The autonomous parvovirus MVM encodes two nonstructural proteins in addition to its capsid polypeptides

Cotmore, Susan F.; Sturzenbecker, Laurie J.; Tattersall, Peter

doi:10.1016/0042-6822(83)90172-1

Cited by 104 publications

(107 citation statements)

References 24 publications

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“…3). ORF1 probably codes for non-structural protein(s) while ORF2 codes for the three known capsid proteins (30). 75% of the nucleotide differences found in these two ORFs occur in the third position of the codon, 13% in the second and 12% in the first position ( Table 1).…”

Section: Introductionmentioning

confidence: 99%

DNA sequence comparison between two tissue-specific variants of the autonomous parvovirus, minute virus of mice

Sahli¹,

McMaster

Hirt³

1985

Nucl Acids Res

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We have determined the complete nucleotide sequence of the DNA of the immunosuppressive variant of the parvovirus minute virus of mice (MVMi) and compared it to the published sequence (12) of the fibroblast-specific strain (MVMp). We have found 175 differences between the two viruses, most of which affect single nucleotides. Despite these differences, the genomic organization of MVMp and MVMi is identical. There are 29 amino-acid changes between the putative viral gene products of MVMi and MVMp, 16 of which are conservative. We discuss the possibility that the differential tissuespecificity of the two variants is linked to differences within the nontranscribed region near the 5' end of the viral genomes.

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Section: Introductionmentioning

confidence: 99%

DNA sequence comparison between two tissue-specific variants of the autonomous parvovirus, minute virus of mice

Sahli¹,

McMaster

Hirt³

1985

Nucl Acids Res

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“…It appears from our results that EA cells, which are permissive to MVM infection (12,36), contain sufficient activities of elongation factors which may act to prevent transcription attenuation. In contrast, WCE from MVM-infected EA cells does not prevent the elongation block at the MVM attenuation site.…”

Section: Resultsmentioning

confidence: 91%

“…MVM has a genome containing linear single-stranded DNA (5,149 nucleotides [nt]). It is an autonomous virus but is dependent for replication upon cellular functions that are expressed during S phase (10,12,13,36). Transcription initiation on the double-stranded replicative form catalyzed by the host RNA polymerase II has been shown to map to two MVM promoters: P4, located at nt 201 + 5; and P38, located at nt 2005 ± 4 (3,20,25).…”

mentioning

confidence: 99%

The block to transcription elongation at the minute virus of mice attenuator is regulated by cellular elongation factors.

Krauskopf¹,

Bengal²,

Aloni³

1991

Mol. Cell. Biol.

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We have previously reported that both in vivo and in vitro, RNA polymerase H pauses or prematurely terminates transcription at a specific attenuation site located 142 to 147 nucleotides downstream from the P4 promoter of minute virus of mice (MVM). In this report, we show that an in vitro block to transcription elongation in HeLa whole-cell extract occurs at elevated KCI concentrations (0.2 to 1.5 M) but not at the standard KCI concentration (50 mM). Briefly initiated transcription complexes, devoid of dissociated elongation factors by passage through a Sephacryl S-1000 column at 0.3 M KCI, were allowed to elongate the briefly initiated nascent RNA, and a block to transcription elongation at the attenuation site was observed independently of the KCl concentration at the time of elongation. Moreover, the block to elongation was overcome by the addition, during elongation, to the column of purified complexes of whole-cell extract from EA cells but not from MVM-infected EA cells or HeLa cells. The general transcription factors IIF and UX were also shown to alleviate this block to transcription elongation. On the basis of these results, we suggest that the block to elongation at the MVM attenuation site observed late in MVM infection results, at least in part, from the inactivation of the general transcription elongation factors.Recently, it has been shown that eukaryotic RNA polymerase II, like the prokaryotic polymerase, can pause or terminate both in vivo and in vitro transcription within viral (1,3,16,17,19,24,28,29,32,38) and cellular (4,8,9,15,18) genes and thus modulate downstream gene expression. This mechanism is termed attenuation (39). To date, however, little is known about cis and trans elements involved in the attenuation mechanism in eukaryotes. We have shown that at least in some viral systems, RNA polymerase II can respond to a stem-loop structure in the RNA followed by polyuridines as a signal for the elongation block in vitro (1,3,16,19,28,29,32). In other systems, different sequences constitute the attenuation signal (2,9,31,34,40).One of the model systems in our attenuation studies has been the parvovirus minute virus of mice (MVM). MVM has a genome containing linear single-stranded DNA (5,149 nucleotides [nt]). It is an autonomous virus but is dependent for replication upon cellular functions that are expressed during S phase (10,12,13,36). Transcription initiation on the double-stranded replicative form catalyzed by the host RNA polymerase II has been shown to map to two MVM promoters: P4, located at nt 201 + 5; and P38, located at nt 2005 ± 4 (3, 20, 25).We have previously shown that in vivo and in vitro, RNA polymerase II attenuates transcription at a specific location 142 to 147 nt downstream from the P4 promoter (3, 28). The attenuator RNA was found and mapped in vivo in A9 cells late after infection in both the nuclear and cytoplasmic fractions. Moreover, through the use of in vitro systems, we have shown that the attenuator RNA is a result of pausing or termination and not of processing...

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“…We also designated the fragment which contains 5' terminal of vDNA as 5' terminal fragment and the fragment containing 3' terminal of vDNA as 3' terminal fragment. RF DNA with Protein It has been reported in a number of different autonomous parvoviruses that the terminal protein binds to the RF DNA at the ends (9,22,27). We examined whether the protein attaching to MEV RF DNA was also bound at the ends.…”

Section: Methodsmentioning

confidence: 99%

“…Among the members of autonomous parvoviruses , minute virus of mice (MVM), H-l virus, and Kilham-rat virus have been well characterized on the synthesis of viral macromolecules involved in virus replication and structural features of the virus DNA (2)(3)(4)9). The virion DNA (vDNA) possesses at both termini unique structures of palindromes which are non-identical sequences at the 5' and 3' terminus (1)(2)(3)(4)23).…”

mentioning

confidence: 99%

Characterization of Replicative Form DNA of the Autonomous Parvovirus Mink Enteritis Virus

Shinagawa

Nomura

Kariatumari

et al. 1989

Microbiology and Immunology

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Characterization of replicative form (RF) DNA of mink enteritis virus (MEV) was carried out. Most of the RF DNA were bound to terminal protein but some were free from the protein. The protein-free RF DNA increased about 7 times from 30 to 50 hr post-infection, while the DNA with protein increased less . The molecules of the replicative intermediate which were partially single-stranded DNA and bound to terminal protein were present. Two terminal conformations , "extended" and "turnaround," were observed in both ends of both terminal proteinbound and protein-free RF DNA. The 5' end labeling revealed that 5' ends of protein-free RF DNA were not blocked to phosphorylation by an amino acid or an oligopeptide which attaches to 5' ends of proteolytically deproteinized RF DNA . Restriction analysis of incomplete RF DNA which was partially double-stranded DNA showed that extended conformation was dominant in such incomplete RF molecules.

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The autonomous parvovirus MVM encodes two nonstructural proteins in addition to its capsid polypeptides

Cited by 104 publications

References 24 publications

DNA sequence comparison between two tissue-specific variants of the autonomous parvovirus, minute virus of mice

DNA sequence comparison between two tissue-specific variants of the autonomous parvovirus, minute virus of mice

The block to transcription elongation at the minute virus of mice attenuator is regulated by cellular elongation factors.

Characterization of Replicative Form DNA of the Autonomous Parvovirus Mink Enteritis Virus

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