The Association of Peroxidase Activity and Resistance of Maize to Exserohilum turcicum

Abstract

Peroxidase activity in leaves of maize (Zea mays L.) differing in susceptibility to Exserohilum turcicum has been investigated in relation to their resistance to Northern Leaf Blight (NLB) caused by the fungal pathogen E. turcicum. In non‐inoculated plants, high peroxidase activity was detected in leaves of the resistant isolines B37HtN and B73HtN as compared with the susceptible isolines B37 and B73 and the sweet corn variety Jubilee. After inoculation with E. turcicum, peroxidase activity increased in both susceptible and resistant isolines B73 and B73HtN. However, marked enhancement of peroxidase activity was detected 6 days after inoculation and became remarkable in isoline B73HtN, although symptomes started to show up in both susceptible and resistant plants only 10 days after inoculation. Using polyacrylamide gel electrophoresis separations, different banding pattern of isoperoxidases was found in the susceptible plants as compared with the resistant ones. In non‐inoculated plants, three differential bands which appeared in the resistant isoline B37HtN, were absent in the susceptible Jubilee plants, and were as traces in the isoline B37. These bands first appeared in Jubilee and as clear bands in B37, only after inoculation with E. turcicum. The association of these isoperoxidases and resistance of maize to E. turcicum is discussed.

Effect of phytoplasma infection on the activity of peroxidase, β-1,3 glucanase and chitinase in corn plants

“…Higher peroxidase activity was suggested to interfere with H 2 O 2 concentration and other metabolites allowing higher phytoplasma activity in the tissues. On the other hand, these results are different from those shown for the pathosystem maize-Exserohilum turcicum, in which a rapid increase in peroxidase activity occurred in resistant hybrids resulting in later symptoms (27). In the pathosystem maize-phytoplasma used in the present study only quantitative alterations in peroxidase activity were observed.…”

The effects of cold-hardening and Microdochium nivale infection on oxidative stress and antioxidative protection of the two contrasting genotypes of winter triticale

“…A diversity of reactions catalysed by POXs as well as a large number of their genes suggests a possible functional specialization for each isoform [60]. The earlier results of studies carried out by Reuveni and co-workers [32,61] suggested that POX activity is a biochemical marker, which may or may not be part of the resistance mechanism, but can be used to predict resistance to disease. In Takashima et al [62] research, cationic peroxidase was related to basal resistance of Betula platyphylla var.…”

Oxidative Stress Defence Response of Carica papaya Challenged by Nitric Oxide, Papaya meleira virus and Saccharomyces cerevisiae

“…Peroxidase activity is increased in plants infected with fungus [20], and peroxidase levels in maize rose faster post infection in varieties of maize resistant to Exserohilum turcicum [19]. Increase in peroxidase activity is also a response to viral infection, and has been reported in tobacco [45], peaches and apricots [21] and beans [22].…”

“…Peroxidases, together with catalases, are the major H 2 O 2 degrading enzymes in plant cells [16], although the reaction of peroxidases with H 2 O 2 is a process which can lead to lipid peroxidation and thus membrane damage [17]. Several authors have found a relationship between peroxidase levels and resistance to fungal [18][19][20] or viral [21,22] pathogens.…”