The aromatic cytokinin meta-topolin promotes in vitro propagation, shoot quality and micrografting in Corylus colurna L.

Gentile, A.; Frattarelli, A.; Nota, P.; Condello, E.; Caboni, E.

doi:10.1007/s11240-016-1150-y

Cited by 64 publications

(23 citation statements)

References 53 publications

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“…The chlorophyll (Chl) content is usually correlated to photosynthetic apparatus efficiency of in vitro plants (Martins et al, 2016;Martins et al, 2020). A pronounced decrease in Chl content may correspond to pronounced impairment of photosystem II (PSII) function (Janečková et al, 2019). In fact, lower Chl content induced by previous BAP exposure can reduce the photosynthetic performance during in vitro culture of plants (Martins et al, 2018b).…”

Section: Discussionmentioning

confidence: 99%

“…High endogenous CK content can cause hyperaccumulation of phenolic compounds and increase the activity of some enzymes of the antioxidant system (Mamedes-Rodrigues et al, 2019). Plants with high antioxidant system activity may present low photosynthetic pigment content due to the action of reactive oxygen species (ROS) (Gentile et al, 2017;Rodrigues et al, 2017). Likewise, high concentrations of exogenous AUX supplemented in the culture medium can increase the antioxidant system's activity (peroxidase) (Larraburu et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

“…Anatomical studies can be used to assess the effect of in vitro conditions on the success of the transfer to ex vitro conditions (Martins et al, 2016;Martins et al, 2018aMartins et al, , 2018bFaria et al, 2018). Analyses of photosynthetic pigment content are also useful to verify the physiological status of plants (Martins et al, 2018b;Janečková et al, 2019). In this respect, chlorophyll content is a good first indicator of photosynthetic potential, and it can be directly related to photosynthetic apparatus performance of in vitro bromeliads (Martins et al, 2018b).…”

Section: Introductionmentioning

confidence: 99%

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Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP

et al. 2020

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During in vitro propagation, cytokinins (CKs) and auxins (AUXs), such as 6-benzylaminopurine (BAP) and 1-naphthaleneacetic acid (NAA), are often used to induce adventitious shoots and roots, respectively. However, it is not clear how CKs affect plants over a long period of in vitro propagation as well as the synergy of direct exposure to AUX with previous CK treatments. The aim was to assess the physiological and anatomical responses of Alcantarea imperialis in function of the interaction of both previous BAP treatments and direct NAA exposure during in vitro propagation. Plants previously grown in vitro were transferred to media containing 0, 5, 10 or 15 μM BAP. After 60 days, the adventitious shoots from each previous BAP treatment were subcultured in media with 0, 2 or 4 μM NAA. Pigment content, anatomical and growth traits were assessed in the plants from each treatment. Both previous BAP treatments and direct NAA exposure altered the anatomy and pigment contents of plants as well as their growth traits. BAP induced negative effects over the long term on physiological status as well as changed the plants’ anatomy. NAA supplementation in the medium can partially reverse the negative effects induced by BAP. The application of 2 μM NAA during in vitro rooting improved the plants’ quality.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP

et al. 2020

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“…However, in our study, mT had highly significant effects (P < 0.05) in addition with CPPU on plant regeneration from shoot-tip explants. Similarly, the aromatic cytokinin mT promotes in vitro propagation, shoot quality and micrografting was produced in Corylus colurna [28]. These finding are in contrast with Basalma et al [14] reports that have proved that the highest percentage of shoot regeneration per explants of safflower cv.…”

Section: Shoot Induction and Multiplication Via Micropropagationmentioning

confidence: 80%

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (<i>Carthamus tinctorius</i> L.) cv. NARI-H-15

