DNA sequence comparison between two tissue-specific variants of the autonomous parvovirus, minute virus of mice

Sahli, Roland; McMaster, Gary; Hirt, Bernhard

doi:10.1093/nar/13.10.3617

Cited by 89 publications

(75 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plasmid DNA was prepared for agarose gel electrophoresis and DNA sequencing by the alkaline lysis procedure of Birnboim and Doly (2) as modified by Sahli et al (19). Plasmid DNA was then run on 0.8% agarose gels to distinguish between plasmids carrying deletions or insertions and those which migrated with a mobility indistinguishable from that of the wild type.…”

Section: Methodsmentioning

confidence: 99%

Determination of the spectrum of mutations induced by defined-wavelength solar UVB (313-nm) radiation in mammalian cells by use of a shuttle vector.

Keyse¹,

Amaudruz²,

Tyrrell³

1988

Mol. Cell. Biol.

View full text Add to dashboard Cite

Mutations induced by UVB (313-nm) radiation, a wavelength in the region of peak effectiveness for sunlight-induced skin cancer in humans, have been analyzed at the sequence level in simian ceUs by using a plasmid shuttle vector (pZ189). We find that significant differences exist between the types of mutations induced by this solar wavelength and those induced by nonsolar UVC (254-nm) radiation. Compared with 254-nm radiation, 313-nm radiation induces more deletions and insertions in the region sequenced. In addition, although the types of base substitutions induced by the two wavelengths are broadly similar (in both cases, the majority of changes occur at G-C base pairs and the G-C to A-T transition is predominant), an analysis of the distribution of these base changes within the supF gene following irradiation at 313 nm reveals additional hot spots for mutation not seen after irradiation at 254 nm. These hot spots are shown to arise predominantly at sites of mutations involving multiple base changes, a class of mutations which arises more frequently at the longer solar wavelength. Lastly, we observe that most of the sites at which mutational hot spots arise after both UVC and UVB irradiation of the shuttle vector are also sites at which mutations arise spontaneously. Thus, a common mechanism may be involved in determining the site specificity of mutations, in which the DNA structure may be a more important determinant than the positions of DNA photoproducts.

show abstract

Section: Methodsmentioning

confidence: 99%

Determination of the spectrum of mutations induced by defined-wavelength solar UVB (313-nm) radiation in mammalian cells by use of a shuttle vector.

Keyse¹,

Amaudruz²,

Tyrrell³

1988

Mol. Cell. Biol.

View full text Add to dashboard Cite

show abstract

“…White and light blue (mutant) colonies were picked, restreaked on the same selective medium for confirmation of phenotype and stored as stabs in soft agar containing ampicillin until analysis. Plasmid DNA was prepared for further analysis by a modified alkaline lysis 'mini prep' method (24). Plasmids were then analysed by agarose gel electrophoresis to distinguish between those carrying large deletions or insertions and those that co-migrated with wild-type plasmid and probably contained point mutations.…”

Section: Mutant Selection and Characterizationmentioning

confidence: 99%

Mutagenesis by hydrogen peroxide treatment of mammalian cells: a molecular analysis

Moraes¹,

Keyse²,

Tyrrell³

1990

Carcinogenesis

113

View full text Add to dashboard Cite

Hydrogen peroxide is an oxidizing agent which can be generated intracellularly either during normal metabolism or by treatment with external agents including solar UV radiation. Simian cells (CV-1) transfected with the SV40-based shuttle vector plasmid pZ189 have been treated with H 2 O 2 and then incubated to allow repair and replication of the plasmid. The frequency of mutations at the supF locus of the recovered plasmid increases by a factor of up to four over the spontaneous value. The nucleotide changes associated with 100 spontaneous and 100 H 2 O 2 -induced mutants have been determined directly by sequencing a 150 bp fragment that includes the entire supF tRNA coding region. Deletions were observed in -45% of both the spontaneous and induced mutants, whereas single or multiple base changes arose in 68 and 57% of the induced and spontaneous mutants respectively. The spectrum of induced mutations is characterized by (i) the occurrence of deletions associated with base changes (16% of all mutants analysed) and (ii) small deletions of 3 bp and less (51% of all deletion mutants sequenced). Sixty-five per cent (15 out of 23) of all small deletions (spontaneous and induced) are associated with runs of between two and five identical bases and eight of them arise at a mutational 'hotspot' region of five cytosines between bp 172 and 176. The majority (19 out of 30) of completely sequenced deletions observed in the spontaneous spectrum contain either (i) small (2-10 bp) direct repeat sequences that lie immediately outside one deletion terminus and immediately inside the second deletion terminus or (ii) small (2-3 bp) inverted repeat sequences lying immediately inside the two deletion termini. Most deletions that we have observed are therefore likely to arise as a consequence of specific aspects of DNA structure. IntroductionOxygen radicals appear to be involved in both the ageing process and in the initiation and progression of many human diseases, including cancer. A knowledge of the nature of mutations arising in DNA as a result of oxygen radical attack should provide important clues to understanding the molecular events that underlie these pathological changes. The characterization of the genotoxic action of the oxidizing agent, hydrogen peroxide, is particularly relevant in this respect for several reasons. In addition

