THE ASSOCIATION BETWEEN B-RAF V600E GENE MUTATION WITH AGE, GRADING, AND CLINICAL RESPONSE IN COLORECTAL CANCER AT THE CENTER GENERAL HOSPITAL (RSUP) PROF. Dr. I GOESTI NGOERAH GDE NGOERAH IN BALI

Abstract

Background: Colorectal cancer (CRC) is the most common type of cancer found in people over 50 years of age and is a disease associated with aging. CRC is the most preventable and curable cancer due to the pre-clinical phase until it becomes cancer between 10 -20 years. The B-RAF mutation plays an important role in the prognosis and treatment of CRC patients. The B-RAF V600E mutation is the most common B-RAF mutation. This study aims to analyze the relationship between mutations in the B-RAF V600E gene with age, grading, and clinical response in CRC patients in Bali. Method: We used FFPE samples that were confirmed as CRC tissues by the pathologist. Identification of BRAF V600E mutation was identified using polymerase chain reaction (PCR) and direct sequencing. Data on age, grade, and clinical response were recorded from the patient's medical record. Data was analized using Fisher-exact test. Results: The B-RAF V600E mutation samples were identified in 3 out of 74 samples (4.1%). The B-RAF V600E mutation was found in 3/3 of the samples (100%), which aged over 50 years old. All those mutated cases (100%) were low grade. Positive vs. negative clinical response was shown at 2/3 (66.7%) samples vs. 1/3 (33.3%) sample. No significant correlation was found between B-RAF V600E gene mutation and age (p=0.643), grading (p=0.808), and clinical response (p=0.358). Conclusion:We have identified the prevalence and type of BRAF mutation in our cases, however, there is no significant correlation between mutation B-RAF V600E gene and age, grade and clinical response of CRC patients at the center general hospital (RSUP) Prof. Dr. IGNG Ngoerah, Bali.