The atypical protein kinase RIOK3 contributes to glioma cell proliferation/survival, migration/invasion and the AKT/mTOR signaling pathway

Zhang, Tong; Ji, Daofei; Wang, Peng; Dong, Liang; Jin, Ling; Shi, Hengliang; Liu, Xuejiao; Meng, Qingming; Gao, Shangfeng

doi:10.1016/j.canlet.2017.12.010

Cited by 46 publications

(46 citation statements)

References 36 publications

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“…For quantitative Real‐Time PCR (qRT‐PCR) analysis, we stored fresh specimens at −135°C immediately after surgical resection; the clinicopathological data of these subjects were given in Table . For immunohistochemical analysis, the specimens were fixed in 10% buffered formalin and embedded in paraffin for sectioning; the clinicopathological information of these subjects was available in the Table of our previously published paper 9 . All glioma specimens had a confirmed pathological diagnosis and were classified according to the criteria of the World Health Organization (WHO).…”

Section: Methodsmentioning

confidence: 99%

“…To detect miR‐4744, we employed a cDNA reverse transcription kit (Takara, Dalian, China) and a pair of miR‐4744‐specific reverse transcription primer (Biomics Biotech, Nantong, China) to perform reverse transcription, and then used a SYBR Green PCR Master kit (Takara) and a miR‐4744 qRT‐PCR Detection Primer Set (Biomics Biotech) to perform qRT‐PCR. To detect U6 snRNA and other genes, reverse transcription and qRT‐PCR were performed using the similar protocol as described elsewhere 9 . The forward and reverse primers were shown in Table .…”

Section: Methodsmentioning

confidence: 99%

“…Total protein was extracted from tissues or cells, and a BCA Protein Assay Kit (Beyotime, Haimen, China) was used to determine protein concentration. Equal amounts of total protein were loaded for Western blot analysis as described in our recent paper 9 . The primary antibodies used were RIOK2 (1:500, Novus Biologicals), N‐cadherin (1:1000, Abcam), β‐catenin (1:2000, Cell Signaling Technology), Twist1 (1:500, Santa Cruz Bio), fibronectin (1:1000, BD), ZEB‐1 (1:500, Proteintech) and β‐actin (1:1500, Santa Cruz Bio).…”

Section: Methodsmentioning

confidence: 99%

“…RIOK2 acts as the substrate of PLK1 and is required for the proper mitotic progression in Hela cells 8 . In addition, our recent work has found that down‐regulation of RIOK3 causes G1 arrest, whereas overexpression of RIOK3 accelerates cell cycle progression in glioma cells 9 . Overall, RIO kinases play a key role in both ribosome synthesis and cell cycle progression, indicating their potential roles in tumour cell growth and expansion.…”

Section: Introductionmentioning

confidence: 98%

“…Knockdown of RIOK1 inhibits the proliferation, migration and invasion in breast, lung and colon cancer cells 10 . Stable overexpression of RIOK3 promotes proliferation, invasion and migration of glioma cells, while silencing of RIOK3 inhibits proliferation, migration and invasion, and induces apoptosis in glioma cells 9 . In addition, RIOK2 and RIOK1 are highly expressed in glioblastoma cells, overexpression of RIOK1/2 promotes cell proliferation, and down‐regulation of RIOK1/2 causes apoptosis and increased sensitivity to chemotherapy 11 .…”

Section: Introductionmentioning

confidence: 99%

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RIOK2 is negatively regulated by miR‐4744 and promotes glioma cell migration/invasion through epithelial‐mesenchymal transition

