Primary structure shows HLA‐B59 to be a hybrid of HLA‐B55 and HLA‐B51, and not a subtype of HLA‐B8

Hildebrand, William H.; Domena, John D.; Parham, Peter

doi:10.1111/j.1399-0039.1993.tb02001.x

Cited by 14 publications

(6 citation statements)

References 15 publications

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“…26 The alleles belonging to these specificities cluster in all 3 0 introns together with B*5901. Since with regard to exonic sequences, B*5901 is identical to B*5502 except within codons 67-83 of the a1 helix where it is identical to B*51, 27 this finding gives a further phylogenetic support for the B22 group in their 3 0 part.…”

Section: Resultsmentioning

confidence: 72%

The nature of introns 4–6 suggests reduced lineage specificity in HLA-B alleles

Baengeroth

Blasczyk

2003

Genes Immun

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For most HLA-B alleles, coding sequences of the 3 0 part of the genes still need to be determined, and sequences of the 3 0 noncoding regions have yet to be studied systematically. In this study, we have determined the sequences of introns 4-6 in all HLA-B allelic groups, and computed nucleotide substitution rates and phylogenetic relationships. These sequences demonstrated an inconsistent pattern of intralineage specificity, intralineage diversity, and interlineage diversity that is best characterized by a patchwork pattern. Apart from phylogenetic studies about HLA diversity and diversification, the sequence data obtained in our study may prove valuable for haplotype-specific sequencing of the 3 0 part of HLA-B and for the explanation of recombination events in newly described HLA-B alleles.

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Section: Resultsmentioning

confidence: 72%

The nature of introns 4–6 suggests reduced lineage specificity in HLA-B alleles

Baengeroth

Blasczyk

2003

Genes Immun

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“…The aim of the HLA-B gene low-resolution DNA typing primer panel was to develop a system which would amplify, in a single PCR round, a comprehensive set of HLA-B alleles. These are based on the Class I nucleotide sequences published by Zemmour & Parham (4), including more recently published alleles (34,17,35,38) so that all the HLA-B serological specificities were covered. Class I antigens fall into serologically characterized cross-reactive groups and these can be sometimes explained when looking at nucleotide sequences as being due to shared motifs between different alleles.…”

Section: Discussionmentioning

confidence: 99%

“…The primer pairs therefore amplified a region from exon 2 to exon 3 including the intron of 240 bp in length. Primers were synthesized "in house" and their design was based on published nucleotide sequences (4,34,17,35). Their sequences are given in Table 1.…”

Section: Amplification Primersmentioning

confidence: 99%

Low‐resolution DNA typing for HLA‐B using sequence‐specific primers in allele‐ or group‐specific ARMS/PCR

Sadler¹,

Petronzelli

Krausa

et al. 1994

Tissue Antigens

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The products of the human major histocompatibility complex (HLA Class I and II) have historically been detected using serological or cellular assays. With the availability of DNA sequence information for alleles of the HLA system, and with the development of molecular biological techniques it has become possible to tissue type for allelic differences in the HLA genes themselves. We describe here a polymerase chain reaction (PCR) system, based on the principle of the amplification refractory mutation system (ARMS), for low-resolution DNA typing of the HLA-B gene. The technique involves a one-step PCR from genomic DNA using sequence-specific primers in particular combinations that determine the specificity of each reaction. A low-resolution primer panel has been designed, based on published HLA-B gene nucleotide sequences, consisting of 34 sequence-specific primers (SSP) in 24 PCR reactions which cover all known HLA-B alleles, to give allele-specific or group-specific amplification of DNA fragments of defined size (344-784bp). Advantages of the system are that it can be performed in under 4 hours including DNA extraction, results are easy to interpret and it does not require viable cells.

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“…Further identification of critical residues determining HLA‐A 16–18 and ‐B 19–29 serotypes and epitopes were readily identified by the direct comparison of the protein sequences of alleles that are structurally related that differ only by a few amino acids and have distinct serotypes. The evaluation of the reactivity of mAbs with different alleles allowed for the identification of residues possibly defining HLA class I epitopes to be mapped to residues shared by different alleles 10–14,30–35 . Critical residues defining serotypes or determining epitopes of HLA class II products were identified by similar approaches for HLA‐DR, 36–43 DQ 43–45 and DP 46–48 .…”

Section: Introductionmentioning

confidence: 99%

A new strategy for systematically classifying HLA alleles into serological specificities

Osoegawa

Marsh

Holdsworth

et al. 2022

HLA

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HLA serological specificities were defined by the reactivity of HLA molecules with sets of sera and monoclonal antibodies. Many recently identified alleles defined by molecular typing lack their serotype assignment. We surveyed the literature describing the correlation of the reactivity of serologic reagents with AA residues. 20 ‐ 25 AA residues determining epitopes (DEP) that correlated with 82 WHO serologic specificities were identified for HLA class I loci. Thirteen DEP each located in the beta‐1 domains that correlated with 24 WHO serologic specificities were identified for HLA‐DRB1 and ‐DQB1 loci. The designation of possible HLA‐DPB1, ‐DQA1, ‐DPA1, and additional serological specificities that result from epitopes defined by residues located at both ‐DQA1 and ‐DQB1 subunits were also examined. HATS software was developed for automated serotype assignments to HLA alleles in one of the three hierarchical matching criteria: (1) all DEP (FULL); (2) selected DEP specific to each serological specificity (SEROTYPE); (3) one AA mismatch with one or more SEROTYPES (INCOMPLETE). Results were validated by evaluating the alleles whose serotypes do not correspond to the first field of the allele name listed in the HLA dictionary. Additional 85 and 21 DEP patterns that do not correspond to any WHO serologic specificities for common HLA class I and DRB1 alleles were identified, respectively. A comprehensive antibody identification panel would allow for accurate unacceptable antigen listing and compatibility predictions in solid organ transplantation. We propose that antibody‐screening panels should include all serologic specificities identified in this study.

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Primary structure shows HLA‐B59 to be a hybrid of HLA‐B55 and HLA‐B51, and not a subtype of HLA‐B8

Abstract: Primary structure shows HLA-B59 to be a hybrid of HLA-B55 and HLA-B51, and not a subtype Tissue

Cited by 14 publications

References 15 publications

The nature of introns 4–6 suggests reduced lineage specificity in HLA-B alleles

The nature of introns 4–6 suggests reduced lineage specificity in HLA-B alleles

Low‐resolution DNA typing for HLA‐B using sequence‐specific primers in allele‐ or group‐specific ARMS/PCR

A new strategy for systematically classifying HLA alleles into serological specificities

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