A high content of Vitamin C (over 100 mg/100 g) in three samples of honey from a mountainous area (Damavand) in Iran is reported. The vitamin was determined by thin layer chromatography of its 2, 4 dinitro phenyl osazone, as well as by the less specific 2,6 dichlorophenol indophenol method. The effect of one of these samples on the prevention and cure of scurvy in the guinea pig indicates that it contained compounds with vitamin C activity.