The Beneficial Effect of Coenzyme Q10 and Lipoic Acid on Obstructive Bladder Dysfunction in the Rabbit

Juan, Yung‐Shun; Levin, Robert M.; Chuang, Shu Mien; Hydery, Tasmina; Li, Suning; Kogan, Barry A.; Schuler, Catherine; Huang, Chun‐Hsiung; Mannikarottu, Anita

doi:10.1016/j.juro.2008.07.022

Cited by 24 publications

(22 citation statements)

References 20 publications

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“…Juan et al conducted a study in 2008 with rabbits and reported increased bladder weights after four and seven weeks of BOO. The authors demonstrated that the groups undergoing αlipoic acid and coenzyme Q10 treatment had significantly reduced bladder weights when compared with those of the 7-week obstruction group (16). …”

Section: Discussionmentioning

confidence: 99%

Effect of αlipoic acid and silymarin on bladder outlet obstruction

Yıldırım

Başeskioğlu

Temel

et al. 2012

Experimental and Therapeutic Medicine

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The aim of the present study was to determine whether the treatment of obstructed rat bladders with αlipoic acid (ALA) and silymarin reverses the biochemical and physiological responses to bladder outlet obstruction (BOO). A total of 32 adult Sprague Dawley rats were divided into four groups (n=8 per group): sham (placebo surgery) animals with no treatment (group 1); control animals with surgically induced BOO (group 2); obstructed rats treated with ALA (group 3); and obstructed rats treated with silymarin (group 4). Histological evaluation, bladder weights, collagen structure, TdT-mediated biotin nick end-labeling (TUNEL), inducible nitric oxide sentase (iNOS) mRNA levels, malondialdehyde (MDA) levels and tumor necrosis factor (TNF) levels were investigated. The ALA-treated group had similar bladder weights, collagen levels and TUNEL positivity and decreased iNOS levels compared with the control group, while the silymarin group exhibited further differences. Serum MDA and TNF-α levels were both decreased in the ALA and silymarin groups. ALA treatment reduced the increased oxidative stress and bladder inflammation caused by BOO and may contribute to the protection of bladder function.

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Section: Discussionmentioning

confidence: 99%

Effect of αlipoic acid and silymarin on bladder outlet obstruction

Yıldırım

Başeskioğlu

Temel

et al. 2012

Experimental and Therapeutic Medicine

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“…Partial outlet obstruction and in vivo models of ischemia have marked deleterious effects on superoxide dismutase and catalase, and as stated above have also been demonstrated to produce significant oxidative damage (10,(12)(13)(14). A variety of antioxidants and natural products that show significant antioxidant properties have been shown to protect the rabbit urinary bladder from contractile, cellular, and subcellular damage and dysfunction mediated by both partial outlet obstruction, and bilateral ischemia / reperfusion (15)(16)(17)(18)(19)(20). One of the most potent of these agents has been shown to be a grape suspension made from whole grapes (13,21,22).…”

Section: Introductionmentioning

confidence: 92%

Effect of hydrogen peroxide on rabbit urinary bladder citrate synthase activity in the presence and absence of a grape suspension

et al. 2010

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Purpose: The etiology of obstructive bladder dysfunction includes free radical damage to mitochondria. Feeding rabbits a standardized grape suspension protects the ability of the bladder to contract and empty in part by preventing mitochondrial damage, thus maintaining smooth muscle and mucosal metabolism. The objective of the current study is to determine the direct effect of this grape suspension on the response of mitochondria to the oxidative effects of hydrogen peroxide. Materials and Methods: Six male rabbits were anesthetized with sodium pentobarbital and the bladders excised. Four full thickness strips were obtained for contractile studies and the balance separated into smooth muscle and mucosa compartments by blunt dissection. The effect of hydrogen peroxide on the contractile response to field stimulation was quantitated. Each tissue was homogenized and the effects of increasing concentrations of hydrogen peroxide in the presence and absence of grape suspension on citrate synthase activity was determined. Results: Citrate synthase activity was significantly higher in the mucosa than in the muscle. The grape suspension had no effect on control citrate synthase activity. However, the grape suspension provided significant protection of both smooth muscle and mucosal citrate synthase activity. Conclusions: These studies support the conclusion that the grape suspension provides direct protection of mitochondrial function.

