The assay of glandular kallikrein and prekallikrein in human mixed saliva

Jenzano, Joyce W.; Coffey, James C.; Heizer, William D.; Lundblad, Roger L.; Scicli, A. G.

doi:10.1016/0003-9969(88)90117-3

Cited by 18 publications

(14 citation statements)

References 19 publications

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“…A moderate elevation of BP (10 -20%) in this mouse experimental model has also been reported by other authors (2,17,23,25,32,44), but in a few studies, a greater increase in BP (ϳ50%) has been observed (11,20,22,24). It is now established that the elevation of BP is less marked in 2K1C mice than in 2K1C rats (24).…”

Section: Discussionsupporting

confidence: 86%

“…Four weeks after clipping, randomly selected mice (sham-TKϩ/ϩ, n ϭ 10; sham-TKϪ/Ϫ, n ϭ 9; 2K1C-TKϩ/ϩ, n ϭ 13; 2K1C-TKϪ/Ϫ, n ϭ 12) were housed individually in metabolic cages for 24-h urine collection for urinary sodium, potassium, chloride, and calcium assays (Konelab version 6.0.7). Urinary kallikrein activity was assessed by quantifying generation of p-nitroanilide with a spectrophotometer at 405 nm (model 3550; Bio-Rad Hercules, CA) after incubating the urine with the substrate S 2266 (H-D-Val-Leu-Arg-pNA; Chromogenix-Instrumentation Laboratory, Milan, Italy) (22).…”

Section: Methodsmentioning

confidence: 99%

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Tissue kallikrein deficiency and renovascular hypertension in the mouse

Griol-Charhbili¹,

Colucci²,

Vincent³

et al. 2009

American Journal of Physiology-Regulatory, Integrative and Comp

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The kallikrein kinin system (KKS) is involved in arterial and renal functions. It may have an antihypertensive effect in both essential and secondary forms of hypertension. The role of the KKS in the development of two-kidneys, one-clip (2K1C) hypertension, a high-renin model, was investigated in mice rendered deficient in tissue kallikrein (TK) and kinins by TK gene inactivation (TK-/-) and in their wild-type littermates (TK+/+). Four weeks after clipping the renal artery, blood flow was reduced in the clipped kidney (2K1C-TK+/+: -90%, 2K1C-TK-/-: -93% vs. sham-operated mice), and the kidney mass had also decreased (2K1C-TK+/+: -65%, 2K1C-TK-/-: -66%), whereas in the unclipped kidney, blood flow (2K1C-TK+/+: +19%, 2K1C-TK-/-: +17%) and kidney mass (2K1C-TK+/+: +32%, 2K1C-TK-/-: +30%) had both increased. The plasma renin concentration (2K1C-TK+/+: +78%, 2K1C-TK-/-: +65%) and renal renin content of the clipped kidney (2K1C-TK+/+: +58%, 2K1C-TK-/-: +65%) had increased significantly. There was no difference for these parameters between 2K1C-TK+/+ and 2K1C-TK-/- mice. Blood pressure monitored by telemetry and by plethysmography, rose immediately after clipping in both genotypes, and reached similar levels (2K1C-TK+/+: +24%, 2K1C-TK-/-: +21%). 2K1C-TK+/+ and 2K1C-TK-/- mice developed similar concentric left ventricular hypertrophy (+24% and +17%, respectively) with normal cardiac function. These findings suggest that in the context of chronic unilateral reduction in renal blood flow, TK and kinins do not influence the trophicity of kidneys, the synthesis and secretion of renin, blood pressure increase, and cardiac remodeling due to renin angiotensin system activation.

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Section: Discussionsupporting

confidence: 86%

Section: Methodsmentioning

confidence: 99%

Tissue kallikrein deficiency and renovascular hypertension in the mouse

Griol-Charhbili¹,

Colucci²,

Vincent³

et al. 2009

American Journal of Physiology-Regulatory, Integrative and Comp

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“…An early example of such testing comes from the work of Jenzano et al (1986a, b), Jenzano, Brown and Mauriello (1987), Jenzano et al (1988). They reported the use of saliva to detect kallikrein in patients with solid tumors that were remote from the oral cavity.…”

Section: Oncologymentioning

confidence: 99%

Saliva as a diagnostic fluid

2002

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In the last 10 years, the use of saliva as a diagnostic fluid has become somewhat of a translational research success story. Technologies are now available enabling saliva to be used to diagnose disease and predict disease progression. This review describes some important recent advances in salivary diagnostics and barriers to application and advancement. This review will also stimulate future research activity.

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“…Active tissue kallikrein is secreted into human parotid and submandibular saliva under reflex conditions (4), and kallikrein activity has been assayed in parotid saliva evoked by different stimuli (5, 6). Although active kallikrein is stored and secreted by the major human salivary glands, whole mouth saliva has been shown to contain inactive pre‐kallikrein (7). Tissue kallikrein levels in saliva can be determined by measuring its activity using an appropriate substrate and those of pre‐kallikrein estimated by examining the effect of trypsinisation.…”

mentioning

confidence: 99%

Levels of pre‐kallikrein in resting and stimulated human parotid and submandibular saliva

Francis¹,

Hector

Proctor³

2001

European J Oral Sciences

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Salivary tissue kallikrein is stored in an active form in human salivary glands. Pre-kallikrein has been demonstrated in mixed saliva, but it is not clear if the various salivary glands contribute equally. This study set out to determine if pre-kallikrein is present in human parotid and submandibular salivas at rest, whether levels change during stimulation, and to compare the pattern of pre-kallikrein and kallikrein secretion with that of total protein. Resting and citric acid-stimulated parotid and submandibular, and gum-stimulated parotid saliva samples were collected from 6 healthy subjects. Salivary flows were determined gravimetrically. Total protein concentration and kallikrein enzymic activity were assayed using standard techniques. Pre-kallikrein was assayed following trypsinisation of duplicate samples. Pre-kallikrein was present in parotid and submandibular ductal saliva. Proportions of pre-kallikrein and active kallikrein were similar in salivas secreted at rest and during stimulation, and both outputs mirrored protein output in both major glands. Gum-stimulated parotid saliva showed lower activity than resting, and no differences were seen between resting and stimulated submandibular samples.

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The assay of glandular kallikrein and prekallikrein in human mixed saliva

Cited by 18 publications

References 19 publications

Tissue kallikrein deficiency and renovascular hypertension in the mouse

Tissue kallikrein deficiency and renovascular hypertension in the mouse

Saliva as a diagnostic fluid

Levels of pre‐kallikrein in resting and stimulated human parotid and submandibular saliva

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