Song

Cheng

Jin

et al. 2020

J Cellular Molecular Medi

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RIOK2 is a member of RIO (right open reading frame) kinase family. Recent studies have revealed the involvement of RIO kinases in glioma cell growth and expansion. However, the role and mechanism of RIOK2 in glioma cell migration and invasion remain unclear. Wound healing assay, Transwell assay and real-time quantitative PCR (qRT-PCR) detection of matrix metalloproteinases (MMPs) were used to evaluate the migration/invasion of glioma cells. Western blot and qRT-PCR were employed to measure the expression of epithelial-mesenchymal transition (EMT) markers. Dual luciferase reporter assay was performed to determine the binding between RIOK2 and miR-4744. In addition, RIOK2 and miR-4744 levels were quantified by qRT-PCR and/ or immunohistochemistry in glioma tissues. Transfection of RIOK2 siRNAs significantly inhibited glioma cell migration and invasion and down-regulated the expression of MMPs (MMP2 and MMP9) and mesenchymal markers (N-cadherin, β-catenin, Twist1, fibronectin, ZEB-1) in glioma cells. Overexpression of RIOK2 showed the opposite effects. MiR-4744 directly bound to the 3'-untranslated region of RIOK2 and negatively regulated the expression of RIOK2. Up-regulation of miR-4744 inhibited the migration and invasion of glioma cells. Overexpression of RIOK2 could reverse the effects of miR-4744 up-regulation on the migration, invasion and EMT process in glioma cells. Moreover, RIOK2 was high, while miR-4744 was low in glioma tissues, and a negative correlation was found between them. These results suggest that RIOK2 is post-transcriptionally targeted by miR-4744, the low miR-4744 and high RIOK2 levels in glioma may contribute to tumour cell infiltration through promoting the EMT. K E Y W O R D S fibronectin, glioma, miR-4744, N-cadherin, RIOK2, β-catenin | 4495 SONG et al. S U PP O RTI N G I N FO R M ATI O N Additional supporting information may be found online in the Supporting Information section. How to cite this article: Song Y, Li C, Jin L, et al. RIOK2 is negatively regulated by miR-4744 and promotes glioma cell migration/invasion through epithelial-mesenchymal transition.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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RIOK2 is negatively regulated by miR‐4744 and promotes glioma cell migration/invasion through epithelial‐mesenchymal transition

Song

Cheng

Jin

et al. 2020

J Cellular Molecular Medi

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Rosmarinic acid, the active component of Rubi Fructus, induces apoptosis of SGC-7901 and HepG2 cells through mitochondrial pathway and exerts anti-tumor effect

Chen

Liu

Shen

et al. 2023

Naunyn-Schmiedeberg's Arch Pharmacol

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Rosmarinic acid (RA) is a well-known phenolic acid widely present in over 160 species of herbal plants and known to exhibit anti-tumor effects on breast, prostate, and colon cancers in vitro. However, its effect and mechanism in gastric cancer and liver cancer are unclear. Moreover, there is no RA report yet in the chemical constituents of Rubi Fructus (RF). In this study, RA was isolated from RF for the first time, and the effect and mechanism of RA on gastric and liver cancers were evaluated using SGC-7901 and HepG2 cells models. The cells were treated with different concentrations of RA (50, 75, and 100 μg/mL) for 48 h, and the effect of RA on cell proliferation was evaluated by the CCK-8 assay. The effect of RA on cell morphology and mobility was observed by inverted fluorescence microscopy, cell apoptosis and cell cycle were determined by flow cytometry, and the expression of apoptosis-related proteins cytochrome C, cleaved caspase-3, Bax, and Bcl-2 was detected by western blotting. The results revealed that, with an increase in the RA concentration, the cell viability, mobility, and Bcl-2 expression decreased, while the apoptosis rate, Bax, cytochrome C, and cleaved caspase-3 expression increased, and SGC-7901 and HepG2 cells could be induced to arrest their cell cycle in the G0/G1 and S phases, respectively. These results together indicate that RA can induce apoptosis of SGC-7901 and HepG2 cells through the mitochondrial pathway. Thus, this study supplements the material basis of the anti-tumor activity of RF and provides an insight into the potential mechanism of RA-inducing apoptosis of gastric cancer SGC-7901 cells and liver cancer HepG2 cells, thereby facilitating further developmental studies on and the utilization of the anti-tumor activity of RF.

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The Effect and Mechanism of LINC00663 on the Biological Behavior of Glioma

et al. 2021

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The atypical protein kinase RIOK3 contributes to glioma cell proliferation/survival, migration/invasion and the AKT/mTOR signaling pathway

Cited by 46 publications

References 36 publications

RIOK2 is negatively regulated by miR‐4744 and promotes glioma cell migration/invasion through epithelial‐mesenchymal transition

RIOK2 is negatively regulated by miR‐4744 and promotes glioma cell migration/invasion through epithelial‐mesenchymal transition

Rosmarinic acid, the active component of Rubi Fructus, induces apoptosis of SGC-7901 and HepG2 cells through mitochondrial pathway and exerts anti-tumor effect

The Effect and Mechanism of LINC00663 on the Biological Behavior of Glioma

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