Camptotheca acuminata Decne (Nyssaceae) (happy tree, tree of life, cancer tree) is a rapidly growing deciduous and endangered tree endemic to east Tibet and southern China (Liu et al., 2002). C. acuminata is an ornamental tree with monopodial growth, beautiful leaves and shade- and cold- tolerant in their natural environment. Recently, its demand has grown rapidly due to secondary metabolites production like Camptothecin and its derivatives with anti cancer and antivirus activity. The aim of the present study was to develop an efficient protocol for in vitro micropropagation of this valuable plant. Different cultural media based on both MS (Murashige and Skoog, 1962) or DKW (Driver and Kuniyuki, 1984) formulations with different cytokinins (6-benzylaminopurine, BAP or 2-isopentenyladenine, 2iP or meta-topolin, mT) have been involved. The aromatic cytokinin meta-topolin stimulated plant growth. The optimum multiplication rate of Camptotheca shoots occurred on the DKW basal medium, supplemented with 2.5 µM meta-topolin, grown under mixed LED light. The highest number of roots per plantlets was recorded on the rooting medium with 0.3 mgl-1 NAA.
This study was conducted to investigate the effects of different treatment with ultrasound and stratification of Laurocerasus officinalis L. seeds, conducted in greenhouse conditions during the period 2013-2015. The Laurocerasus officinalis L. is a species with an economic importance for the pharmaceutical industry, and it is also often used in ornamental horticulture. The determination of appropriate conditions for propagation by seeds has an effect on the cost of production. The seeds were treated with 0, 5, 10 and 5 min ultrasound and 2 months stratification. The results showed that the percentage of germinated seeds and quality root system are dependent on the exposition time. The largest number of germinated seeds was obtained with ultrasound treatment of 10 min. The stratification resulted in the obtaining of roots with the greatest length. High doses of ultrasound will provide the breaking of seeds and weak, brittle root system, which reduces the chances of successful adaptation in replanting.
An experiment was undertaken studying the effects of genotype, treatment with growth regulators and duration of rooting period on the percentage of rooting and quality of the root system of green cuttings of giant sequoia (Sequoiadendron giganteum (Lindl.) J.Buchh.). Cuttings were taken from 18 ten-year seedlings of 10 species of origin. The duration of rooting period (3 months vs. 5 months) was statistically reliable for root- and callus formation rates. The extension of rooting period for two month affords 57.76% more rooted cuttings. The genotype is the main factor influencing the percentage of rooting, number of roots formed and the length of the main root. Treatment with auxin also increase the percentage of rooting, number of roots formed and the length of the main root. Treatment with 4000 ppm indole butyric acid (IBA) gives good results at the end of the third and fourth month, but a five-month rooting period cuttings treated with 4000 ppm IBA showed the highest percentage of rooting.
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