The auxin‐binding protein Nt‐ERabp1 alone activates an auxin‐like transduction pathway

Leblanc, Nathalie; Perrot‐Rechenmann, Catherine; Barbier‐Brygoo, Hélène

doi:10.1016/s0014-5793(99)00398-1

Cited by 55 publications

(34 citation statements)

References 37 publications

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“…Various lines of evidence, mainly relying on electrophysiological experiments and use of a whole series of ABP1 molecular tools (specific monoclonal and polyclonal antibodies, carboxy-terminal synthetic peptides, recombinant wild-type and mutated proteins) support the hypothesis that ABP1 is involved in the initial perception of auxin at the outer face of the plasma membrane and induces the activation of H þ ATPase and K þ inward channels (Rück et al 1993;Thiel et al 1993;Barbier-Brygoo et al 1996;Fellner et al 1996;Leblanc et al 1999a;Leblanc et al 1999b;David et al 2001) (Fig. 3).…”

Section: Abp1 and Cell Expansionmentioning

confidence: 92%

Cellular Responses to Auxin: Division versus Expansion

Perrot‐Rechenmann¹

2010

Cold Spring Harbor Perspectives in Biology

462

339

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The phytohormone auxin is a major regulator of plant growth and development. Many aspects of these processes depend on the multiple controls exerted by auxin on cell division and cell expansion. The detailed mechanisms by which auxin controls these essential cellular responses are still poorly understood, despite recent progress in the identification of auxin receptors and components of auxin signaling pathways. The purpose of this review is to provide an overview of the present knowledge of the molecular mechanisms involved in the auxin control of cell division and cell expansion. In both cases, the involvement of at least two signaling pathways and of multiple targets of auxin action reflects the complexity of the subtle regulation of auxin-mediated cellular responses. In addition, it offers the necessary flexibility for generating differential responses within a given cell depending on its developmental context.

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Section: Abp1 and Cell Expansionmentioning

confidence: 92%

Cellular Responses to Auxin: Division versus Expansion

Perrot‐Rechenmann¹

2010

Cold Spring Harbor Perspectives in Biology

462

339

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“…After SDS-PAGE electrophoresis and transfer onto Hybond-C extra nitrocellulose membrane (GE Heathcare Europe), loading and protein transfer were controlled by transient red Ponceau staining. Protein gel blot analysis was performed as described previously (Leblanc et al, 1999a), using antipeptide rabbit polyclonal E-tag antibodies for detection of scFv12 and the mAb34 mouse monoclonal antibody for ABP1.…”

Section: Protein Analysismentioning

confidence: 99%

Conditional Repression of AUXIN BINDING PROTEIN1 Reveals That It Coordinates Cell Division and Cell Expansion during Postembryonic Shoot Development inArabidopsisand Tobacco

et al. 2008

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AUXIN BINDING PROTEIN1 (ABP1) has long been characterized as a potentially important mediator of auxin action in plants. Analysis of the functional requirement for ABP1 during development was hampered because of embryo lethality of the null mutant in Arabidopsis thaliana. Here, we used conditional repression of ABP1 to investigate its function during vegetative shoot development. Using an inducible cellular immunization approach and an inducible antisense construct, we showed that decreased ABP1 activity leads to a severe retardation of leaf growth involving an alteration in cell division frequency, an altered pattern of endocycle induction, a decrease in cell expansion, and a change in expression of early auxin responsive genes. In addition, local repression of ABP1 activity in the shoot apical meristem revealed an additional role for ABP1 in cell plate formation and cell shape. Moreover, cells at the site of presumptive leaf initiation were more sensitive to ABP1 repression than other regions of the meristem. This spatial context-dependent response of the meristem to ABP1 inactivation and the other data presented here are consistent with a model in which ABP1 acts as a coordinator of cell division and expansion, with local auxin levels influencing ABP1 effectiveness.

