The Beneficial Effect of Heparin in Preischemic Perfusion Solutions for Cold-Stored Skin Flaps

Pantazi, Glykeria; Knight, Kenneth R.; Romeo, Rosalind; Hurley, James C.; Hennessy, Oliver; Willemart, G.; Penington, Anthony; Morrison, Wayne A.

doi:10.1097/00000637-200044030-00009

Cited by 7 publications

(5 citation statements)

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“…the removal of blood components from the microvasculature during liver retrieval, may affect subsequent graft injury and function [76,77]. Numerous parameters related to graft washout in transplantation settings might influence its efficacy in removing blood cells: (1) type of solution (colloid content, viscosity) [78]; (2) temperature (hypothermic or normothermic washout) [79]; (3) high or low washout pressure/speed (shear stress) [76,80,81]; (4) washout time [77]; (5) heparinization [82,83], and (6) way of washout (portal venous, arterial, or simultaneous) [77]. …”

Section: Technical Issues Concerning P-olt In Ratsmentioning

confidence: 99%

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Czigány

Iwasaki²,

Yagi³

et al. 2015

Eur Surg Res

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Background: Due to a worldwide shortage of donor organs for liver transplantation, alternative approaches, such as split and living donor liver transplantations, were introduced to increase the donor pool and reduce mortality on liver transplant waiting lists. Numerous details concerning the mechanisms and pathophysiology of liver regeneration, small-for-size syndrome, rejection, and tolerance in partial liver transplantation facilitated the development of various animal models. The high number of preclinical animal studies contributed enormously to our understanding of many clinical aspects of living donor and partial liver transplantations. Summary: Microsurgical rat models of partial orthotopic liver transplantation are well established and widely used. Nevertheless, several issues regarding this procedure are controversial, not clarified, or not yet properly standardized (graft rearterialization, size reduction techniques, etc.). The major aim of this literature review is to give the reader a current overview of rat orthotopic liver transplantation models with a special focus on partial liver transplantation. The aspects of model evolution, microsurgical training, and different technical problems are analyzed and discussed in detail. Our further aim in this paper is to elaborate a detailed publication guide in order to improve the quality of reporting in the field of rat liver transplantation according to the ARRIVE guidelines and the 3R principle. Key Messages: Partial orthotopic liver transplantation in rats is an indispensable, reliable, and cost-efficient model for transplantation research. A certain consensus on different technical issues and a significant improvement in scientific reporting are essential to improve transparency and comparability in this field as well as to foster refinement.

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Section: Technical Issues Concerning P-olt In Ratsmentioning

confidence: 99%

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Czigány

Iwasaki²,

Yagi³

et al. 2015

Eur Surg Res

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“…The cannula is pulled out after 2-3 days of stopping the infusion. People have described salvage of microsurgical flaps with leeches, [35] thrombolytic therapy, [36,37] external shunts, [38] but we do not have any experience of these methods. Figure 4 shows a case where early exploration saved the flap.…”

Section: Vascular Compromise and Re-explorationmentioning

confidence: 99%

Unfavourable results in free tissue transfer

Koul

Patil

Nahar

2013

Indian J Plast Surg

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With recent advances in the instrumentation and with increased expertise the results of microvascular surgery are getting better. Complications though, cannot be completely avoided. This paper gives a brief introduction to the possible complications at various stages of free tissue transfer. With careful planning and execution and vigilant postoperative care the overall success rate can be improved.

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“…An ϳ9-cm 2 area was marked over the groin, and the SIE EMB was dissected based on the femoral vessels. Prior to detachment from the systemic circulation, 1 ml of heparinized saline (10 U/ml) (24) was flushed through the SIE EMB via the femoral artery. The EMB, comprising skin, adipose tissue, and its supporting vasculature, was detached with maximal femoral vessel pedicle length; transferred to our bioreactor cassette, where the vessels were cannulated onto our custom bioreactor system (Harvard Apparatus, Holliston, MA, USA); and secured using 9-0 nylon sutures.…”

Section: Emb Harvestmentioning

confidence: 99%

Tissue engineering using autologous microcirculatory beds as vascularized bioscaffolds

et al. 2008

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Classic tissue engineering paradigms are limited by the incorporation of a functional vasculature and a reliable means for reimplantation into the host circulation. We have developed a novel approach to overcome these obstacles using autologous explanted microcirculatory beds (EMBs) as bioscaffolds for engineering complex three-dimensional constructs. In this study, EMBs consisting of an afferent artery, capillary beds, efferent vein, and surrounding parenchymal tissue are explanted and maintained for 24 h ex vivo in a bioreactor that preserves EMB viability and function. Given the rapidly advancing field of stem cell biology, EMBs were subsequently seeded with three distinct stem cell populations, multipotent adult progenitor cells (MAPCs), and bone marrow and adipose tissue-derived mesenchymal stem cells (MSCs). We demonstrate MAPCs, as well as MSCs, are able to egress from the microcirculation into the parenchymal space, forming proliferative clusters. Likewise, human adipose tissue-derived MSCs were also found to egress from the vasculature and seed into the EMBs, suggesting feasibility of this technology for clinical applications. We further demonstrate that MSCs can be transfected to express a luciferase protein and continue to remain viable and maintain luciferase expression in vivo. By using the vascular network of EMBs, EMBs can be perfused ex vivo and seeded with stem cells, which can potentially be directed to differentiate into neo-organs or transfected to replace failing organs and deficient proteins.

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The Beneficial Effect of Heparin in Preischemic Perfusion Solutions for Cold-Stored Skin Flaps

Cited by 7 publications

References 0 publications

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Improving Research Practice in Rat Orthotopic and Partial Orthotopic Liver Transplantation: A Review, Recommendation, and Publication Guide

Unfavourable results in free tissue transfer

Tissue engineering using autologous microcirculatory beds as vascularized bioscaffolds

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