The AtGSTU7 gene influences glutathione-dependent seed germination under ABA and osmotic stress in Arabidopsis

Wu, Juan; Zhang, Nan; Liu, Ziguang; S, Liu; Liu, Chunxiao; Lin, Jian-Hui; yang, he; Li, Shuang; Yukawa, Yasushi

doi:10.1016/j.bbrc.2020.05.153

Cited by 21 publications

(22 citation statements)

References 21 publications

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“…Therefore, GSTs affect a range of redox-dependent cellular processes that involve hormone and stress responses including ROS-mediated ABA metabolism and signaling. Consistently, the gstu7 and gstu17 mutants of Arabidopsis have been reported to exhibit increased GSH and ABA levels and decreased H 2 O 2 levels, and the seeds of these mutants are found to be less sensitive to ABA during germination [47,48]. Furthermore, the gstu7 mutant shows reduction in the expression levels of genes encoding proteins that act as positive regulators of ABA signaling including SnRK, ABI3 and ABI5 [48].…”

Section: Glutathione Modulates Aba Signaling In the Regulation Of Seed Dormancy And Germinationmentioning

confidence: 75%

“…Previous studies have shown that enzymes which regulate the GSH pool have a significant effect on ABA signaling and the regulation of seed germination and dormancy. For example, genes encoding GPX and GST enzymes, which modulate cellular GSH levels, have been shown to play roles in regulating ABA signaling in arresting seed germination [46][47][48][49]. However, an increase in the levels of GSH-containing glutathionylated proteins/compounds such as GRX and GSNO have been shown to suppress ABA signaling during seed germination [50,51].…”

Section: Glutathione Modulates Aba Signaling In the Regulation Of Seed Dormancy And Germinationmentioning

confidence: 99%

“…Furthermore, exogenous application of ABA, for example in wheat, maize and soybean, leads to upregulation of genes involved in GSH synthesis and/or an increase in GSH level, resulting in an enhanced tolerance to drought [84,96,97]. Conversely, treatment with exogenous GSH or overexpression of the GSH biosynthesis gene GSH1 in Arabidopsis improves drought tolerance through altering the expression patterns of ABA metabolic and signaling genes, leading to upregulation of the downstream ABA responsive genes [12,47]. Despite these reports, the mechanisms underlying the interaction between GSH and ABA in regulating plant response to drought remain to be elucidated.…”

Section: Glutathione-mediated Aba Signaling In Drought Tolerancementioning

confidence: 99%

“…These results suggest the role of GSTU genes in mediating the ABA-dependent oxidative and drought stress tolerance. In contrast, the Arabidopsis GSTU17 has been shown to function as a negative regulator of drought-mediated signal transduction pathways; the gstu17 mutant exhibits increased GSH and ABA levels, reduced stomatal aperture and transpirational water loss rates, leading to enhanced tolerance to drought [47]. Given that GST, which is encoded by large gene families, has multiple functions [100], its role in ABA-mediated drought stress signaling might vary with plant species.…”

Section: Glutathione Peroxidase and Glutathione S-transferases As Regulators Of Gsh Pool And Drought-induced Aba Signalingmentioning

confidence: 99%

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Roles of Glutathione in Mediating Abscisic Acid Signaling and Its Regulation of Seed Dormancy and Drought Tolerance

Koramutla

Negi

Ayele

2021

Genes

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Plant growth and development and interactions with the environment are regulated by phytohormones and other signaling molecules. During their evolution, plants have developed strategies for efficient signal perception and for the activation of signal transduction cascades to maintain proper growth and development, in particular under adverse environmental conditions. Abscisic acid (ABA) is one of the phytohormones known to regulate plant developmental events and tolerance to environmental stresses. The role of ABA is mediated by both its accumulated level, which is regulated by its biosynthesis and catabolism, and signaling, all of which are influenced by complex regulatory mechanisms. Under stress conditions, plants employ enzymatic and non-enzymatic antioxidant strategies to scavenge excess reactive oxygen species (ROS) and mitigate the negative effects of oxidative stress. Glutathione (GSH) is one of the main antioxidant molecules playing a critical role in plant survival under stress conditions through the detoxification of excess ROS, maintaining cellular redox homeostasis and regulating protein functions. GSH has recently emerged as an important signaling molecule regulating ABA signal transduction and associated developmental events, and response to stressors. This review highlights the current knowledge on the interplay between ABA and GSH in regulating seed dormancy, germination, stomatal closure and tolerance to drought.

