The Behavior of Embryonic Neural Cells within the 3D Micro-structured Collagen-Based Scaffolds

Pietrucha, Krystyna; Szymański, Jacek; Drobnik, Jacek

doi:10.1007/978-3-319-11128-5_137

Cited by 4 publications

(15 citation statements)

References 14 publications

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“…Earlier studies indicated that the secondary triple helix structure of collagen present within Col scaffolds remained unchanged, thus allowing the optimal biological properties of the scaffold to be used in regenerative medicine. [3][4][5][6] In the present study, the presence or absence of cross-links, CS and HA in the collagen sponges had a significant effect on the overall structure and distribution of the interconnected pores (Figs. 1, 2).…”

Section: Discussionmentioning

confidence: 48%

“…4) and astrocytes (GFAP positive cells, data not shown). 6 Fibroblasts were able to enter both Col and Col + CS + + HA scaffolds, but the Col + CS + HA scaffolds were less populated on the upper surface. Statistically signifi- Fig.…”

Section: Resultsmentioning

confidence: 96%

“…As noted above, the addition of hyaluronic acid to an earlier investigated scaffold (Col + CS) created a tri-copolymer (Col + CS + HA), the potential of which for embryonic nerve cell and wound fibroblast culture had not been investigated prior to the present study. 6 The porosity of each of the matrices was characterized using a Nova NanoSEM 230 scanning electron microscope (FEI Company, Eindhoven, the Netherlands) with an Apollo 40 SDDEDS X-ray microanalyser (EDAX McKee Drive Mahwah, USA). The operating parameters were as follows: A low vacuum setting of 70 Pa, an accelerating voltage of 10 kV and LVD detector selected.…”

Section: Preparation and Characterization Of Ternary Composite Collagmentioning

confidence: 99%

“…2 The favorable physical and chemical properties of collagenous (Col) scaffolds for regenerative medicine have been described by Pietrucha et al [3][4][5][6] The Col materials create a supportive microenvironment for fibroblasts, osteoblasts and embryonic nerve cells. 2,[6][7][8] Wound fibroblasts are thought to enter the scaffold from the margins of the wound and participate in scar formation. Collagen may regulate the function of cells in the wound by binding to the integrin subfamily (α1β2, α2β2, α10β2 and α11β2), which would influence granulation tissue remodeling, angiogenesis, fibroblast adhesion and myofibroblast differentiation in the wound.…”

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confidence: 99%

“…A previous study found that a scaffold composed of collagen cross-linked with CS constitutes a good environment for embryonic nerve cells. 6 A novel element of the present study was that it investigated whether a collagen tri-copolymer with the addition of HA and CS (Col + HA + CS) can enhance cultures not only of embryonic nerve cells, but also wound fibroblasts. In this way, the study determined the usefulness of this tri-copolymer for tissue engineering.…”

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Collagenous scaffolds supplemented with hyaluronic acid and chondroitin sulfate used for wound fibroblast and embryonic nerve cell culture

et al. 2017

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Background. Tissue engineering is a strategy aimed at improving the regeneration of injured tissues.Objectives. The aim of the present study was to determine whether a tri-copolymer composed of crosslinked collagen, chondroitin sulfate and hyaluronic acid (Col + CS + HA) provides a better environment for fibroblast and embryonic nerve cell culture than a collagenous scaffold (Col).Material and methods. The porosity of each of the matrices was characterized with a scanning electron microscope. Fibroblasts were isolated from rat wound granulation tissue (polypropylene net implanted subcutaneously). Embryonic nerve cells were obtained from the brains of rat embryos. The cells were applied to scaffolds and then stained with bisbenzimide to calculate cell entrapment within the material. The metabolic activity of the cells cultured within the scaffolds was tested using the 3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay.Results. The Col scaffolds had a homogenously porous structure with a pore diameter of 50 μm for 70% of pores. The pore diameter in the tri-copolymer (Col + HA + CS) ranged from 24 to 160 μm (95% of total pore volume). Four times more cells (fibroblasts and embryonic nerve cells) were trapped within the superficial part of the collagenous scaffold than that of the tri-copolymer. On the third day of culture the metabolic activity of the fibroblasts within the 2 tested scaffolds was significantly higher than in the control conditions (cell culture on a laminin-coated surface). Also, the embryonic nerve cells demonstrated increased metabolic activity in Col + CS + HA scaffolds than the Col scaffolds.Conclusions. Both fibroblasts and embryonic nerve cells could be seeded within the 2 tested scaffolds. Both the scaffolds provide good conditions for fibroblast culture. However, the Col + CS + HA tri-copolymer is preferable for embryonic nerve cell engineering.

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Section: Discussionmentioning

confidence: 48%

Section: Resultsmentioning

confidence: 96%

Section: Preparation and Characterization Of Ternary Composite Collagmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Collagenous scaffolds supplemented with hyaluronic acid and chondroitin sulfate used for wound fibroblast and embryonic nerve cell culture

et al. 2017

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Comparison of various types of collagenous scaffolds applied for embryonic nerve cell culture

et al. 2017

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Best of Both Hydrogel Worlds: Harnessing Bioactivity and Tunability by Incorporating Glycosaminoglycans in Collagen Hydrogels

Walimbe

Panitch

2020

Bioengineering

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Collagen, the most abundant protein in mammals, has garnered the interest of scientists for over 50 years. Its ubiquitous presence in all body tissues combined with its excellent biocompatibility has led scientists to study its potential as a biomaterial for a wide variety of biomedical applications with a high degree of success and widespread clinical approval. More recently, in order to increase their tunability and applicability, collagen hydrogels have frequently been co-polymerized with other natural and synthetic polymers. Of special significance is the use of bioactive glycosaminoglycans—the carbohydrate-rich polymers of the ECM responsible for regulating tissue homeostasis and cell signaling. This review covers the recent advances in the development of collagen-based hydrogels and collagen-glycosaminoglycan blend hydrogels for biomedical research. We discuss the formulations and shortcomings of using collagen in isolation, and the advantages of incorporating glycosaminoglycans (GAGs) in the hydrogels. We further elaborate on modifications used on these biopolymers for tunability and discuss tissue specific applications. The information presented herein will demonstrate the versatility and highly translational value of using collagen blended with GAGs as hydrogels for biomedical engineering applications.

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The Behavior of Embryonic Neural Cells within the 3D Micro-structured Collagen-Based Scaffolds

Cited by 4 publications

References 14 publications

Collagenous scaffolds supplemented with hyaluronic acid and chondroitin sulfate used for wound fibroblast and embryonic nerve cell culture

Collagenous scaffolds supplemented with hyaluronic acid and chondroitin sulfate used for wound fibroblast and embryonic nerve cell culture

Comparison of various types of collagenous scaffolds applied for embryonic nerve cell culture

Best of Both Hydrogel Worlds: Harnessing Bioactivity and Tunability by Incorporating Glycosaminoglycans in Collagen Hydrogels

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