Levels of pre‐kallikrein in resting and stimulated human parotid and submandibular saliva

Francis, Carol; Hector, Mark; Proctor, Gordon

doi:10.1034/j.1600-0722.2001.00091.x

Cited by 4 publications

(4 citation statements)

References 11 publications

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“…Submandibular/sublingual gland saliva was collected as described previously [10] using a suction appliance made from soft polyethylmethacrylate (Eversoft; Myerson, Chicago, IL, U.S.A.) based on the design of Truelove et al [11]. The suction device was placed on the floor of the mouth over the papillae of the submandibular ducts.…”

Section: Collection Of Salivamentioning

confidence: 99%

A statherin and calcium enriched layer at the air interface of human parotid saliva

Proctor

Hamdan

Carpenter

et al. 2005

Biochemical Journal

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Parotid saliva placed in 35-mm-diameter tissue culture dishes developed increasing surface viscoelasticity at the interface with air. A surface layer became visible with time, and was collected and analysed by protein electrophoresis which indicated that a single protein (pI 4.2; molecular mass approx. 6 kDa) predominated. Western blot analysis demonstrated that the major protein band reacted with an antiserum directed against the C-terminal of the calcium-binding salivary protein statherin. Matrix-assisted laser-desorption ionization-time-of-flight MS analysis gave a molecular mass of 5380 Da for the protein, corresponding to the molecular mass of statherin. Staining of film protein in electrophoresis gels was compared with statherin synthesized on a solid phase, and the mean statherin content of film formed from 1 ml of parotid saliva was measured as 7 nmol. The mean calcium content of the surface layer was 250 nmol. Surface rheology was greatly decreased in the presence of EDTA, whereas surface tension of saliva was unaffected by calcium chelation, suggesting that protein accumulated at the surface was unaffected. The results suggest that a layer rich in statherin forms at the interface of saliva and air, and that the surface rheology developed is dependent upon protein interactions mediated by calcium. The surface layer may enhance the function of saliva as a protective layer on the mucosal surfaces and teeth.

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Section: Collection Of Salivamentioning

confidence: 99%

A statherin and calcium enriched layer at the air interface of human parotid saliva

Proctor

Hamdan

Carpenter

et al. 2005

Biochemical Journal

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“…Prekallikrein, the precursor of kallikrein, is present in parotid and submandibular ductal saliva. The proportions of prekallikrein and active kallikrein are similar in salivas secreted at rest and during stimulation (Francis, Hector, & Proctor, 2001).…”

Section: Introductionmentioning

confidence: 81%

Salivary prekallikrein output during the ranger training‐induced stress

et al. 2004

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Saliva can be considered a mirror of the body, reflecting its general conditions. The present study was conducted to identify the changes in specific neurotransmitter and prekallikrein levels in saliva and the correlations between changes in neuroendocrine factors in plasma and saliva after intensive physical training of rangers of Japan Ground Self Defence Force (JGSDF). The subjects were 14 young uniformed males (aged 20-26 years old) who underwent ranger training under the control of the 4th Division of the JGSDF. Before training began and after 30 days of hard training, the levels of prekallikrein, b-endorphin, serotonin, noradrenaline and dopamine in saliva and plasma were routinely measured. The level of prekallikrein in saliva was significantly increased after training, and the level of b-endorphin in saliva showed an upward trend after the training. The level of prekallikrein in plasma showed a significant decrease after the training, while the level of b-endorphin was unchanged. Average concentrations of values in serotonin, noradrenaline and dopamine in plasma were significantly increased after the training. These findings suggest that the comparison of levels of prekallikrein and b-endorphin in saliva and plasma may give useful insights for the estimation of stress-induced analgesia.

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“…After GCF collection, stimulated parotid saliva samples were obtained from each patient using Lashley cup (Lashley, 1916 ) during stimulation with 1% citric acid. Citric acid was applied to the dorsum of the tongue, held there by the subject for 10 s and 1 ml of saliva was collected from each patient as described in detail before (Francis et al, 2001 ). Saliva samples were then placed in sterile Eppendorf tubes and stored at −80°C until the laboratory analysis.…”

Section: Methodsmentioning

confidence: 99%

Using Salivary Nitrite and Nitrate Levels as a Biomarker for Drug-Induced Gingival Overgrowth

Sukuroglu

Güncü

Kılınç

et al. 2015

Front. Cell. Infect. Microbiol.

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Aim: Drug-induced gingival overgrowth has a multifactorial nature and the pathogenesis is still uncertain. It has been suggested that Nitric Oxide (NO) might play a role in the pathogenesis of drug-induced gingival overgrowth due to the contribution of NO to immune response and matrix degradation. NO levels in biological fluids have been used as a diagnostic biomarker in many diseases. The aim of this study is to determine whether NO levels in plasma, saliva, and gingival crevicular fluid (GCF) can serve as a potential biomarker for the evaluation of drug-induced gingival overgrowth risk.Materials and Methods: A total of 104 patients, receiving cyclosporine A (n = 35), phenytoin (n = 25), nifedipine (n = 26), or diltiazem (n = 18) participated in the study. The amount of gingival overgrowth was evaluated with two indices and was given as percentage. Periodontal clinical parameters including plaque index (PI), gingival index (GI), gingival bleeding time index (GBTI), and probing depth (PD) were also assessed. Saliva, GCF, and plasma samples were obtained from each participants. Nitrite and nitrate levels in saliva, GCF, and plasma were analyzed by Griess reagent.Results: Salivary nitrite and nitrate levels in responders were significantly higher than those in non-responders in only phenytoin group (p < 0.05). Nitrite and nitrate levels of gingival crevicular fluid and plasma did not significantly differ between responders and non-responders in all study groups (p > 0.05). Salivary nitrite levels exhibited a significant correlation with PD, GBTI, severity of gingival overgrowth (%GO), and GCF volume (p < 0.05). Additionally, a strong positive correlation was detected between saliva and plasma nitrate levels (p < 0.005). However, both nitrite and nitrate levels in GCF and plasma demonstrated no significant correlation with clinical parameters, GO severity, and GCF volume (p > 0.05).Conclusion: Salivary nitrite and nitrate levels could be used as periodontal disease biomarkers in phenytoin induced gingival overgrowth, and that saliva seems to have a better diagnostic potential than GCF and plasma for the evaluation of drug-induced gingival overgrowth risk. However, when all drug groups were considered, saliva nitrite and nitrate levels could not be used as a biomarker for drug-induced gingival overgrowth.

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Levels of pre‐kallikrein in resting and stimulated human parotid and submandibular saliva

Cited by 4 publications

References 11 publications

A statherin and calcium enriched layer at the air interface of human parotid saliva

A statherin and calcium enriched layer at the air interface of human parotid saliva

Salivary prekallikrein output during the ranger training‐induced stress

Using Salivary Nitrite and Nitrate Levels as a Biomarker for Drug-Induced Gingival Overgrowth

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