The asbestos-carbon nanotube analogy: An update

Kane, Agnes B.; Hurt, Robert H.; Gao, Huajian

doi:10.1016/j.taap.2018.06.027

Cited by 79 publications

(67 citation statements)

References 144 publications

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“…Similar biological outcomes were also observed after i.p. injection of long and rigid MWCNTs (Poland et al 2008 ), supporting the idea that these materials have asbestos-like pulmonary pathogenicity potential and should be regulated in the same way (Murphy et al 2011 ; Chernova et al 2017 ; Kane et al 2018 ). Using the same administration route, we have previously demonstrated that GO sheets of small lateral dimensions did not induce a granulomatous response (Ali-Boucetta et al 2013a ).…”

Section: Discussionmentioning

confidence: 80%

Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity

et al. 2018

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Graphene oxide (GO) is an oxidised form of graphene that has attracted commercial interest in multiple applications, including inks, printed electronics and spray coatings, which all raise health concerns due to potential creation of inhalable aerosols. Although a number of studies have discussed the toxicity of GO sheets, the in vivo impact of their lateral dimensions is still not clear. Here, we compared the effects of large GO sheets (l-GO, 1–20 µm) with those of small GO sheets (s-GO, < 1 µm) in terms of mesothelial damage and peritoneal inflammation, after intraperitoneal (i.p.) injection in mice. To benchmark the outcomes, long and rigid multi-walled carbon nanotubes (MWCNTs) that were shown to be associated with asbestos-like pathogenicity on the mesothelium were also tested. Our aim was to assess whether lateral dimensions can be a predictor of inflammogenicity for GO sheets in a similar fashion as length is for MWCNTs. While long MWCNTs dispersed in 0.5% BSA induced a granulomatous response on the diaphragmatic mesothelium and immune cell recruitment to the peritoneal cavity, GO sheets dispersed under similar conditions did not cause any response, regardless of their lateral dimensions. We further interrogated whether tuning the surface reactivity of GO by testing different dispersions (5% dextrose instead of 0.5% BSA) may change the biological outcome. Although the change of dispersion did not alter the impact of GO on the mesothelium (i.e. no granuloma), we observed that, when dispersed in protein-free 5% dextrose solution, s-GO elicited a greater recruitment of monocytic cells to the peritoneal cavity than l-GO, or when dispersed in protein-containing solution. Such recruitment coincided with the greater ability of s-GO to interact in vivo with peritoneal macrophages and was associated with a greater surface reactivity in comparison to l-GO. In conclusion, large dimension was not a determining factor of the immunological impact of GO sheets after i.p. administration. For an equal dose, GO sheets with lateral dimensions similar to the length of long MWCNTs were less pathogenic than the MWCNTs. On the other hand, surface reactivity and the ability of some smaller GO sheets to interact more readily with immune cells seem to be key parameters that can be tuned to improve the safety profile of GO. In particular, the choice of dispersion modality, which affected these two parameters, was found to be of crucial importance in the assessment of GO impact in this model. Overall, these findings are essential for a better understanding of the parameters governing GO toxicity and inflammation, and the rational design of safe GO-based formulations for various applications, including biomedicine.Electronic supplementary materialThe online version of this article (10.1007/s00204-018-2303-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 80%

Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity

et al. 2018

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“…In addition, MWCNTs were added into the culture, and inflammatory cytokines interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-α in the culture solution were measured by enzyme-linked immunosorbent assay (ELISA). Neither MWCNTs nor tattoo ink increased the inflammatory cytokines in the culture [16]. CNTs have demonstrated favorable osteogenic responses in bone-related cells, and we believe that CNTs can be applied to orthopedic and dental implants and bone regeneration therapy.…”

