The B*4002 allele encodes the B61 antigen: B40* is identical to B61

Domena, John D.; Johnston-Dow, L; Parham, Peter

doi:10.1111/j.1399-0039.1992.tb02053.x

Cited by 29 publications

(7 citation statements)

References 15 publications

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“…The transfected cells were used as a source for B*4801 molecules, which were purified using the MB40.3 monoclonal antibody. This mouse monoclonal antibody was shown previously to bind strongly to HLA-B7 and HLA-B60 (B*4001), less well to HLA-B61 (B*4002) (21) and weakly to HLA-B27 (19,22). Comparison of the amino-acid sequences of HLA-B heavy chains suggested the sequence motif of aspartate 177, lysine 178 and glutamate 180 at the carboxyl-terminal end of the a2 domain is important for strong MB40.3 binding (23).…”

Section: '4801 Peptide-binding Motifmentioning

confidence: 86%

Interactions of HLA‐B*4801 with peptide and CD8

et al. 1997

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Functional properties of the B*4801 allotype were investigated using HLA class I-deficient 221 cells transfected with B*4801 cDNA. From pool sequence analysis of endogenously bound peptides, B*4801 was shown to select for nonamer peptides having glutamine or lysine at position 2 and leucine at the carboxyl-terminus. In an in vitro cell-cell binding assay, B*4801 binds CD8 alpha homodimers weakly due to the presence of a threonine residue at position 245 in the alpha 3 domain. A mutant B*4801 molecule in which alanine replaces threonine 245, binds CD8 alpha homodimers at levels comparable to those of other HLA class I allotypes. Despite the low affinity of B*4801 for CD8 alpha, alloreactive T-cells that recognize B*4801 molecules expressed by the 221 transfectant are inhibited by anti-CD8 monoclonal antibodies. Analysis of 25 B*48-expressing individuals from various populations showed threonine 245 was encoded by every B*48 allele.

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Section: '4801 Peptide-binding Motifmentioning

confidence: 86%

Interactions of HLA‐B*4801 with peptide and CD8

et al. 1997

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“…lished sequences (5, 7, [18][19][20][21]. In the analysis reported here we compare the results of typing the HLA-B alleles of the KT17 and OLGA cells by cloning and sequencing full-length cDNA with those obtained by the ,newer SBT method described by Santamaria et al (7).…”

Section: Discussionmentioning

confidence: 99%

A small test of a sequence‐based typing method: Definition of the B*1520 allele

et al. 1994

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Santamaria et al. (Human Immunology 1993 37: 39-50) describe a method of sequence-based typing (SBT) for HLA-A, B and C alleles said to give "unambiguous typing of any sample, heterozygous or homozygous, without requiring additional typing information". From SBT analysis, which involves determination of partial sequences of mixed alleles, these investigators reported that cell lines KT17 (HLA-B35,62) and OLGA (HLA-B62) from the reference panel of the 10th International Histocompatibility Workshop express novel variants of HLA-B15 (B1501-MN6) and HLA-B35 (B3501-MN7) respectively. To study further the novel alleles, we cloned and sequenced full-length HLA-B cDNA clones isolated from the KT17 and OLGA cell lines. We find that KT17 expresses B*3501, as assigned by SBT, and B*1501, the common allele encoding the B62 antigen. We were unable to confirm that KT17 expresses the novel B1501-MN6 variant identified by SBT. For OLGA our analysis confirms the partial sequences obtained by SBT. Thus OLGA expresses B*1501 and a novel HLA-B allele. The complete sequence of the latter shows it is a hybrid having exons 1 and 2 in common with B*1501 and other B15 subtypes and exons 3-7 in common with B*3501 and related molecules including B*5301 and B*5801. The novel allele has been designated B*1520 because of its sequence similarity with the B15 group; furthermore, serological analysis shows that the B*1520 product does not express epitopes in common with either B35, B53 or B58. The B*1520 heavy chain has a similar isoelectric point to A*3101; B*1520 was undetected by previous applications of isoelectric focusing because B*1520 and A31 are both expressed by OLGA. In conclusion, HLA-B typing of two cell lines by cDNA cloning and sequencing gives concordant results with SBT for three of the four alleles. The cause of the discrepancy for the fourth allele is unknown, however, this finding indicates that the novel HLA-A, B and C sequences emerging from SBT studies need independent verification.

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“…Isolation of mRNA, amplification of HLA-B cDNA and subcloning were as described (7, 8). Sequencing was performed using an Applied Biosystems automated DNA sequencer (9). The nucleotide sequence of B*5901 has been submitted to the EMBL/ GenBank Databases under accession number L07743.…”

Section: Methodsmentioning

confidence: 99%