The bar gene has selectable and screenable marker in plant engineering

D’Halluin, Kathleen; Block, Marc De; Denecke, Jürgen; Janssens, Jan; Leemans, J.; Reynaerts, A.; Botterman, Johan

doi:10.1016/0076-6879(92)16038-l

Cited by 106 publications

(11 citation statements)

References 28 publications

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“…Phosphinothricin (PT) is used as the active ingredient in several widely used commercial herbicide formulations sold by Bayer including Liberty ® , Basta ® , and Rely ® . The genes encoding for bialaphos resistance (phosphinothricin acetyltransferase), originally cloned from bialaphos producing streptomycetes [63,56,59,3], have been used as selection markers in plant biology and engineering [11]. Several PT resistant varieties of canola, corn, cotton, and soybeans have been developed by Bayer and marketed as LibertyLink ® crops for use in combination with their commercial PT, or glufosinate, herbicides for weed management.…”

Section: The Discovery Of Phosphonate Natural Product Antibioticsmentioning

confidence: 99%

Genomics-enabled discovery of phosphonate natural products and their biosynthetic pathways

Doroghazi

Metcalf

2014

Journal of Industrial Microbiology and Biotechnology

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Phosphonate natural products have proven to be a rich source of useful pharmaceutical, agricultural and biotechnology products, whereas study of their biosynthetic pathways has revealed numerous intriguing enzymes that catalyze unprecedented biochemistry. Here we review the history of phosphonate natural product discovery, highlighting technological advances that have played a key role in the recent advances in their discovery. Central to these developments has been the application of genomics, which allowed discovery and development of a global phosphonate metabolic framework to guide research efforts. This framework suggests that the future of phosphonate natural products remains bright, with many new compounds and pathways yet to be discovered.

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Section: The Discovery Of Phosphonate Natural Product Antibioticsmentioning

confidence: 99%

Genomics-enabled discovery of phosphonate natural products and their biosynthetic pathways

Doroghazi

Metcalf

2014

Journal of Industrial Microbiology and Biotechnology

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“…Conventional selection for BASTA resistance (conferred by bar , the phosphinothricin N-acetlytransferase gene) [26] involves spraying the aerial parts of germinated seedlings and can be performed in non-sterile conditions with seeds sown directly on soil at relatively high densities. The disadvantage of this approach is the time required to identify positive transformants: typically at least three spray applications are spread across three weeks [27].…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial solid media for screening non-sterile Arabidopsis thaliana seeds

Behrendorff

Borràs-Gas

Pribil

2019

Preprint

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7 Background 8 Stable genetic transformation of plants is a low-efficiency process, and identification of 9 positive transformants usually relies on screening for expression of a co-transformed marker 10 gene. Often this involves germinating seeds on solid media containing a selection reagent. 11 Germination on solid media requires surface sterilization of seeds and careful aseptic 12 technique to prevent microbial contamination, but surface sterilization techniques are time 13 consuming and can cause seed mortality if not performed carefully. We developed an 14antimicrobial cocktail that can be added to solid media to inhibit bacterial and fungal growth 15 without impairing germination, allowing us to bypass the surface sterilization step. 16

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“…The most accepted marker gene has been npt II, encoding resistance to aminoglicosidic antibiotics (neomicin/kanamicin). The bla gene, encoding ampicilin resistance, and the bar gene, encoding phosphinothricin tolerance, have also been widely employed (D ' Halluin et al 1992 ). Therefore, most of the screening methods are based on the detection of P -35S, T -nos , and bla and npt II genes.…”

Section: Analysis Of Regulatory Sequences and Marker Genesmentioning

confidence: 99%