show abstract

“…Although MVMi contains a 3 splice site that performs poorly in fibroblasts, MVMi generated at least as much R2 and NS2 in murine lymphocytes as did MVMp in fibroblasts. Therefore, it appears that MVMp has acquired a mutation that improves the excision of the large intron, as it adapted to fibroblasts to accommodate the need for NS2 for replication in these cells, and that the ratio of NS1 to NS2 may play a larger role in the host range of MVM than previously appreciated.Two strains of minute virus of mice, fibrotropic strain MVMp and lymphotropic strain MVMi, are reciprocally restricted for growth in murine cells (2,10,15,23,25,26). MVMp productively infects murine fibroblasts but not murine lymphocytes, while MVMi does the opposite.…”

mentioning

confidence: 99%

“…Two strains of minute virus of mice, fibrotropic strain MVMp and lymphotropic strain MVMi, are reciprocally restricted for growth in murine cells (2,10,15,23,25,26). MVMp productively infects murine fibroblasts but not murine lymphocytes, while MVMi does the opposite.…”

mentioning

confidence: 99%

Replication of Minute Virus of Mice DNA Is Critically Dependent on Accumulated Levels of NS2

Choi

Newman

Burger

et al. 2005

J Virol

View full text Add to dashboard Cite

Following transfection of murine fibroblasts, the lymphotropic strain of minute virus of mice (MVMi) does not efficiently produce progeny single-strand DNA (ssDNA). However, changing a single nucleotide in the MVMi 3 splice site to that found in the fibrotropic strain MVMp enabled full DNA replication and production of ssDNA. This change enhanced excision of the large intron and the production of NS2, likely by improving interaction, in fibroblasts with the branch point-binding U2 snRNA. One function of NS2 involves interaction with the nuclear export protein Crm1. The defect in production of MVMi ssDNA in fibroblasts can also be overcome by introducing a mutation in MVMi NS2 that enhances its interaction with Crm1. Although MVMi contains a 3 splice site that performs poorly in fibroblasts, MVMi generated at least as much R2 and NS2 in murine lymphocytes as did MVMp in fibroblasts. Therefore, it appears that MVMp has acquired a mutation that improves the excision of the large intron, as it adapted to fibroblasts to accommodate the need for NS2 for replication in these cells, and that the ratio of NS1 to NS2 may play a larger role in the host range of MVM than previously appreciated.Two strains of minute virus of mice, fibrotropic strain MVMp and lymphotropic strain MVMi, are reciprocally restricted for growth in murine cells (2,10,15,23,25,26). MVMp productively infects murine fibroblasts but not murine lymphocytes, while MVMi does the opposite. The major viral determinant of this host range resides within the capsid. The primary block to restrictive infections is intracellular, following binding to cells but before deposition of viral DNA in the nucleus (1,3,4,11,12,20).Characterization of MVMp and MVMi has also led to the identification of another determinant that affects viral replication in a host cell-dependent manner. Analysis of MVMi virus selected for growth on fibroblasts and analysis of the replication of engineered MVMi and MVMp chimeras on murine lymphocytes have demonstrated that a region encompassing the large-intron 3Ј splice site and P38 TATA box also plays a role in the efficiency of viral DNA replication in these two cell types (7, 12). The role that this region plays in replication has not been well characterized, but in both cases, differences in the ratio of mRNA R1 to R2, which encode NS1 and NS2, respectively, have been observed (4, 7, 11). The importance of the NS region in cell type-specific replication has also been noted for cells of neuronal origin (22).Previous studies in our lab have shown that even minor alterations of the MVM large-intron 3Ј splice site can have significant effects on the steady-state ratio of R1 to R2 and, hence, on the ratio of the viral nonstructural proteins NS1 and NS2 (19,28). Differences between MVMi and MVMp in this area have been previously noted, and ways in which these differences might affect RNA processing in the two strains have been discussed previously (19). Recently, two reports have In this study we have shown that, following transfection of mur...

show abstract

DNA sequence comparison between two tissue-specific variants of the autonomous parvovirus, minute virus of mice

Cited by 89 publications

References 30 publications

Determination of the spectrum of mutations induced by defined-wavelength solar UVB (313-nm) radiation in mammalian cells by use of a shuttle vector.

Determination of the spectrum of mutations induced by defined-wavelength solar UVB (313-nm) radiation in mammalian cells by use of a shuttle vector.

Mutagenesis by hydrogen peroxide treatment of mammalian cells: a molecular analysis

Replication of Minute Virus of Mice DNA Is Critically Dependent on Accumulated Levels of NS2

Contact Info

Product

Resources

About