The arG gene of Streptomyces clavuligerus has low homology to unstable arG from other actinomycetex: effect of amplificatio on clavulcanic acid biosynthesis

Rodrı́guez-Garcı́a, Antonio; Martı́n, Juan F.; Liras, Paloma

doi:10.1016/0378-1119(95)00667-2

Cited by 10 publications

(5 citation statements)

References 21 publications

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“…As shown in this article, in Streptomyces clavuligerus, argR is linked to argG. It forms part of a cluster of arginine biosynthesis genes containing (at least) argRGH (Rodríguez-García et al, 1995). In S. coelicolor, the unstable argG is not preceded by argR (Ishihara et al, 1985).…”

Section: Discussionmentioning

confidence: 86%

“…A short incomplete open reading frame, ORF1 in Fig. 2, was found upstream of the argG gene in a 6.1 kb Bgl II-Sau 3A DNA fragment from S. clavuligerus present in plasmid pULAR10 (Rodríguez-García et al, 1995). To obtain the complete sequence of ORF1, additional clones were isolated from plasmid pULAR10 and an 821-nt DNA fragment upstream of argG was sequenced.…”

Section: Resultsmentioning

confidence: 99%

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Arginine boxes and the argR gene in Streptomyces clavuligerus: evidence for a clear regulation of the arginine pathway

Rodrı́guez-Garcı́a

Ludovice

Martı́n

et al. 1997

Molecular Microbiology

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SummaryThe argR gene of Streptomyces clavuligerus has been located in the upstream region of argG. It encodes a protein of 160 amino acids with a deduced M r of 17 117 for the monomer. Transformants containing the amplified argR gene showed lower activity (50%) of the biosynthetic ornithine carbamoyltransferase (OTC) activity and higher levels (380%) of the catabolic ornithine aminotransferase (OAT) activity than control strains. Amplification of an arginine (ARG) box-containing sequence results in a 2-to 2.5-fold derepression of ornithine acetyltransferase and OTC, suggesting that the repressor is titrated out. Footprinting experiments using the pure homologous arginine repressor (AhrC) of B. subtilis showed a protected 38 nt region (ARG box) in the coding strand upstream of argC. The protected region contained two tandemly repeated imperfect palindromic 18-nt ARG boxes. The repressor-operator interaction was confirmed by bandshift experiments of the DNA fragment containing the protected region. By computer analysis of the Streptomyces sequences available in the databases, a consensus ARG box has been deduced for the genus Streptomyces. This is the first example of a clear regulation of an amino acid biosynthetic pathway in Streptomyces species, challenging the belief that actinomycetes do not have a well-developed regulatory system of these pathways.

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Section: Discussionmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

Arginine boxes and the argR gene in Streptomyces clavuligerus: evidence for a clear regulation of the arginine pathway

Rodrı́guez-Garcı́a

Ludovice

Martı́n

et al. 1997

Molecular Microbiology

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“… b . The attB gene product is also homologous to ArgG of Streptomyces clavuligerus , which is involved in clavulanic acid biosynthesis (54.2% aa similarity, E ‐value 4.0e −7 7 ; Rodríguez‐García et al ., 1995). …”

Section: Resultsmentioning

confidence: 99%

The att locus of Rhodococcus fascians strain D188 is essential for full virulence on tobacco through the production of an autoregulatory compound

Maes

Vereecke

Ritsema

et al. 2001

Molecular Microbiology

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SummaryThe ability of Rhodococcus fascians strain D188 to provoke leafy gall formation on a variety of plant species is correlated with the linear plasmid pFiD188, on which different pathogenicity loci were identified. The att locus affects the severity of symptom development on tobacco, whereas the fas locus is essential for virulence. To gain insight into the function of the att locus, sequence and expression analyses were performed. The att locus contains nine open reading frames homologous to arginine and b-lactam biosynthetic genes. att gene expression is transcriptionally induced by leafy gall extracts, but not by extracts of uninfected plants, and depends on the attR gene that encodes a LysR-type transcriptional regulator. The att locus proves to be essential for the formation of inducing factors (IFs) that are present in gall extracts. Because the induction of the fas locus also requires the presence of IFs in gall extracts, the att locus is proposed to play an important role in regulating the expression of the virulence loci of R. fascians.

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“…ArgR, in conjunction with its corepressor arginine, binds to conserved 18-bp imperfect palindromic DNA sequences termed ARG boxes that are located upstream from the E. coli arg regulons. Recently, the existence of arginine repression similar to ArgR/AhrC has been extended to another grampositive bacterium (35,43). In this context, the aim of this work was to elucidate arginine biosynthesis in an arginine prototrophic L. plantarum strain, strain CCM 1904, a step in our study of the interactions between arginine and pyrimidine metabolic pathways.…”

mentioning

confidence: 99%

Arginine biosynthesis and regulation in Lactobacillus plantarum: the carA gene and the argCJBDF cluster are divergently transcribed

et al. 1997

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The arG gene of Streptomyces clavuligerus has low homology to unstable arG from other actinomycetex: effect of amplificatio on clavulcanic acid biosynthesis

Cited by 10 publications

References 21 publications

Arginine boxes and the argR gene in Streptomyces clavuligerus: evidence for a clear regulation of the arginine pathway

Arginine boxes and the argR gene in Streptomyces clavuligerus: evidence for a clear regulation of the arginine pathway

The att locus of Rhodococcus fascians strain D188 is essential for full virulence on tobacco through the production of an autoregulatory compound

Arginine biosynthesis and regulation in Lactobacillus plantarum: the carA gene and the argCJBDF cluster are divergently transcribed

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