Vijayakumar¹,

Ponmanickam²,

Samuel³

et al. 2017

AJPS

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The effect of meta-Topolin (mT) was assessed to develop a reliable protocol for efficient plant regeneration of safflower (Carthamus tinctorius L.) cv.NARI-H-15. For micropropagation, 7 -9 days old shoot-tip explants cultured on MS basal medium supplemented with 3.0 mg/L meta-Topolin (mT) + 0.5 mg/L CPPU showed 97.7% adventitious shoot formation (42.4 shootlets) than node after 45 days of culture. For organogenesis, the seedling explants of immature leaf cultured on 1.5 mg/L CPPU or 1.5 mg/L NAA fortified medium produced high amount of callus than cotyledon and stem calli after 60 days of culture. However, MS basal medium fortified with 4.0 mg/L mT + 1.5 mg/L CPPU was found beneficial to stimulate 100% organogenic response (74.7 shootlets) from immature leaf calli than cotyledon and stem derived calli after 45 days of culture. The healthy plantlets obtained from micropropagation and organogenesis process cultured on 1/4 MS basal salts, 1.5% sucrose (w/v) and 0.8% agar (w/v) medium supplemented with NAA (1.5 mg/L) and mT (0.1 mg/L) produced maximum of 96% (12.8 rootlets) and 84% (7.3 rootlets) adventitious rooting, respectively than mT and CPPU tested medium. However, maximum of 67% and 42% survival rate was noticed when in vitro raised plants from micropropagation and organogenesis were hardened in pots containing soil mix and maintained under green house condition. This optimized regeneration protocol might be helpful in regeneration of new genotypes and cultivars of safflower to improve agronomic traits through in vitro selection process and Agrobacterium-mediated genetic transformation system. Keywords

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“…(Bairu et al, 2007), Musa spp. (Bairu et al, 2008), Pyrus (Dimitrova et al, 2016), Corylus (Gentile et al, 2017) while it was observed to reduce shoot formation in Citrus (Niedz and Evens, 2010).…”

Section: Methodsmentioning

confidence: 99%

Micropropagation of Camptotheca Acuminata Decne (Nyssaceae) - Endangered Ornamental and Medicinal Tree

Nacheva¹,

Dimitrova²,

Ivanova³

et al. 2020

Acta Univ. Agric. Silvic. Mendelianae Brun.

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Camptotheca acuminata Decne (Nyssaceae) (happy tree, tree of life, cancer tree) is a rapidly growing deciduous and endangered tree endemic to east Tibet and southern China (Liu et al., 2002). C. acuminata is an ornamental tree with monopodial growth, beautiful leaves and shade- and cold- tolerant in their natural environment. Recently, its demand has grown rapidly due to secondary metabolites production like Camptothecin and its derivatives with anti cancer and antivirus activity. The aim of the present study was to develop an efficient protocol for in vitro micropropagation of this valuable plant. Different cultural media based on both MS (Murashige and Skoog, 1962) or DKW (Driver and Kuniyuki, 1984) formulations with different cytokinins (6-benzylaminopurine, BAP or 2-isopentenyladenine, 2iP or meta-topolin, mT) have been involved. The aromatic cytokinin meta-topolin stimulated plant growth. The optimum multiplication rate of Camptotheca shoots occurred on the DKW basal medium, supplemented with 2.5 µM meta-topolin, grown under mixed LED light. The highest number of roots per plantlets was recorded on the rooting medium with 0.3 mgl-1 NAA.

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The aromatic cytokinin meta-topolin promotes in vitro propagation, shoot quality and micrografting in Corylus colurna L.

Cited by 64 publications

References 53 publications

Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP

Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (<i>Carthamus tinctorius</i> L.) cv. NARI-H-15

Micropropagation of Camptotheca Acuminata Decne (Nyssaceae) - Endangered Ornamental and Medicinal Tree

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The aromatic cytokinin meta-topolin promotes in vitro propagation, shoot quality and micrografting in Corylus colurna L.

Cited by 64 publications

References 53 publications

Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP

Modulation of the anatomical and physiological responses of in vitro grown Alcantarea imperialis induced by NAA and residual effects of BAP

Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (&lt;i&gt;Carthamus tinctorius&lt;/i&gt; L.) cv. NARI-H-15

Micropropagation of Camptotheca Acuminata Decne (Nyssaceae) - Endangered Ornamental and Medicinal Tree

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Influence of Meta-Topolin on Efficient Plant Regeneration via Micropropagation and Organogenesis of Safflower (<i>Carthamus tinctorius</i> L.) cv. NARI-H-15