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“…Initially identified by its capacity to bind the phytohormone auxin, ABP1 was first shown to affect plasma membrane hyperpolarization via the modulation of ion fluxes across the membrane (Thiel et al, 1993;Barbier-Brygoo et al, 1996;Leblanc et al, 1999aLeblanc et al, , 1999b. These rapid ionic changes indicate a possible involvement of ABP1 in the control of cell expansion, at least in shoot tissues, thus providing preliminary molecular evidence supporting the acid growth theory.…”

Section: Introductionmentioning

confidence: 95%

AUXIN BINDING PROTEIN1 Links Cell Wall Remodeling, Auxin Signaling, and Cell Expansion in Arabidopsis

et al. 2014

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Cell expansion is an increase in cell size and thus plays an essential role in plant growth and development. Phytohormones and the primary plant cell wall play major roles in the complex process of cell expansion. In shoot tissues, cell expansion requires the auxin receptor AUXIN BINDING PROTEIN1 (ABP1), but the mechanism by which ABP1 affects expansion remains unknown. We analyzed the effect of functional inactivation of ABP1 on transcriptomic changes in dark-grown hypocotyls and investigated the consequences of gene expression on cell wall composition and cell expansion. Molecular and genetic evidence indicates that ABP1 affects the expression of a broad range of cell wall-related genes, especially cell wall remodeling genes, mainly via an SCF TIR/AFB -dependent pathway. ABP1 also functions in the modulation of hemicellulose xyloglucan structure. Furthermore, fucosidase-mediated defucosylation of xyloglucan, but not biosynthesis of nonfucosylated xyloglucan, rescued dark-grown hypocotyl lengthening of ABP1 knockdown seedlings. In muro remodeling of xyloglucan side chains via an ABP1-dependent pathway appears to be of critical importance for temporal and spatial control of cell expansion. INTRODUCTIONThe essential protein AUXIN BINDING PROTEIN1 (ABP1) functions in the control of growth and development throughout plant life. Initially identified by its capacity to bind the phytohormone auxin, ABP1 was first shown to affect plasma membrane hyperpolarization via the modulation of ion fluxes across the membrane (Thiel et al., 1993;Barbier-Brygoo et al., 1996;Leblanc et al., 1999aLeblanc et al., , 1999b. These rapid ionic changes indicate a possible involvement of ABP1 in the control of cell expansion, at least in shoot tissues, thus providing preliminary molecular evidence supporting the acid growth theory. This theory states that auxin promotes the excretion of protons at the apoplast resulting in cell wall loosening and increased growth rate (Rayle and Cleland, 1992). Binding of auxin to ABP1 and increased amount of ABP1 at the plasma membrane promote protoplast swelling and enhance expansion of leaf cells (Jones et al., 1998;Steffens et al., 2001;Christian et al., 2006). Conversely, the functional inactivation of ABP1 severely impairs cell expansion in shoot tissues irrespective of their DNA content (Braun et al., 2008;Xu et al., 2010) but does not affect root cell elongation (Tromas et al., 2009). The effect of ABP1 on cell expansion varies in a cell-or tissue-dependent manner. Recent data indicate that ABP1 acts both constitutively and in response to auxin (Robert et al., 2010;Tromas et al., 2013). The mechanism by which ABP1 controls cell expansion remains poorly understood. In shoot tissues, it remains unclear whether the contribution of ABP1 to cell expansion relies solely on nongenomic responses or acts also via the regulation of gene expression. ABP1 was reported to affect expression of various genes in response to auxin, but little is known on the broader effects of ABP1 on gene expression (Braun et al.,...

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The auxin‐binding protein Nt‐ERabp1 alone activates an auxin‐like transduction pathway

Cited by 55 publications

References 37 publications

Cellular Responses to Auxin: Division versus Expansion

Cellular Responses to Auxin: Division versus Expansion

Conditional Repression of AUXIN BINDING PROTEIN1 Reveals That It Coordinates Cell Division and Cell Expansion during Postembryonic Shoot Development inArabidopsisand Tobacco

AUXIN BINDING PROTEIN1 Links Cell Wall Remodeling, Auxin Signaling, and Cell Expansion in Arabidopsis

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