Section: In Vitro Biocompatibility Testing Of Cntsmentioning

confidence: 88%

“…CNTs and asbestos often differ in their surface charge, hydrophilicity/hydrophobicity, active metal properties, tensile strength, and bio-durability. Despite these differences, the fibrous morphology of the nanoparticles has led some researchers to believe that CNTs may potentially be carcinogenic like asbestos [16]. The pharmacokinetics of these nanoparticles, as they are implanted in the body as biomaterials, remains unknown, and it is unclear how CNTs affect the living body.…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility and Carcinogenicity of Carbon Nanotubes as Biomaterials

Aoki

2020

Nanomaterials

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With the development of nanotechnology in recent years, there have been concerns about the health effects of nanoparticles. Carbon nanotubes (CNTs) are fibrous nanoparticles with a micro-sized length and nano-sized diameter, which exhibit excellent physical properties and are widely studied for their potential application in medicine. However, asbestos has been historically shown to cause pleural malignant mesothelioma and lung cancer by inhalation exposure. Because carbon nanotubes are also fibrous nanotubes, some have raised concerns about its possible carcinogenicity. We have reported that there is no clear evidence of carcinogenicity by local and intravenous administration of multi-walled CNTs to cancer mice models. We firmly believe that CNTs can be a safe, new, and high-performance biomaterials by controlling its type, site of administration, and dosage.

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“…The unique physicochemical properties of multi-walled carbon nanotubes (MWNTs) lend themselves to a variety of industrial and biomedical applications (for recent reviews, see [ 1 , 2 , 3 , 4 , 5 , 6 , 7 , 8 , 9 , 10 , 11 , 12 , 13 , 14 , 15 ]). However, there are environmental health and safety (EH&S) concerns associated with MWNTs because of consistent reports that they can be toxic (for recent reviews, see [ 16 , 17 , 18 , 19 , 20 , 21 , 22 ]). Moreover, anthropogenic MWNTs have been found in the lungs of asthmatic Parisian children not known to have been exposed to a source of MWNTs, suggesting that MWNTs may be a previously unrecognized air pollutant [ 23 ].…”

Section: Introductionmentioning

confidence: 99%

The Importance of Evaluating the Lot-to-Lot Batch Consistency of Commercial Multi-Walled Carbon Nanotube Products

et al. 2020

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The biological response of multi-walled carbon nanotubes (MWNTs) is related to their physicochemical properties and a thorough MWNT characterization should accompany an assessment of their biological activity, including their potential toxicity. Beyond characterizing the physicochemical properties of MWNTs from different sources or manufacturers, it is also important to characterize different production lots of the same MWNT product from the same vendor (i.e., lot-to-lot batch consistency). Herein, we present a comprehensive physicochemical characterization of two lots of commercial pristine MWNTs (pMWNTs) and carboxylated MWNTs (cMWNTs) used to study the response of mammalian macrophages to MWNTs. There were many similarities between the physicochemical properties of the two lots of cMWNTs and neither significantly diminished the 24-h proliferation of RAW 264.7 macrophages up to the highest concentration tested (200 μg cMWNTs/mL). Conversely, several physicochemical properties of the two lots of pMWNTs were different; notably, the newer lot of pMWNTs displayed less oxidative stability, a higher defect density, and a smaller amount of surface oxygen species relative to the original lot. Furthermore, a 72-h half maximal inhibitory concentration (IC-50) of ~90 µg pMWNTs/mL was determined for RAW 264.7 cells with the new lot of pMWNTs. These results demonstrate that subtle physicochemical differences can lead to significantly dissimilar cellular responses, and that production-lot consistency must be considered when assessing the toxicity of MWNTs.

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The asbestos-carbon nanotube analogy: An update

Cited by 79 publications

References 144 publications

Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity

Immunological impact of graphene oxide sheets in the abdominal cavity is governed by surface reactivity

Biocompatibility and Carcinogenicity of Carbon Nanotubes as Biomaterials

The Importance of Evaluating the Lot-to-Lot Batch Consistency of Commercial Multi-Walled Carbon Nanotube Products

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