2:1, 1:1, and 2:2 based on published enthalpy values associated with complexes Ni 2 + -NTA and NTA-Ni 2 + -NTA. [30] Entry Complex Reaction equilibrium Association enthalpy Figure 10. Postulated mechanism for the formation of ternary complexes H, I, and J with histidine tags, starting from species B and D.…”

Section: Entrymentioning

confidence: 99%

See 1 more Smart Citation

Insight into the Complexation Mode of Bis(nitrilotriacetic acid) (NTA) Ligands with Ni²⁺ Involved in the Labeling of Histidine‐Tagged Proteins

Brellier

Barlaam

Mioskowski

et al. 2009

Chemistry A European J

Self Cite

View full text Add to dashboard Cite

According to literature reports and our own findings, the binding of new Ni(2+)-preloaded bis(nitrilotriacetic acid) (NTA) ligands with polyhistidine-tagged proteins has been found to be accompanied by a one- to two-order-of-magnitude increase in affinity, compared to the binding of a single Ni(2+)-preloaded NTA moiety. In spite of the introduction of a second NTA chelating group, a cooperative effect that is less than the theoretical maximum has been observed. Herein, we present a rational explanation for the observed stability of the ternary complex involving the postulated bis-NTA-(Ni(2+))(2) species and multivalent polyhistidine tags. We have found that prior to the formation of the ternary complex, the Ni(2+)-preloading step of bis-NTA ligands does not form the expected bis-NTA-(Ni(2+))(2) exclusively. Instead of the major formation of bis-NTA-(Ni(2+))(2) species, it appears that cyclic discrete 1:1 and 2:2 entities are predominantly formed. It is proposed that these species interact upon ring-opening with multivalent histidine tags. The occurrence of this phenomena accounts for the overall one- to two-order-of-magnitude increase in affinity of ternary complexes involving bis-NTA ligands.

show abstract

“…By doping nitrilotriacetic acid (nta)-modified 5(6)-carboxyfluorescein (F-nta) into PVA film containing Ni 2+ -adsorbed Au NPs, the F-nta formed an H-aggregate, due to the interaction between the nta and Ni 2+ adsorbed on the Au NPs (Schemes 8 and 9). 118 …”

Section: 112mentioning

confidence: 99%

Photochemistry for the Synthesis of Noble Metal Nanoparticles

Sakamoto¹,

Majima²

2010

Bulletin of the Chemical Society of Japan

View full text Add to dashboard Cite

The synthesis of metal nanoparticles is an interdisciplinary subject that is attracting intense research and development owing to the fundamental scientific value of nanometer-sized metal, as well as the broad range of potential applications of metal nanoparticles. Photochemistry plays an important role in various aspects of this research area. It is a unique tool for synthesizing metal nanoparticles, as well as an important technique for investigating the formation mechanism. Metal nanoparticles formation triggered by photochemical reaction is effective for investigating formation mechanisms. We succeeded in observing the formation of metal nanoparticles and clusters by using spectroscopic methods. In addition, single-molecule fluorescent spectroscopy provided important insights into the photochemical reactivity of metal clusters. This photochemical reactivity offers a promising possibility with regard to metal clusters. We developed strategies for constructing metal nanoparticles using a series of photochemical techniques. In situ photochemical synthesis is an effective method for the fabrication of metal nanoparticles, as well as composite materials. A multicolor excitation-induced photochemical reaction resulted in the reduction of metal ions with a highly negative reduction potential, recyclable photosensitization, and 3-D direct laser writing of metal nanoparticles. These new strategies will expand the possible applications of photochemistry for metal nanoparticles synthesis.

show abstract

The Binding Energy of Two Nitrilotriacetate Groups Sharing a Nickel Ion

Cited by 19 publications

References 41 publications

Similarities between heterophilic and homophilic cadherin adhesion

Similarities between heterophilic and homophilic cadherin adhesion

Insight into the Complexation Mode of Bis(nitrilotriacetic acid) (NTA) Ligands with Ni²⁺ Involved in the Labeling of Histidine‐Tagged Proteins

Photochemistry for the Synthesis of Noble Metal Nanoparticles

Contact Info

Product

Resources

About

The Binding Energy of Two Nitrilotriacetate Groups Sharing a Nickel Ion

Cited by 19 publications

References 41 publications

Similarities between heterophilic and homophilic cadherin adhesion

Similarities between heterophilic and homophilic cadherin adhesion

Insight into the Complexation Mode of Bis(nitrilotriacetic acid) (NTA) Ligands with Ni2+ Involved in the Labeling of Histidine‐Tagged Proteins

Photochemistry for the Synthesis of Noble Metal Nanoparticles

Contact Info

Product

Resources

About

Insight into the Complexation Mode of Bis(nitrilotriacetic acid) (NTA) Ligands with Ni²⁺ Involved in the Labeling of Histidine‐Tagged Proteins