The ataxias

Subramony, S. H.; Wood, Nicholas W.

doi:10.1017/cbo9781139087711.005

Cited by 1 publication

(1 citation statement)

References 92 publications

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“…Electrospun fibers have been increasingly recognized as a biomimetic platform for bone tissue engineering due to their versatility in mimicking extracellular matrix (ECM) [43][44][45][46] as well as for the benefits of their high surface-to-volume ratios for cell attachment, drug loading and mass transport. [47][48] In particular, coaxially electrospun nanofibers have recently emerged as promising matrices for controlled release of hydrophilic drugs for therapeutic purposes.…”

Section: Introductionmentioning

confidence: 99%

Electrospun Fiber Mesh for High-Resolution Measurements of Oxygen Tension in Cranial Bone Defect Repair

Schilling

Khatib

Plunkett

et al. 2019

ACS Appl. Mater. Interfaces

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Tissue oxygenation is one of the key determining factors in bone repair and bone tissue engineering. Adequate tissue oxygenation is essential for survival and differentiation of the boneforming cells and ultimately the success of bone tissue regeneration. Two-photon phosphorescence lifetime microscopy (2PLM) has been successfully applied in the past to image oxygen distributions in tissue with high spatial resolution. However, delivery of phosphorescent probes into avascular compartments, such as those formed during early bone defect healing, poses significant problems. Here we report a multifunctional oxygen-reporting fibrous matrix fabricated through encapsulation of a hydrophilic oxygen-sensitive, two-photon excitable phosphorescent probe PtP-C343 in the core of fibers during coaxial electrospinning. The oxygen-sensitive fibers support bone marrow stromal cell (BMSC) growth and differentiation, while at the same time enable real-time high-resolution probing of partial pressures of oxygen (pO 2 ) via 2PLM. The hydrophilicity of the probe further facilitates its gradual release into the nearby microenvironment, allowing fibers to act as a vehicle for probe delivery into the healing tissue. In conjunction with a cranial defect window chamber model, which permits simultaneous imaging of the bone and neovasculature in vivo via two-photon laser scanning microscopy (2PLSM), the oxygen reporting fibers provide a useful tool for minimally invasive, high-resolution, real-time 3D mapping of tissue oxygenation during bone defect healing, facilitating studies aimed at understanding the healing process and advancing design of tissue engineered constructs for enhanced bone repair and regeneration.

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