Comparison of various types of collagenous scaffolds applied for embryonic nerve cell culture

Drobnik, Jacek; Pietrucha, Krystyna; Kudzin, Marcin H.; Mader, K.; Szymański, Jacek; Szczepanowska, A

doi:10.1016/j.biologicals.2017.01.001

Cited by 6 publications

(5 citation statements)

References 29 publications

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“…The scaf- folds Col-CS and Col-DAC, crosslinked by EDC and DAC, respectively, were shown to immobilize hiP-SC-NPs to the active biofunctional surface and allow for their further commitment into three neural lineages: neuronal, astrocytic and oligodendroglial. This is consistent with the study of Drobnik et al [6] that collagen-based scaffolds using different cross-linking methods were shown to be suitable in elaborateing environments for primary nerve cells isolated from murine embryonic brains.…”

Section: Discussionsupporting

confidence: 92%

Functional properties of different collagen scaffolds to create a biomimetic niche for neurally committed human induced pluripotent stem cells (iPSC)

Pietrucha¹,

Zychowicz²,

Podobińska³

et al. 2017

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Section: Discussionsupporting

confidence: 92%

Functional properties of different collagen scaffolds to create a biomimetic niche for neurally committed human induced pluripotent stem cells (iPSC)

Pietrucha¹,

Zychowicz²,

Podobińska³

et al. 2017

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“…Collagen is a good medium for cell and drug delivery [66] and is sufficiently flexible for nerve conduits with physical features tailored for different sections of the nerve pathway [67]. In addition, it can support topographical cues that allow axonal regrowth and facilitate celladhesion, survival, and migration along different nerve tract domains [68]. Such collagen nerve conduits have been demonstrated to support nerve regeneration and re-innervation of muscle [69].…”

Section: Collagenmentioning

confidence: 99%

Bio-Scaffolds as Cell or Exosome Carriers for Nerve Injury Repair

Poongodi

Chen

Yang

et al. 2021

IJMS

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Central and peripheral nerve injuries can lead to permanent paralysis and organ dysfunction. In recent years, many cell and exosome implantation techniques have been developed in an attempt to restore function after nerve injury with promising but generally unsatisfactory clinical results. Clinical outcome may be enhanced by bio-scaffolds specifically fabricated to provide the appropriate three-dimensional (3D) conduit, growth-permissive substrate, and trophic factor support required for cell survival and regeneration. In rodents, these scaffolds have been shown to promote axonal regrowth and restore limb motor function following experimental spinal cord or sciatic nerve injury. Combining the appropriate cell/exosome and scaffold type may thus achieve tissue repair and regeneration with safety and efficacy sufficient for routine clinical application. In this review, we describe the efficacies of bio-scaffolds composed of various natural polysaccharides (alginate, chitin, chitosan, and hyaluronic acid), protein polymers (gelatin, collagen, silk fibroin, fibrin, and keratin), and self-assembling peptides for repair of nerve injury. In addition, we review the capacities of these constructs for supporting in vitro cell-adhesion, mechano-transduction, proliferation, and differentiation as well as the in vivo properties critical for a successful clinical outcome, including controlled degradation and re-absorption. Finally, we describe recent advances in 3D bio-printing for nerve regeneration.

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“…Collagen is a very good vehicle for drug and cell delivery ( Dalamagkas et al, 2016 ) and it is possible to modify its physical features along different parts of the same conduit ( Aigner et al, 2020 ). The different binding domains on it give the possibility to create topographical cues that guide the axon regrowth ( Stang et al, 2005a , b ) facilitating cell adhesion, survival and migration ( Alberti et al, 2014 ; Sulong et al, 2014 ; Drobnik et al, 2017a , b ). Many researchers have shown that collagen has biological properties superior to other materials available in the market for nerve scaffold fabrication and it is also effective over a nerve gap distance of at least 15 mm ( Archibald et al, 1991 ).…”

Section: Natural-based Biomaterialsmentioning

confidence: 99%

Natural-Based Biomaterials for Peripheral Nerve Injury Repair

Fornasari

Carta

Gambarotta

et al. 2020

Front. Bioeng. Biotechnol.

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Peripheral nerve injury treatment is a relevant problem because of nerve lesion high incidence and because of unsatisfactory regeneration after severe injuries, thus resulting in a reduced patient's life quality. To repair severe nerve injuries characterized by substance loss and to improve the regeneration outcome at both motor and sensory level, different strategies have been investigated. Although autograft remains the gold standard technique, a growing number of research articles concerning nerve conduit use has been reported in the last years. Nerve conduits aim to overcome autograft disadvantages, but they must satisfy some requirements to be suitable for nerve repair. A universal ideal conduit does not exist, since conduit properties have to be evaluated case by case; nevertheless, because of their high biocompatibility and biodegradability, natural-based biomaterials have great potentiality to be used to produce nerve guides. Although they share many characteristics with synthetic biomaterials, natural-based biomaterials should also be preferable because of their extraction sources; indeed, these biomaterials are obtained from different renewable sources or food waste, thus reducing environmental impact and enhancing sustainability in comparison to synthetic ones. This review reports the strengths and weaknesses of natural-based biomaterials used for manufacturing peripheral nerve conduits, analyzing the interactions between natural-based biomaterials and biological environment. Particular attention was paid to the description of the preclinical outcome of nerve regeneration in injury repaired with the different natural-based conduits.

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Comparison of various types of collagenous scaffolds applied for embryonic nerve cell culture

Cited by 6 publications

References 29 publications

Functional properties of different collagen scaffolds to create a biomimetic niche for neurally committed human induced pluripotent stem cells (iPSC)

Functional properties of different collagen scaffolds to create a biomimetic niche for neurally committed human induced pluripotent stem cells (iPSC)

Bio-Scaffolds as Cell or Exosome Carriers for Nerve Injury Repair

Natural-Based Biomaterials for Peripheral Nerve Injury Repair

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