The attenuated sopB mutant of Salmonella enterica serovar Typhimurium has the same tissue distribution and host chemokine response as the wild type in bovine Peyer’s patches

Reis, Bruno Pinheiro Wanderley; Zhang, Shuping; Tsolis, Renée M.; Bäumler, Andreas J.; Adams, L. Garry; Santos, Renato L.

doi:10.1016/j.vetmic.2003.09.019

Cited by 38 publications

(31 citation statements)

References 16 publications

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“…Upon translocation through the intestinal epithelium, bacteria are found mainly within mononuclear cells (i.e., macrophages or dendritic cells) or neutrophils in the lamina propria (25,27). Some of the cytokines released by mononuclear cells after their stimulation with bacteria may help to amplify inflammatory responses in tissue (29).…”

Section: Discussionmentioning

confidence: 99%

T Cells Help To Amplify Inflammatory Responses Induced bySalmonella entericaSerotype Typhimurium in the Intestinal Mucosa

et al. 2008

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Section: Discussionmentioning

confidence: 99%

T Cells Help To Amplify Inflammatory Responses Induced bySalmonella entericaSerotype Typhimurium in the Intestinal Mucosa

et al. 2008

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“…Serotype Typhimurium readily enters enterocytes and M cells during infection of bovine ligated ileal loops, an in vivo model for human gastroenteritis (29,95,101). Analysis of this process using human epithelial cell lines shows that Salmonella serotypes trigger entry by injecting proteins, termed effectors, into host cells using type III secretion system 1 (T3SS-1), encoded by Salmonella pathogenicity island 1 (SPI1) (31,32,70).…”

Section: Inflammatory Diarrhea: a Stereotypic Host Response To Microbmentioning

confidence: 99%

“…Subsequent to invasion, serotype Typhimurium traverses the epithelium to reach the lamina propria, where bacteria always have an intracellular location within phagocytes (neutrophils and mononuclear cells) (29,95,101). Intestinal epithelial cells can differentiate commensal bacteria present in the intestinal lumen from serotype Typhimurium organisms present in the lamina propria by expressing a pathogen recognition receptor, termed Toll-like receptor 5 (TLR5), exclusively on their basolateral surfaces (34).…”

Section: Inflammatory Diarrhea: a Stereotypic Host Response To Microbmentioning

confidence: 99%

Capsule-Mediated Immune Evasion: a New Hypothesis Explaining Aspects of Typhoid Fever Pathogenesis

et al. 2006

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“…Initial inflammatory responses elicited by S. typhimurium in the intestine are important for the disease outcome, as they enable immunocompetent people to contain bacteria at the site of infection, thereby causing a localized gastroenteritis. The induction of this rapid inflammatory response can be studied by using S. typhimurium infection of bovine ligated ileal loops, where inflammation is initiated by bacterial invasion of the intestinal epithelium 7 , resulting in translocation into the lamina propria within 1 to 2 h after infection 8 . Within the lamina propria, S. typhimurium is observed within mononuclear phagocytes or neutrophils 9 .…”

mentioning

confidence: 99%

Simian immunodeficiency virus–induced mucosal interleukin-17 deficiency promotes Salmonella dissemination from the gut

Raffatellu

Santos

Verhoeven

et al. 2008

Nat Med

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Salmonella typhimurium causes a localized enteric infection in immunocompetent individuals, whereas HIV-infected individuals develop a life-threatening bacteremia. Here we show that simian immunodeficiency virus (SIV) infection results in depletion of T helper type 17 (T H 17) cells in the ileal mucosa of rhesus macaques, thereby impairing mucosal barrier functions to S. typhimurium dissemination. In SIV-negative macaques, the gene expression profile induced by S. typhimurium in ligated ileal loops was dominated by T H 17 responses, including the expression of interleukin-17 (IL-17) and IL-22. T H 17 cells were markedly depleted in SIV-infected rhesus macaques, resulting in blunted T H 17 responses to S. typhimurium infection and increased bacterial dissemination. IL-17 receptor-deficient mice showed increased systemic dissemination of S. typhimurium from the gut, suggesting that IL-17 deficiency causes defects in mucosal barrier function. We conclude that SIV infection impairs the IL-17 axis, an arm of the mucosal immune response preventing systemic microbial dissemination from the gastrointestinal tract. A.G. served as consultant for the presentation of NTS bacteremia in African subjects. J.K.K. served as collaborator on studies with Il17ra −/− mice and provided useful comments on the experimental design. S.D. designed and supervised the SIV infections of rhesus macaques, blood sample scheduling, macaque protocols, processing and cell isolations for flow cytometry and DNA microarray analyses. A.J.B. was responsible for the experimental design and supervision of mouse studies, ligated ileal loop experiments in rhesus macaques, macaque protocols and analysis of host responses to Salmonella infection. A.J.B. collected tissue during the ligated ileal loop surgery and was responsible for the final manuscript preparation. A.J.B. and S.D. provided financial support for the study and equally contributed to the experimental design, supervision and data interpretation. Although nontyphoidal Salmonella serotypes (NTS) are common agents causing acute foodborne disease worldwide, it is unusual to isolate them from the blood of adults, because in immunocompetent individuals these pathogens remain localized to the intestine and cause a self-limiting gastroenteritis 1 . However, in people with underlying immunosuppression, NTS may spread beyond the intestine and reach the bloodstream, a serious complication known as NTS bacteremia2. The rise in the number of people with AIDS in sub-Saharan Africa has led to a dramatic increase in the frequency of NTS bacteremia3. In marked contrast to the pre-AIDS era4, NTS is currently a leading cause of hospital admission of adults and among the most common bacterial blood isolates from hospitalized adults in sub-Saharan Africa5, the vast majority of whom are HIV positive 3 . NTS infection in HIV-positive African adults is associated with high acute mortality rates (47%) 6 . Although the occurrence of NTS bacteremia in HIV-positive people is well documented, there are no reports inv...

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The attenuated sopB mutant of Salmonella enterica serovar Typhimurium has the same tissue distribution and host chemokine response as the wild type in bovine Peyer’s patches

Cited by 38 publications

References 16 publications

T Cells Help To Amplify Inflammatory Responses Induced bySalmonella entericaSerotype Typhimurium in the Intestinal Mucosa

T Cells Help To Amplify Inflammatory Responses Induced bySalmonella entericaSerotype Typhimurium in the Intestinal Mucosa

Capsule-Mediated Immune Evasion: a New Hypothesis Explaining Aspects of Typhoid Fever Pathogenesis

Simian immunodeficiency virus–induced mucosal interleukin-17 deficiency promotes Salmonella dissemination from the gut

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