The association of gastric leptin with oesophageal inflammation and metaplasia

François, Fritz; Roper, Jatin; Goodman, Adam; Pei, Zhiheng; Ghumman, Muhammad; Mourad, Michelle; Perez, Asalia Z. Olivares de; Perez-Perez, Guillermo I.; Tseng, Chieh; Blaser, M. J.

doi:10.1136/gut.2007.131672

Cited by 75 publications

(61 citation statements)

References 69 publications

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“…Patients with Barrett's esophagus have significantly higher fundic leptin levels compared with a normal esophagus. 23 High serum leptin is associated with Barrett's esophagus in men but not women. 24 In a case-control study of Barrett's esophagus compared with controls (50 matched pairs), Barrett's esophagus was inversely associated with the plasma adiponectin level, suggesting that adiponectin may be involved in the etiology of Barrett's esophagus.…”

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confidence: 99%

The Effect of Abdominal Visceral Fat, Circulating Inflammatory Cytokines, and Leptin Levels on Reflux Esophagitis

Nam

Choi

Ryu

et al. 2015

J Neurogastroenterol Motil

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Background/AimsAlthough adipocytes secrete inflammatory cytokines and adipokines, their role in reflux esophagitis is controversial. We investigated the association between visceral fat and inflammatory cytokines or adipokines in reflux esophagitis. MethodsAbdominal visceral fat and cytokines were measured in 66 individuals with reflux esophagitis and 66 age-and sex-matched controls. The mean values for visceral fat and cytokines were compared in cases and controls. Second, correlations between visceral fat and inflammatory cytokines were measured. Finally, multiple logistic regression models for odds ratios (ORs) and 95% confidence intervals (CIs) were used to estimate the effects of visceral fat and cytokines on reflux esophagitis. ResultsVisceral fat, leptin, interleukin (IL)-6, and IL-1 were higher in reflux esophagitis compared to controls. Visceral fat showed a strong positive correlation with IL-6 (r = 0.523, P < 0.001), IL-8 (r = 0.395, P < 0.001), and IL-1 (r = 0.557, P < 0.001), and a negative correlation with adiponectin (r = −0.466, P < 0.001). With adjusted analysis, visceral fat/100 (OR, 4.32; 95% CI, 2.18-8.58; P < 0.001) and leptin (OR, 1.36; 95% CI, 1.10-1.69; P = 0.005) independently increased the risk of reflux esophagitis, but the effects of other cytokines were abolished.

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confidence: 99%

The Effect of Abdominal Visceral Fat, Circulating Inflammatory Cytokines, and Leptin Levels on Reflux Esophagitis

Nam

Choi

Ryu

et al. 2015

J Neurogastroenterol Motil

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“…The persistence of leptin receptors in human oesophageal epithelium (Francois et al 2008) confirms the importance of leptin in oesophageal functions. Further studies into the localisation of leptin receptors in the sea bass oesophagus could confirm this role.…”

Section: Discussionmentioning

confidence: 62%

Immunolocalisation of leptin in the digestive system of juvenile European sea bass (Dicentrarchus labrax)

Gambardella

Ferrando

et al. 2010

Italian Journal of Zoology

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Leptin is a hormone that is mainly produced by adipocytes; it is connected with metabolism regulation, but knowledge about its effect on teleosts, particularly in juvenile specimens, is still scarce. This study is the first to investigate the presence of leptin immunoreactivity in juvenile European sea bass (Dicentrarchus labrax) and its distribution in the digestive system. Leptin immunoreactivity was detected by Western blot analysis, while its distribution was obtained using immunohistochemical methods with a human leptin-specific antibody. The homogenate of the body of a whole European sea bass contains a leptin-like protein with an estimated molecular weight of approximately 16 kDa. Leptin immunoreactivity was distributed along the digestive system, especially in the oesophageal mucous cells, neuroendocrine gastrointestinal cells and eosinophilic granular cells of the gastrointestinal lamina propria. This is the first report on leptin immunoreactivity in juvenile sea bass and our data can provide a good morphological basis for further investigation into food, metabolism and immunoresponse regulation in aquacultured fish.

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“…We studied the sera from 247 adults undergoing endoscopy at the VA New York Harbor Healthcare System. Patients were considered H. pylori positive if they showed positive results for at least two of the following assays: histology, culture, serology, and rapid urease testing (24). In brief, enzyme-linked immunosorbent assays (ELISAs) were conducted by fixing 10 ng of recombinant full-length JHP_0110 protein to each well, using carbonate buffer (pH 9.6).…”

Section: Methodsmentioning

confidence: 99%

Repeat-Associated Plasticity in the Helicobacter pylori RD Gene Family

et al. 2009

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The bacterium Helicobacter pylori is remarkable for its ability to persist in the human stomach for decades without provoking sterilizing immunity. Since repetitive DNA can facilitate adaptive genomic flexibility via increased recombination, insertion, and deletion, we searched the genomes of two H. pylori strains for nucleotide repeats. We discovered a family of genes with extensive repetitive DNA that we have termed the H. pylori RD gene family. Each gene of this family is composed of a conserved 3 region, a variable mid-region encoding 7 and 11 amino acid repeats, and a 5 region containing one of two possible alleles. Analysis of five complete genome sequences and PCR genotyping of 42 H. pylori strains revealed extensive variation between strains in the number, location, and arrangement of RD genes. Furthermore, examination of multiple strains isolated from a single subject's stomach revealed intrahost variation in repeat number and composition. Despite prior evidence that the protein products of this gene family are expressed at the bacterial cell surface, enzyme-linked immunosorbent assay and immunoblot studies revealed no consistent seroreactivity to a recombinant RD protein by H. pylori-positive hosts. The pattern of repeats uncovered in the RD gene family appears to reflect slipped-strand mispairing or domain duplication, allowing for redundancy and subsequent diversity in genotype and phenotype. This novel family of hypervariable genes with conserved, repetitive, and allelic domains may represent an important locus for understanding H. pylori persistence in its natural host.

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The association of gastric leptin with oesophageal inflammation and metaplasia

Abstract: Leptin receptor expression on oesophageal epithelial cells provides a pathway for leptin-mediated signal transduction. Variation in gastric leptin production could contribute to differential oesophageal healing and metaplasia progression.

Cited by 75 publications

References 69 publications

The Effect of Abdominal Visceral Fat, Circulating Inflammatory Cytokines, and Leptin Levels on Reflux Esophagitis

The Effect of Abdominal Visceral Fat, Circulating Inflammatory Cytokines, and Leptin Levels on Reflux Esophagitis

Immunolocalisation of leptin in the digestive system of juvenile European sea bass (Dicentrarchus labrax)

Repeat-Associated Plasticity in the Helicobacter pylori RD Gene Family

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