Multifloral honey of giant honey bee, A. florea (wild) and Indian hivebee, A. cerana indica (apiary) was collected from the plains, hills and western ghats of Karnataka and vitamin characteristics were determined during April 2021 to March 2022. Six vitamins viz., thiamine (B1), riboflavin (B2), niacin (B3), panthothenic acid (B5), pyridoxine (B6) and ascorbic acid (C) were analyzed in honey of wild and apiary honeybees. B1 (0.07 µ gms) and B6 (1.80 µ gms) of A.florea honey was minimum and maximum from hills and western ghats respectively. Similarly A.cerana honey had lowest of B5 (0.08 µ gms) and highest of B6 (1.62 µ gms) from hills and western ghats respectively. B2 (0.12 µ gms -0.20 µ gms), C (1.32 µ gms -1.55 µ gms) and B3 (0.43 µ gms -0.55 µ gms) of honey demonstrated variations in wild and apiary honeybees. The vitamin C and B6 of honey was significant at 1% (p<0.01) level in plains, hills and western ghats while B1, B2, B3 and B5 of honey was not significant at 1% (p<0.01) level in plains, hills and western ghats of Karnataka. All the six vitamins tested in A.florea and A.cerana displayed quantitative fluctuations in different geographical areas which are discussed in ensuing paper.