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Section: Glutathione Modulates Aba Signaling In the Regulation Of Seed Dormancy And Germinationmentioning

confidence: 75%

Section: Glutathione Modulates Aba Signaling In the Regulation Of Seed Dormancy And Germinationmentioning

confidence: 99%

Section: Glutathione-mediated Aba Signaling In Drought Tolerancementioning

confidence: 99%

Section: Glutathione Peroxidase and Glutathione S-transferases As Regulators Of Gsh Pool And Drought-induced Aba Signalingmentioning

confidence: 99%

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Roles of Glutathione in Mediating Abscisic Acid Signaling and Its Regulation of Seed Dormancy and Drought Tolerance

Koramutla

Negi

Ayele

2021

Genes

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“…nsLTP has been reported to be implicated in pollen tube tip growth and fertilization ( Chae et al, 2009 ), and GST protein was involved in the transport of pigment to vacuoles where it accumulates ( Momose et al, 2013 ). In Arabidopsis , GSTU7 has a role in seed germination mediated by GSH-ROS homeostasis and ABA signaling ( Wu et al, 2020 ). Thus, nsLTP and GSTU7 proteins play important roles in the development of saffron floral organs, such as the pistil and stamen, where pollen and most pigments are deposited.…”

Section: Resultsmentioning

confidence: 99%

Screening of Key Proteins Affecting Floral Initiation of Saffron Under Cold Stress Using iTRAQ-Based Proteomics

Chen

Gui-fen

Dong³

et al. 2021

Front. Plant Sci.

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BackgroundSaffron crocus (Crocus sativus) is an expensive and valuable species that presents preventive and curative effects. This study aimed to screen the key proteins affecting the floral initiation of saffron under cold stress and thus increasing yield by regulating the temperature.ResultsProtein expression profiles in flowering and non-flowering saffron buds were established using isobaric tags for relative or absolute quantitation (iTRAQ). A total of 5,624 proteins were identified, and 201 differentially abundant protein species (DAPs) were further obtained between the flowering and non-flowering groups. The most important functions of the upregulated DAPs were “sucrose metabolic process,” “lipid transport,” “glutathione metabolic process,” and “gene silencing by RNA.” Downregulated DAPs were significantly enriched in “starch biosynthetic process” and several oxidative stress response pathways. Three new flower-related proteins, CsFLK, CseIF4a, and CsHUA1, were identified in this study. The following eight key genes were validated by real-time qPCR in flowering and non-flowering top buds from five different growth phases: floral induction- and floral organ development-related genes CsFLK, CseIF4A, CsHUA1, and CsGSTU7; sucrose synthase activity-related genes CsSUS1 and CsSUS2; and starch synthase activity-related genes CsGBSS1 and CsPU1. These findings demonstrate the important roles played by sucrose/starch biosynthesis pathways in floral development at the mRNA level. During normal floral organ development, the sucrose contents in the top buds of saffron increased, and the starch contents decreased. In contrast, non-flowering buds showed significantly decreased sucrose contents under cold stress and no significant changes in starch contents compared with those in the dormancy stage.ConclusionIn this report, the protein profiles of saffron under cold stress and a normal environment were revealed for the first time by iTRAQ. A possible “reactive oxygen species–antioxidant system–starch/sugar interconversion flowering pathway” was established to explain the phenomenon that saffron does not bloom due to low temperature treatment.

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Roles of cytokinins in root growth and abiotic stress response of Arabidopsis thaliana

Liu

Wang

et al. 2021

Plant Growth Regul

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The AtGSTU7 gene influences glutathione-dependent seed germination under ABA and osmotic stress in Arabidopsis

Cited by 21 publications

References 21 publications

Roles of Glutathione in Mediating Abscisic Acid Signaling and Its Regulation of Seed Dormancy and Drought Tolerance

Roles of Glutathione in Mediating Abscisic Acid Signaling and Its Regulation of Seed Dormancy and Drought Tolerance

Screening of Key Proteins Affecting Floral Initiation of Saffron Under Cold Stress Using iTRAQ-Based Proteomics

Roles of cytokinins in root growth and abiotic stress response of Arabidopsis thaliana

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