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“…In virtually all of these oxidative stress-linked dysfunctions, antioxidants have proven to be very valuable in their treatment [13,[16][17][18][19]. In addition to specific antioxidants such as coenzyme Q10, alpha lipoic acid and vitamin E, certain natural products have been shown to be effective treatments in regard to these specific diseases [20][21][22][23][24].…”

Section: Introductionmentioning

confidence: 99%

“…The urinary bladder is a relatively closed system: that is, I/R can be induced in the bladder with little or no damage to other organ systems [31,32]. Antioxidants have been proven to be very effective in protecting the rabbit bladder from oxidative damage mediated by OBD [16,33].OBD, induces a variety of bladder dysfunctions in addition to I/R including hypertrophy, hyperplasia, apoptosis, and inflammation. In order to eliminate these other effects, we have developed in-vivo and invitro models of bladder I/R [19,34].…”

Section: Introductionmentioning

confidence: 99%

Effects of Ganoderma Lucidum on Biochemical Dysfunctions of the Rabbit Urinary Bladder using an In-Vitro Model of Ischemia / Reperfusion

Levin¹,

Li²,

Wu³

et al. 2017

SDRP-JPS

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ABSTRACT:Background: Oxidative stress involving ischemia followed by reperfusion are major etiological factors in obstructive bladder dysfunction in both men and rabbits. Specific natural products such as Ganoderma lucidum with significant antioxidant activity have proven to be useful in their treatment. In two recent studies we demonstrated that pretreatment with Ganoderma was effective preventing bladder dysfunction using both invivo and in-vitro models of ischemia-reperfusion. The current study is a follow-up study using the in-vitro model of ischemia-reperfusion. Methods: Eight New Zealand White rabbits were divided into 2 groups. One group (Group-1) was fed Ganoderma (100 mg/Kg) daily for 3 weeks while the other group (Group-2) were given saline. At the end of a 3 week period, each rabbit was euthanized and the bladder separated into twelve full thickness strips and mounted in individual baths. After 1 hour in oxygenated Tyrodes with glucose, 4 strips from each group were removed frozen and stored at -80 o C. At this time, the oxygenated Tyrodes + glucose were changed to Tyrodes equilibrated with nitro gen in the absence of glucose (ischemia) for 1 hour. After 1 hour of ischemia the buffer was then changed back to normal oxygenated Tyrode's with glucose and allowed to recover for 2 hours (reperfusion). After this time period, the four remaining strips from each group were frozen and stored. The isolated strips were analyzed for the following biomarker enzymes: citrate synthase (mitochondria), calcium ATPase (intracellular calcium movement through the cell wall), and sarcoendoplasmic reticular ATPase (intracellular calcium uptake into the sarcoplasmic reticulum following stimulation. Results: Ischemia-reperfusion resulted in a significant decrease (~50%) in all three enzyme activities. Pretreating with Ganoderma protected all three enzyme activities which were approximately at control levels. Conclusion: Pretreatment of rabbits for three weeks with Ganoderma prior to subjecting them to invitro ischemia-reperfusion significantly protected the bladder enzymes.

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The Beneficial Effect of Coenzyme Q10 and Lipoic Acid on Obstructive Bladder Dysfunction in the Rabbit

Abstract: The current study clearly demonstrates that coenzyme Q10 and alpha-lipoic acid supplementation can improve bladder function after outlet obstruction.

Cited by 24 publications

References 20 publications

Effect of αlipoic acid and silymarin on bladder outlet obstruction

Effect of αlipoic acid and silymarin on bladder outlet obstruction

Effect of hydrogen peroxide on rabbit urinary bladder citrate synthase activity in the presence and absence of a grape suspension

Effects of Ganoderma Lucidum on Biochemical Dysfunctions of the Rabbit Urinary Bladder using an In-Vitro Model of Ischemia / Reperfusion

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