The berry constituents quercetin, kaempferol, and pterostilbene synergistically attenuate reactive oxygen species: Involvement of the Nrf2-ARE signaling pathway

Saw, Constance Lay Lay; Guo, Yue; Yang, Anne Yuqing; Paredes-Gonzalez, Ximena; Ramirez, Christina N.; Pung, Douglas; Kong, Ah‐Ng Tony

doi:10.1016/j.fct.2014.07.038

Cited by 210 publications

(125 citation statements)

References 42 publications

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“…These flavonoids are known to possess strong antioxidant activity and are involved in the Nrf2-ARE signaling pathway [140,155]. Our results are consistent with previous reports on antioxidant activity of flavonoids being dependent on the number and position of substituted OH groups and with the presence of sugar residues [140,156].…”

Section: Antioxidant Activitysupporting

confidence: 83%

Structure–activity relationship of immunomodulating pectins from elderberries

Ahmed

Zou

et al. 2015

Carbohydrate Polymers

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Section: Antioxidant Activitysupporting

confidence: 83%

Structure–activity relationship of immunomodulating pectins from elderberries

Ahmed

Zou

et al. 2015

Carbohydrate Polymers

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“…One of the most abundant flavonoids in fruits and vegetables is quercetin (Q), which has been shown to exert anticancer actions, such as the blocking of tumor initiation (17), in addition to exerting anti-oxidative (18) and anti-apoptotic activities in different cancer cell lines (19,20). Notably, Q has the capacity to act either as an antioxidant or as a pro-oxidant, depending on its concentration and the period of exposure (21,22).…”

Section: The Role Of Quercetin and Vitamin C In Nrf2-dependent Oxidatmentioning

confidence: 99%

The role of quercetin and vitamin C in Nrf2-dependent oxidative stress production in breast cancer cells

et al. 2017

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Abstract.The balance between the production and elimination of reactive oxygen species (ROS) is essential in determining whether cells survive or undergo apoptosis. Nuclear factor erythroid 2-related factor 2 (Nrf2) may act as a sensor for electrophilic stress, thus regulating the intracellular antioxidant response. The present study investigated the role of vitamin C (VC) and quercetin (Q) in the induction of Nrf2-mediated oxidative stress in cancer cells. An MTT assay was conducted to examine the anti-proliferative effects of VC and Q. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were performed to determine the messenger RNA (mRNA) and protein expression of Nrf2, respectively. The activity of nicotinamide adenine dinucleotide phosphate dehydrogenase quinone 1, heme oxygenase 1, glutathione peroxidase, glutathione reductase and reduced glutathione were measured by spectrophotometric analysis. Intracellular generation of ROS was determined using 2'-7'-dichlorodihydrofluorescein diacetate fluorescent probes. The results demonstrated that the cytotoxicity (50% inhibitory concentration) of VC and Q were 271.6-480.1 and 155.1-232.9 µM, respectively. Additionally, there was a significant decrease in the expression of Nrf2 mRNA and protein levels following the treatment of breast cancer cells with VC and Q (P=0.024). Following treatment with VC and Q, the nuclear/cytosolic Nrf2 ratio was reduced by 1.7-fold in MDA-MB 231 cells, 2-fold in MDA-MB 468 cells, 1.4-fold in MCF-7 cells and 1.2 fold in A549 cells. Sequential treatment with VC and Q decreased endogenous production of ROS in a dose-dependent manner (P=0.027). The results of the current study suggest that VC and Q treatment may be developed as an adjuvant for patients with cancer and overexpression of Nrf2.

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“…This was clearly noted as in the liver excised from APAP treated mice showing decreased GCLM, GSTa2, NQO1 and HO-1 expression, which was significantly higher in MO leaf extracts administered mice. It has been recently reported that flavonoids such as quercetin, kaempferol from berry synergistically activated the Nrf2-ARE signaling pathway and exhibited anti-oxidative stress activity (Saw et al, 2014). In accordance, the flavonoids such as apigenin, kaempferol, and quercetin present in MO leaves extract are supposedly involved in the induction of synergetic resistance to APAP hepatotoxicity in mice by activation of the Nrf-2-ARE pathway thereby detoxifying and curbing the ROS detrimental effect.…”

Section: Fig 4 -Dose Dependent Effect Of Moringa Oleifera (Mo) Leavementioning

confidence: 93%

The molecular mechanism underlying the hepatoprotective potential of Moringa oleifera leaves extract against acetaminophen induced hepatotoxicity in mice

Karthivashan

Arulselvan

Tan

et al. 2015

Journal of Functional Foods

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The berry constituents quercetin, kaempferol, and pterostilbene synergistically attenuate reactive oxygen species: Involvement of the Nrf2-ARE signaling pathway

Cited by 210 publications

References 42 publications

Structure–activity relationship of immunomodulating pectins from elderberries

Structure–activity relationship of immunomodulating pectins from elderberries

The role of quercetin and vitamin C in Nrf2-dependent oxidative stress production in breast cancer cells

The molecular mechanism underlying the hepatoprotective potential of Moringa oleifera leaves extract against acetaminophen induced hepatotoxicity in mice

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